FastQCFastQC Report
Fri 10 Feb 2017
ERR1630037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112168
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108680.3492099398233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103760.33340102462334936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78410.2519465530138476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55340.17781816405798145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.026.6552331
CGCGAAT1205.18412E-918.528
GTATCAA44700.017.2997762
CGTGCGA1504.6802597E-916.03333310
ATTCGCG957.065872E-515.57894830
CGCGCTT1701.4897523E-915.23529412
CGACGTT750.004106514.824
TAACGGC1406.0038474E-714.53571436
TAGGACA7550.014.4569544
TTAGACT2201.8189894E-1114.2954544
ATTAGAC1703.7371137E-713.0588243
CGCGGAA2152.904926E-912.90697733
TATACTG4450.012.887645
TACGTTA2305.7661964E-1012.86956519
TTAGGAC7050.012.8581553
CGGTCGA2900.012.75862120
CGAATTA3500.012.68571515
TATCAAC61400.012.6245943
TTCGCGG2204.092726E-912.61363631
ATCAACG61650.012.5433914
Produced by FastQC (version 0.11.5)