##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3112168
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85648975248123	34.0	31.0	34.0	31.0	34.0
2	33.07784252006961	34.0	33.0	34.0	31.0	34.0
3	33.20229145727352	34.0	33.0	34.0	31.0	34.0
4	36.49013870716491	37.0	37.0	37.0	35.0	37.0
5	36.4389052904599	37.0	37.0	37.0	35.0	37.0
6	36.485169823737024	37.0	37.0	37.0	35.0	37.0
7	36.4167384280026	37.0	37.0	37.0	35.0	37.0
8	36.42165750692122	37.0	37.0	37.0	35.0	37.0
9	38.20069610637986	39.0	39.0	39.0	37.0	39.0
10	38.17979331449973	39.0	39.0	39.0	37.0	39.0
11	38.21559504499757	39.0	39.0	39.0	37.0	39.0
12	38.15510955706761	39.0	39.0	39.0	37.0	39.0
13	38.166121494726504	39.0	39.0	39.0	37.0	39.0
14	39.627453916369554	41.0	40.0	41.0	37.0	41.0
15	39.61403336837857	41.0	40.0	41.0	37.0	41.0
16	39.532783255916776	41.0	39.0	41.0	37.0	41.0
17	39.46933166847034	41.0	39.0	41.0	37.0	41.0
18	39.36547030880081	41.0	39.0	41.0	36.0	41.0
19	39.39564637898725	41.0	39.0	41.0	36.0	41.0
20	39.60862716922737	41.0	40.0	41.0	37.0	41.0
21	39.61258678837389	41.0	40.0	41.0	37.0	41.0
22	39.62699185905131	41.0	40.0	41.0	37.0	41.0
23	39.55878795746245	41.0	40.0	41.0	37.0	41.0
24	39.52704416985201	41.0	40.0	41.0	37.0	41.0
25	39.49744165482069	41.0	40.0	41.0	37.0	41.0
26	39.36056086946463	41.0	39.0	41.0	36.0	41.0
27	39.279055629387614	41.0	39.0	41.0	36.0	41.0
28	39.24026305777837	41.0	39.0	41.0	36.0	41.0
29	39.13441690808465	41.0	39.0	41.0	36.0	41.0
30	39.04322453029528	41.0	39.0	41.0	35.0	41.0
31	38.91345936337627	40.0	39.0	41.0	35.0	41.0
32	38.82425081165284	40.0	39.0	41.0	35.0	41.0
33	38.74933519013113	40.0	39.0	41.0	35.0	41.0
34	38.67880493598032	40.0	38.0	41.0	35.0	41.0
35	38.548516339734874	40.0	38.0	41.0	35.0	41.0
36	38.5329133902797	40.0	38.0	41.0	35.0	41.0
37	38.48625716863614	40.0	38.0	41.0	35.0	41.0
38	38.38450816279841	40.0	38.0	41.0	35.0	41.0
39	38.32763783960249	40.0	38.0	41.0	35.0	41.0
40	38.26592330491156	40.0	38.0	41.0	34.0	41.0
41	38.16066645502428	40.0	38.0	41.0	34.0	41.0
42	38.06963088110925	40.0	38.0	41.0	34.0	41.0
43	37.76755143038551	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	7.0
13	1.0
14	5.0
15	10.0
16	31.0
17	76.0
18	142.0
19	232.0
20	401.0
21	654.0
22	1150.0
23	1969.0
24	3244.0
25	4870.0
26	6927.0
27	9726.0
28	13338.0
29	17879.0
30	23519.0
31	29997.0
32	39051.0
33	50220.0
34	68325.0
35	95979.0
36	146807.0
37	262748.0
38	562384.0
39	1772475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.207275442713886	19.309304639081184	12.26926052835194	25.21415938985299
2	18.561530097346928	21.57634163708386	35.324089187987276	24.53803907758193
3	18.849528688682614	23.230429719732353	31.52358741558939	26.396454175995643
4	14.171921310160634	16.040425838193826	36.00027376414127	33.78737908750427
5	13.998955069263614	36.69053213065618	35.31544569573365	13.995067104346552
6	33.40719395611033	36.20582179368209	15.597326365414721	14.78965788479285
7	27.988366951912624	31.234432074361024	22.166027026818604	18.61117394690775
8	25.990981206670078	34.51545674912151	20.56408908516507	18.92947295904334
9	25.676473763627154	14.60836947105683	20.63661087704777	39.07854588826824
10	16.05549571874012	27.385378938412064	34.73112634022328	21.827999002624537
11	34.02342032949378	22.39030797823254	22.728785849607092	20.85748584266659
12	20.157652157595603	25.916917081597134	31.125215605327217	22.80021515548004
13	29.337619305898656	20.857614370432444	25.62772960842731	24.17703671524159
14	21.534409453474236	21.9251659936096	26.81751113693091	29.722913415985253
15	24.12520789366127	28.554788816027926	23.59920801190681	23.720795278403994
16	23.21352831852265	27.898044064459242	25.291693764603966	23.596733852414136
17	22.54023561710036	27.429174774626564	27.051399538842375	22.9791900694307
18	22.358593752008247	26.660867922297253	27.828831862547265	23.151706463147235
19	23.54721853061917	26.226733261186414	28.203875883307074	22.022172324887347
20	23.61479200351652	26.13673169314767	27.85813619316181	22.390340110174
21	22.920902727616248	26.406736397263902	27.4332555311924	23.23910534392745
22	23.060612409098738	26.539409183565926	27.40141277720226	22.99856563013308
23	23.01302500379157	26.548406127175657	27.438268114060683	23.0003007549721
24	23.214267353176307	26.466566072268595	27.496683983640985	22.822482590914113
25	23.40124312055133	26.064113505440577	27.52653455726041	23.008108816747683
26	23.125294007264387	26.587092984697485	27.51766614141653	22.769946866621595
27	23.002485726991605	26.706527411116625	27.12572071944702	23.16526614244475
28	22.85255808812378	26.70482441821907	27.441545572089936	23.001071921567213
29	22.72595823875832	26.74164762313603	27.75933047316212	22.77306366494354
30	22.68797828394868	27.14750617575915	27.491350081358075	22.673165458934093
31	23.03474619622077	26.581951874063353	27.561269185982244	22.82203274373363
32	22.428448592749493	26.66221746383871	27.972750828361452	22.936583115050343
33	22.561024983227128	26.53905573220983	27.977249300166314	22.92266998439673
34	22.900177625372407	26.049814791489407	27.772600964986466	23.27740661815172
35	22.62384292878791	26.261435757966794	28.188066968107123	22.926654345138182
36	22.805420529997093	26.01832548885536	27.999259680068683	23.176994301078864
37	22.731549196572935	25.31219394325756	28.466554504769665	23.489702355399835
38	22.34021428149123	25.630300163744373	28.872156001861082	23.157329552903313
39	22.060184411638446	25.76239457510006	29.10491978582133	23.072501227440164
40	21.723088213746816	25.320291192506318	29.600394323185636	23.35622627056123
41	21.425482171913597	25.249118942165076	29.79202922207285	23.53336966384848
42	21.43239053932821	25.258533601013827	29.474244321000665	23.834831538657298
43	21.291491976011578	25.096203032741165	29.778662334424105	23.833642656823155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2935.0
1	2325.0
2	1715.0
3	3156.5
4	4598.0
5	4598.0
6	5114.0
7	5630.0
8	4970.5
9	4311.0
10	5876.5
11	7442.0
12	7442.0
13	11700.5
14	15959.0
15	21336.0
16	26713.0
17	27723.5
18	28734.0
19	28734.0
20	35669.0
21	42604.0
22	44004.0
23	45404.0
24	52988.5
25	60573.0
26	60573.0
27	68374.0
28	76175.0
29	87486.0
30	98797.0
31	109039.5
32	119282.0
33	119282.0
34	128547.0
35	137812.0
36	148807.5
37	159803.0
38	165256.0
39	170709.0
40	170709.0
41	178315.0
42	185921.0
43	184859.5
44	183798.0
45	186752.5
46	189707.0
47	189707.0
48	191342.5
49	192978.0
50	200608.5
51	208239.0
52	215680.5
53	223122.0
54	223122.0
55	208908.5
56	194695.0
57	182985.5
58	171276.0
59	157345.5
60	143415.0
61	143415.0
62	132505.5
63	121596.0
64	107005.5
65	92415.0
66	80281.0
67	68147.0
68	68147.0
69	57658.0
70	47169.0
71	40440.0
72	33711.0
73	27283.5
74	20856.0
75	20856.0
76	16160.0
77	11464.0
78	9352.0
79	7240.0
80	5468.0
81	3696.0
82	3696.0
83	2764.5
84	1833.0
85	1396.5
86	960.0
87	691.5
88	423.0
89	423.0
90	307.0
91	191.0
92	128.5
93	66.0
94	49.0
95	32.0
96	32.0
97	25.5
98	19.0
99	11.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3112168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.63708406219563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21909588977287	45.43375677302398
2	11.754315016147348	13.314602553261315
3	3.549716014327769	6.031366929012117
4	1.513982449574121	3.429902050608734
5	0.8002236354773526	2.2661166655543306
6	0.4657828282304217	1.5828348718328178
7	0.3321637316533007	1.3168949634442408
8	0.21063602223409056	0.9543848078238955
9	0.1491280526771535	0.7601560249956734
>10	0.8383928527577346	8.711138166277657
>50	0.08147691601606172	3.2430815268369404
>100	0.07783033014550976	8.622745237909141
>500	0.00577072724136742	2.2300973412930643
>1k	0.0012569900919437956	0.9826472590295664
>5k	1.1427182654034507E-4	0.43281654118159957
>10k+	1.1427182654034507E-4	0.6874582879149084
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10868	0.3492099398233	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10376	0.33340102462334936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7841	0.2519465530138476	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5534	0.17781816405798145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.426388292662864E-5	0.0	0.0	0.0
6	0.0	6.426388292662864E-5	0.0	0.0	0.0
7	0.0	6.426388292662864E-5	0.0	0.0	0.0
8	0.0	6.426388292662864E-5	0.0	0.0	0.0
9	0.0	6.426388292662864E-5	0.0	0.0	0.0
10	0.0	6.426388292662864E-5	0.0	9.639582438994296E-5	0.0
11	0.0	6.426388292662864E-5	0.0	1.2852776585325727E-4	0.0
12	0.0	6.426388292662864E-5	0.0	3.8558329755977185E-4	0.0
13	0.0	6.426388292662864E-5	0.0	3.8558329755977185E-4	0.0
14	0.0	6.426388292662864E-5	0.0	3.8558329755977185E-4	0.0
15	0.0	6.426388292662864E-5	0.0	3.8558329755977185E-4	0.0
16	0.0	6.426388292662864E-5	0.0	4.1771523902308614E-4	0.0
17	0.0	6.426388292662864E-5	0.0	5.462430048763434E-4	0.0
18	0.0	6.426388292662864E-5	0.0	5.462430048763434E-4	0.0
19	0.0	6.426388292662864E-5	0.0	7.06902712192915E-4	0.0
20	0.0	6.426388292662864E-5	0.0	9.318263024361153E-4	0.0
21	0.0	6.426388292662864E-5	0.0	0.0012531457170692586	0.0
22	0.0	6.426388292662864E-5	0.0	0.0019921803707254877	0.0
23	0.0	6.426388292662864E-5	0.0	0.003084666380478175	0.0
24	0.0	9.639582438994296E-5	0.0	0.0049804509268137195	0.0
25	0.0	9.639582438994296E-5	0.0	0.00899694360972801	0.0
26	0.0	9.639582438994296E-5	0.0	0.01690140120970333	0.0
27	0.0	9.639582438994296E-5	0.0	0.06240023032175641	0.0
28	0.0	9.639582438994296E-5	0.0	0.14816038208734233	0.0
29	0.0	1.2852776585325727E-4	0.0	0.2479621922723966	0.0
30	0.0	1.2852776585325727E-4	0.0	0.3583354111988813	0.0
31	0.0	1.2852776585325727E-4	0.0	0.5778287033347814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2915	0.0	26.655233	1
CGCGAAT	120	5.18412E-9	18.5	28
GTATCAA	4470	0.0	17.299776	2
CGTGCGA	150	4.6802597E-9	16.033333	10
ATTCGCG	95	7.065872E-5	15.578948	30
CGCGCTT	170	1.4897523E-9	15.235294	12
CGACGTT	75	0.0041065	14.8	24
TAACGGC	140	6.0038474E-7	14.535714	36
TAGGACA	755	0.0	14.456954	4
TTAGACT	220	1.8189894E-11	14.295454	4
ATTAGAC	170	3.7371137E-7	13.058824	3
CGCGGAA	215	2.904926E-9	12.906977	33
TATACTG	445	0.0	12.88764	5
TACGTTA	230	5.7661964E-10	12.869565	19
TTAGGAC	705	0.0	12.858155	3
CGGTCGA	290	0.0	12.758621	20
CGAATTA	350	0.0	12.685715	15
TATCAAC	6140	0.0	12.624594	3
TTCGCGG	220	4.092726E-9	12.613636	31
ATCAACG	6165	0.0	12.543391	4
>>END_MODULE