FastQCFastQC Report
Fri 10 Feb 2017
ERR1630038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293983
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50460.3899587552541262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.3730342670653324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35770.27643330708363245No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.18717402006054173No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC18270.14119196310925258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.025.6590041
TACGCAG500.007035347618.510
GTATAGG1351.1532393E-917.8148161
TTAGACT1258.580173E-917.764
ACGTTAC752.0675201E-417.26666626
GACGTTA752.0675201E-417.26666625
TACCCCG1201.0414624E-716.9583345
TAGACTA1201.0414624E-716.9583345
GTATCAA20100.016.6592032
GACAGGC6000.016.3416657
CGTGCGA2050.016.24390410
ATTCGCG803.3829754E-416.187530
TATCCGG700.002592729915.85714210
CGAGAAT2250.015.62222214
GTATTGG1853.0559022E-1015.01
CGTTACT1001.0935323E-414.827
CGACGTT750.004104777714.79999924
ACGCTAA750.004104777714.79999934
AACTACG750.004104777714.7999997
GCATCAG5550.014.66666715
Produced by FastQC (version 0.11.5)