##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166290
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80123667652992	34.0	31.0	34.0	31.0	34.0
2	33.01750273509087	34.0	33.0	34.0	31.0	34.0
3	33.14635713593286	34.0	33.0	34.0	31.0	34.0
4	36.43931375762248	37.0	37.0	37.0	35.0	37.0
5	36.408672892364365	37.0	37.0	37.0	35.0	37.0
6	36.44884849212248	37.0	37.0	37.0	35.0	37.0
7	36.37224286683685	37.0	37.0	37.0	35.0	37.0
8	36.385325602758634	37.0	37.0	37.0	35.0	37.0
9	38.16255256683085	39.0	39.0	39.0	37.0	39.0
10	38.14768798268007	39.0	39.0	39.0	37.0	39.0
11	38.180911604632804	39.0	39.0	39.0	37.0	39.0
12	38.08515295736028	39.0	39.0	39.0	37.0	39.0
13	38.11400597334614	39.0	39.0	39.0	37.0	39.0
14	39.564558761753965	41.0	39.0	41.0	37.0	41.0
15	39.543234746963705	41.0	39.0	41.0	37.0	41.0
16	39.47414473593101	41.0	39.0	41.0	37.0	41.0
17	39.38763369631951	41.0	39.0	41.0	36.0	41.0
18	39.2764149767575	41.0	39.0	41.0	36.0	41.0
19	39.33303020371234	41.0	39.0	41.0	36.0	41.0
20	39.557854673197035	41.0	39.0	41.0	37.0	41.0
21	39.56143360307254	41.0	39.0	41.0	37.0	41.0
22	39.5858666198893	41.0	40.0	41.0	37.0	41.0
23	39.52776405744383	41.0	40.0	41.0	37.0	41.0
24	39.48419048234539	41.0	40.0	41.0	37.0	41.0
25	39.46171980667408	41.0	39.0	41.0	36.0	41.0
26	39.31575827797756	41.0	39.0	41.0	36.0	41.0
27	39.22232480415826	41.0	39.0	41.0	36.0	41.0
28	39.193689672204556	41.0	39.0	41.0	36.0	41.0
29	39.08647457173324	41.0	39.0	41.0	35.0	41.0
30	39.00148041120995	40.0	39.0	41.0	35.0	41.0
31	38.83886090966583	40.0	39.0	41.0	35.0	41.0
32	38.73255981424463	40.0	38.0	41.0	35.0	41.0
33	38.671850029312786	40.0	38.0	41.0	35.0	41.0
34	38.560536677914776	40.0	38.0	41.0	35.0	41.0
35	38.460809956192385	40.0	38.0	41.0	35.0	41.0
36	38.421663766162425	40.0	38.0	41.0	35.0	41.0
37	38.34903221636992	40.0	38.0	41.0	35.0	41.0
38	38.277992789515714	40.0	38.0	41.0	34.0	41.0
39	38.17177940164982	40.0	38.0	41.0	34.0	41.0
40	38.11484888911457	40.0	38.0	41.0	34.0	41.0
41	37.99639244976434	40.0	38.0	41.0	34.0	41.0
42	37.898023810293175	40.0	38.0	41.0	34.0	41.0
43	37.608900470389464	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	6.0
16	7.0
17	25.0
18	62.0
19	105.0
20	200.0
21	401.0
22	736.0
23	1295.0
24	2115.0
25	3379.0
26	4790.0
27	6873.0
28	9739.0
29	13110.0
30	17402.0
31	22123.0
32	28506.0
33	37009.0
34	50370.0
35	71880.0
36	113285.0
37	207164.0
38	391284.0
39	1184418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.802727243351534	19.214278790005032	11.888574475254929	24.094419491388503
2	18.822318341496292	21.884466068716563	34.95016826002059	24.34304732976656
3	19.6280738035997	22.624671673690965	30.445231247893865	27.30202327481547
4	13.040082352778251	16.171796019923462	36.32186826325192	34.466253364046366
5	14.367328474027023	36.34550314131534	34.715850601719985	14.571317782937648
6	33.76293109417484	36.026570773072855	15.611252417728005	14.599245715024303
7	29.037801956340104	30.58620960259245	21.49850666346611	18.87748177760134
8	26.313420640819096	33.7686090043346	20.678717992512542	19.239252362333758
9	24.95903133929437	13.914388193639818	20.7409903567851	40.38559011028071
10	15.998181222274027	27.168707790739006	35.16809845403893	21.665012532948037
11	34.17935733442891	22.70245442669264	21.645116766453246	21.473071472425207
12	20.217468575306167	25.579169917231763	31.151000096939928	23.05236141052214
13	29.833540292389294	20.807740422565768	25.601004482317695	23.757714802727243
14	21.66953639632736	22.05124890942579	26.65483384034455	29.624380853902295
15	23.15594864953446	28.86312543565266	25.044707772274254	22.936218142538625
16	24.176541460284636	26.865470458710515	25.162882162591345	23.79510591841351
17	22.53701028024872	26.82941803728956	26.756066823924773	23.877504858536945
18	21.93990647604891	25.599665788052384	28.37616385617807	24.084263879720627
19	24.80129622534379	25.952988750351984	27.937579917739548	21.30813510656468
20	24.17451033795106	26.236145668400813	27.772505066265367	21.81683892738276
21	22.368103993463478	26.48015731965711	27.603598779480127	23.54813990739929
22	23.906540675532824	25.228339695977915	27.792216185275286	23.072903443213974
23	21.435449547382852	25.936370476713645	28.48104362758449	24.147136348319016
24	22.30624708603188	26.98673769439918	27.66402466890398	23.042990550664964
25	22.958514326336733	26.4887895895748	27.681843151193974	22.870852932894486
26	23.832774005327078	26.4153922143388	26.59934727114098	23.15248650919314
27	23.187615693189738	26.213987970216362	26.608348836028416	23.990047500565485
28	22.735044707772275	26.144883649003596	27.842347977417614	23.27772366580652
29	23.0001061723038	26.951146891690403	28.227199497758843	21.82154743824696
30	23.20423396682808	26.002705085653353	26.957101773077476	23.835959174441097
31	22.26271644147367	25.422311878834318	28.496969473154564	23.818002206537443
32	21.749811890374787	26.66725138370209	28.05464642314741	23.528290302775716
33	22.38896915925384	25.561120625585676	28.189208277746747	23.860701937413737
34	23.428488337203238	25.895009440102662	28.392643644202764	22.28385857849134
35	23.154102174685754	25.9171209764159	27.147196358751597	23.781580490146748
36	22.13923343596656	25.561074463714462	27.05731919549091	25.24237290482807
37	21.574719912846387	24.906314482363857	29.906476048913117	23.612489555876635
38	21.62434392440532	24.214255709069423	29.11452298630377	25.04687738022148
39	21.23736895798808	25.4319597099188	30.35950865304276	22.97116267905036
40	22.189734523078627	24.52275549441672	29.72616777993713	23.561342202567523
41	21.00794445803655	24.964386116355612	29.98596679114985	24.04170263445799
42	20.114204469392373	25.987240858795452	29.834371206071207	24.064183465740967
43	21.043719908230198	23.934376283877043	29.60434660179385	25.417557206098905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3811.0
1	3013.5
2	2216.0
3	3661.5
4	5107.0
5	5107.0
6	6174.0
7	7241.0
8	6316.5
9	5392.0
10	6478.0
11	7564.0
12	7564.0
13	11956.5
14	16349.0
15	20216.5
16	24084.0
17	23816.0
18	23548.0
19	23548.0
20	28464.0
21	33380.0
22	32295.0
23	31210.0
24	35773.0
25	40336.0
26	40336.0
27	43535.0
28	46734.0
29	51638.5
30	56543.0
31	63184.5
32	69826.0
33	69826.0
34	87207.0
35	104588.0
36	108795.0
37	113002.0
38	110040.0
39	107078.0
40	107078.0
41	106879.0
42	106680.0
43	116534.5
44	126389.0
45	121475.0
46	116561.0
47	116561.0
48	115994.0
49	115427.0
50	123340.5
51	131254.0
52	142420.5
53	153587.0
54	153587.0
55	158094.0
56	162601.0
57	143263.0
58	123925.0
59	120909.0
60	117893.0
61	117893.0
62	107254.0
63	96615.0
64	76687.0
65	56759.0
66	51169.0
67	45579.0
68	45579.0
69	40644.0
70	35709.0
71	37518.5
72	39328.0
73	34415.0
74	29502.0
75	29502.0
76	17350.5
77	5199.0
78	3837.5
79	2476.0
80	1917.0
81	1358.0
82	1358.0
83	1024.0
84	690.0
85	537.5
86	385.0
87	280.0
88	175.0
89	175.0
90	138.5
91	102.0
92	73.5
93	45.0
94	36.0
95	27.0
96	27.0
97	18.0
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2166290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.10321146130313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31576237605965	42.065998113021564
2	11.318768096774532	11.568507990618409
3	2.9688264444847117	4.551496967532328
4	1.112983369492757	2.2750809793640814
5	0.5766230953541128	1.4733645987676192
6	0.31074213661642974	0.9527952670467932
7	0.2068922044471943	0.7400999251492091
8	0.14786103656776606	0.6044939054888019
9	0.11492861198982882	0.5285899045323251
>10	0.7566696990670063	7.643962948598334
>50	0.08644162679289101	3.065207300826351
>100	0.059489899530701676	6.221833312151915
>500	0.011732844561049196	4.39292054895455
>1k	0.01164189227763021	11.389810719385824
>5k	4.547614170949301E-4	1.513699105073406
>10k+	1.8190456683797203E-4	1.0121384134884543
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11262	0.5198749936527427	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10514	0.48534591398196913	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7963	0.3675869805058418	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	7277	0.33591993685056015	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6037	0.2786792165407217	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	5744	0.26515378827396147	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	5546	0.2560137377728744	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	4626	0.21354481625267163	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	4378	0.20209667219070393	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	4269	0.19706502822798422	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	3998	0.18455516112801149	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	3996	0.18446283738557626	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	3533	0.16308989101182206	No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA	3487	0.16096644493581191	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	3473	0.16032017873876536	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	3317	0.15311892682881792	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	3298	0.15224185127568332	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3150	0.14540989433547677	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	3140	0.14494827562330068	No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC	3130	0.14448665691112456	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	3044	0.14051673598640993	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2976	0.13737772874361237	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2940	0.13571590137977835	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2939	0.13566973950856073	No Hit
CCCCATCTCTCTTCCCTAATCCTCCAAGTCTTCAGCGAGACCC	2884	0.13313083659159208	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	2759	0.12736060268939062	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2757	0.1272682789469554	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2684	0.12389846234806974	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	2674	0.12343684363589362	No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC	2655	0.122559768082759	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2617	0.12080561697648975	No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA	2587	0.11942076083996142	No Hit
CTTTCACATCCTGTTAGCTTTCTTAACTAGTATTGTCCATATC	2580	0.11909762774143812	No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA	2522	0.11642023921081665	No Hit
GGCTTGAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGC	2474	0.11420446939237128	No Hit
CCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTCCCTAATC	2424	0.11189637583149072	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2410	0.11125010963444415	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	2409	0.11120394776322653	No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT	2398	0.1106961671798328	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	2385	0.11009606285400386	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2354	0.10866504484625789	No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC	2346	0.10829574987651698	No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC	2315	0.10686473186877103	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	2314	0.10681856999755343	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2300	0.10617230380050686	No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG	2282	0.10534139011858985	No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC	2266	0.10460280017910806	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2238	0.10331026778501494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.232374243522336E-5	0.0
5	0.0	0.0	0.0	9.232374243522336E-5	0.0
6	0.0	0.0	0.0	9.232374243522336E-5	0.0
7	0.0	0.0	0.0	9.232374243522336E-5	0.0
8	0.0	0.0	0.0	9.232374243522336E-5	0.0
9	0.0	0.0	0.0	1.3848561365283502E-4	0.0
10	0.0	0.0	0.0	2.3080935608805838E-4	0.0
11	0.0	0.0	0.0	3.692949697408934E-4	0.0
12	0.0	0.0	0.0	3.692949697408934E-4	0.0
13	0.0	0.0	0.0	4.6161871217611676E-4	0.0
14	0.0	0.0	0.0	5.077805833937285E-4	0.0
15	0.0	0.0	0.0	5.539424546113401E-4	0.0
16	0.0	0.0	0.0	6.924280682641752E-4	0.0
17	0.0	0.0	0.0	7.847518106993985E-4	0.0
18	4.616187121761168E-5	0.0	0.0	8.770755531346218E-4	0.0
19	4.616187121761168E-5	0.0	0.0	0.001015561166787457	0.0
20	4.616187121761168E-5	0.0	0.0	0.0013848561365283503	0.0
21	4.616187121761168E-5	0.0	0.0	0.0019387985911396904	0.0
22	4.616187121761168E-5	0.0	0.0	0.0030466835003623706	0.0
23	4.616187121761168E-5	0.0	0.0	0.004662348992978779	0.0
24	4.616187121761168E-5	0.0	0.0	0.008632269917693383	0.0
25	4.616187121761168E-5	0.0	0.0	0.01486412253207096	0.0
26	4.616187121761168E-5	0.0	0.0	0.029081978867095355	0.0
27	4.616187121761168E-5	0.0	0.0	0.08886160209390248	0.0
28	4.616187121761168E-5	0.0	0.0	0.21719160407886295	0.0
29	4.616187121761168E-5	0.0	0.0	0.3539230666254287	0.0
30	4.616187121761168E-5	0.0	0.0	0.4910238241417354	0.0
31	4.616187121761168E-5	0.0	0.0	0.8088021456037742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2695	0.0	29.174398	1
CGTGCGA	70	5.105365E-6	21.142857	10
GTATCAA	4340	0.0	18.07373	2
CGGTTAC	65	0.0015805443	17.076923	22
TTAACGG	130	2.5918598E-7	15.653846	35
CCGTGTT	140	6.00141E-7	14.535714	9
TAACGGC	140	6.00141E-7	14.535714	36
CGCTAAG	90	8.2781824E-4	14.388888	35
ATTACGT	105	1.6566986E-4	14.095238	17
GTACTAT	265	0.0	13.962264	6
ACGCCGG	80	0.006300994	13.875	14
CTATCGC	80	0.006300994	13.875	30
ACATTCG	80	0.006300994	13.875	28
ACGACCG	80	0.006300994	13.875	30
TACGTTA	95	0.0012456961	13.631579	19
CGACCAA	150	1.3069093E-6	13.566667	12
CTTAACG	110	2.4579334E-4	13.454545	30
ACGACCA	180	5.1650204E-8	13.361111	11
CTATACC	225	4.0745363E-10	13.155556	4
CGTCTAA	85	0.009408243	13.058824	1
>>END_MODULE