FastQCFastQC Report
Fri 10 Feb 2017
ERR1630036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918348
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.25142974123099304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23040.25088528531667736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17510.19066846119336026No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14150.1540810237513448No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13860.1509231794483137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.1363317609446528No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10670.11618689211497167No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10490.11422685082343512No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA10390.1131379389948037No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10040.10932674759459378No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC9700.10562444737724697No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG9300.10126880006272132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5300.026.1792431
CGACCAA1200.024.66666612
ACCACGA1400.022.4642858
TTTACGC555.1420135E-420.18181833
AACCACG1500.019.7333347
TCGTTGT1254.129106E-1019.2434
CATCGTT1306.9667294E-1018.532
ACGACCA1653.6379788E-1217.93939411
TTCCTCG3200.017.92187519
TACTCCG852.7221511E-517.4117645
ATCTCGC3200.017.3437511
ATAGAAC752.0666963E-417.2666663
CGTTGTA1401.8681021E-917.17857235
GTTGACG650.001579606817.07692336
AATCTCG3250.017.07692310
TTACGCC650.001579606817.07692334
CGGCCTT3500.016.91428624
CCACGAC1757.2759576E-1216.9142869
TCGGCCT3350.016.56716323
TCCTCGG4050.016.44444520
Produced by FastQC (version 0.11.5)