##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630036.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	918348
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.858455618131686	34.0	31.0	34.0	31.0	34.0
2	33.08416308414675	34.0	33.0	34.0	31.0	34.0
3	33.21180859543441	34.0	33.0	34.0	31.0	34.0
4	36.49001141179596	37.0	37.0	37.0	35.0	37.0
5	36.43992909006172	37.0	37.0	37.0	35.0	37.0
6	36.485426004085596	37.0	37.0	37.0	35.0	37.0
7	36.41835339108922	37.0	37.0	37.0	35.0	37.0
8	36.42125425220069	37.0	37.0	37.0	35.0	37.0
9	38.19638415938185	39.0	39.0	39.0	37.0	39.0
10	38.1794581139176	39.0	39.0	39.0	37.0	39.0
11	38.21322418081163	39.0	39.0	39.0	37.0	39.0
12	38.15471041478829	39.0	39.0	39.0	37.0	39.0
13	38.16772291114044	39.0	39.0	39.0	37.0	39.0
14	39.62474682799984	41.0	40.0	41.0	37.0	41.0
15	39.626976919424884	41.0	40.0	41.0	37.0	41.0
16	39.54163127703224	41.0	40.0	41.0	37.0	41.0
17	39.46982843105228	41.0	39.0	41.0	37.0	41.0
18	39.36287006668496	41.0	39.0	41.0	36.0	41.0
19	39.39758457578173	41.0	39.0	41.0	36.0	41.0
20	39.61879265812089	41.0	40.0	41.0	37.0	41.0
21	39.633385165536374	41.0	40.0	41.0	37.0	41.0
22	39.65128796491091	41.0	40.0	41.0	37.0	41.0
23	39.58559609211323	41.0	40.0	41.0	37.0	41.0
24	39.5585845452922	41.0	40.0	41.0	37.0	41.0
25	39.52587363396011	41.0	40.0	41.0	37.0	41.0
26	39.394960298274725	41.0	39.0	41.0	37.0	41.0
27	39.31293257022392	41.0	39.0	41.0	36.0	41.0
28	39.28574679751031	41.0	39.0	41.0	36.0	41.0
29	39.19081001973108	41.0	39.0	41.0	36.0	41.0
30	39.10413372708385	41.0	39.0	41.0	36.0	41.0
31	38.9841748443945	40.0	39.0	41.0	35.0	41.0
32	38.90150139162932	40.0	39.0	41.0	35.0	41.0
33	38.83830094909555	40.0	39.0	41.0	35.0	41.0
34	38.77736653207717	40.0	39.0	41.0	35.0	41.0
35	38.64717841166965	40.0	38.0	41.0	35.0	41.0
36	38.646491308305784	40.0	38.0	41.0	35.0	41.0
37	38.61047990522111	40.0	38.0	41.0	35.0	41.0
38	38.520238515246945	40.0	38.0	41.0	35.0	41.0
39	38.48204928850501	40.0	38.0	41.0	35.0	41.0
40	38.43348708768354	40.0	38.0	41.0	35.0	41.0
41	38.341344457656575	40.0	38.0	41.0	35.0	41.0
42	38.26139219555114	40.0	38.0	41.0	34.0	41.0
43	37.97236015105385	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	4.0
16	2.0
17	20.0
18	17.0
19	41.0
20	78.0
21	169.0
22	326.0
23	545.0
24	812.0
25	1336.0
26	1834.0
27	2767.0
28	3686.0
29	4922.0
30	6661.0
31	8506.0
32	11132.0
33	14436.0
34	19552.0
35	27606.0
36	42159.0
37	74951.0
38	164306.0
39	532475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.810786325009694	19.534533749733214	12.50277672516301	25.151903200094083
2	17.9865366941508	21.689708040960507	36.410489269862836	23.91326599502585
3	18.577271361183342	23.708115006511694	32.03927051618776	25.6753431161172
4	14.311894837251238	16.080832102862967	36.20130930758275	33.405963752303045
5	13.486826344697217	37.4260084412445	35.72959270341962	13.357572510638668
6	33.10912638781813	36.48007073571239	15.819275481625702	14.591527394843784
7	27.02352485114575	31.70737019082091	22.83448104640071	18.434623911632627
8	25.197419714530877	35.59391428957214	20.84874143570847	18.35992456018851
9	25.125442642658342	15.32937404992443	20.694007064859942	38.85117624255729
10	15.790637100532695	27.903256717497072	35.089203657001484	21.21690252496875
11	33.737972968852766	22.792884614546992	23.101700009146857	20.367442407453385
12	19.55544085684294	26.533078963530166	31.191988222329663	22.719491957297233
13	28.72582071284524	21.148845535679285	26.175371427824746	23.949962323650727
14	21.191748661727363	22.216959148383836	26.545165884827977	30.046126305060827
15	23.513090898003806	29.464756279754518	23.96204924494854	23.06010357729314
16	23.23247831976549	28.339801469595404	25.149289811705366	23.27843039893374
17	22.467844433700517	28.168297856585955	26.730389786878177	22.63346792283535
18	21.660089639221734	27.250889640963994	27.98928075195895	23.099739967855324
19	23.208848933084187	26.873255018794616	28.532212189714574	21.38568385840662
20	23.27015466903614	26.512607421151895	28.179731430786585	22.03750647902538
21	22.139319735002417	27.61905073022427	27.356078523609785	22.885551011163525
22	22.627261125412154	26.809771459185406	27.443191469900295	23.11977594550214
23	22.31550566887498	27.356949653072693	27.513099609298436	22.814445068753894
24	23.427066863541928	27.00370665586466	27.416295347733104	22.15293113286031
25	23.27396586043635	26.38694699612783	27.64453126701425	22.694555876421575
26	22.977128495951426	26.898082208487413	27.491974719822988	22.632814575738173
27	22.535683640624253	27.150709752729902	27.215391115350606	23.09821549129524
28	22.236668452482068	27.158223244347457	27.79218771097667	22.81292059219381
29	22.768384098402784	27.508961744349637	27.531393328019444	22.191260829228135
30	22.318445730812282	27.947684320105232	27.301632932178215	22.432237016904267
31	22.800833670896	26.906793503116468	27.794583316999656	22.49778950898788
32	22.077905107867608	27.23477374590025	27.763331547517932	22.92398959871421
33	22.343381811687944	26.89231097579567	27.93995304612195	22.82435416639444
34	22.36189331277468	26.580011063344177	27.94997103494536	23.108124588935787
35	22.697713720724604	26.54505699364511	27.77737851010728	22.979850775523005
36	22.41535888356048	26.592969114104893	28.085867231158556	22.90580477117607
37	22.761415062699545	25.32710911332088	28.245610596418786	23.665865227560793
38	22.090318702714004	25.984376293082796	29.173907930327065	22.751397073876134
39	21.91064825098982	25.749389120464137	29.4961169404191	22.84384568812694
40	21.72444432829385	24.995426570319747	30.035019404408786	23.245109696977618
41	21.040934373461912	25.72445303958848	29.947253111021094	23.287359475928515
42	21.133382987712718	25.04823879400837	29.867871438713866	23.950506779565046
43	20.752481630057453	25.34562061440761	29.96903134759372	23.932866407941216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	598.0
1	496.0
2	394.0
3	728.5
4	1063.0
5	1063.0
6	1220.0
7	1377.0
8	1199.0
9	1021.0
10	1514.0
11	2007.0
12	2007.0
13	3014.0
14	4021.0
15	5627.0
16	7233.0
17	7614.0
18	7995.0
19	7995.0
20	9175.5
21	10356.0
22	11773.5
23	13191.0
24	15043.5
25	16896.0
26	16896.0
27	19192.5
28	21489.0
29	27070.5
30	32652.0
31	34022.0
32	35392.0
33	35392.0
34	38336.5
35	41281.0
36	45025.5
37	48770.0
38	51848.0
39	54926.0
40	54926.0
41	59685.5
42	64445.0
43	61463.0
44	58481.0
45	60761.0
46	63041.0
47	63041.0
48	63759.0
49	64477.0
50	66247.0
51	68017.0
52	69330.5
53	70644.0
54	70644.0
55	61273.0
56	51902.0
57	47596.0
58	43290.0
59	38619.0
60	33948.0
61	33948.0
62	31032.5
63	28117.0
64	25104.5
65	22092.0
66	19574.5
67	17057.0
68	17057.0
69	14463.5
70	11870.0
71	10054.5
72	8239.0
73	6769.5
74	5300.0
75	5300.0
76	4178.5
77	3057.0
78	2482.0
79	1907.0
80	1395.0
81	883.0
82	883.0
83	687.5
84	492.0
85	354.5
86	217.0
87	169.0
88	121.0
89	121.0
90	82.5
91	44.0
92	33.0
93	22.0
94	17.5
95	13.0
96	13.0
97	9.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	918348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.90442401074812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97545897868294	50.643348901990215
2	8.171195974373429	9.626391846988211
3	2.2773478236316835	4.024375854694654
4	1.0055794501421917	2.3693231323068242
5	0.5434421232741498	1.600557262732088
6	0.37085912871315074	1.310714601958563
7	0.2356874372817554	0.9718122917755786
8	0.18045830733362953	0.8503834121153608
9	0.1308599272535052	0.6937405776860512
>10	0.9192212799492299	10.354046499199935
>50	0.09895363126459632	4.0860499043781475
>100	0.08366851684705202	9.69290274545199
>500	0.005403979905870104	2.176419214737485
>1k	0.00186344134685176	1.5999337539849494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2309	0.25142974123099304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2304	0.25088528531667736	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.19066846119336026	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1415	0.1540810237513448	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1386	0.1509231794483137	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.1363317609446528	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1067	0.11618689211497167	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1049	0.11422685082343512	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	1039	0.1131379389948037	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1004	0.10932674759459378	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	970	0.10562444737724697	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	930	0.10126880006272132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	1.088911828631412E-4	0.0	0.0	0.0	0.0
10	1.088911828631412E-4	0.0	0.0	0.0	0.0
11	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
12	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
13	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
14	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
15	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
16	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
17	1.088911828631412E-4	1.088911828631412E-4	0.0	0.0	0.0
18	1.088911828631412E-4	2.177823657262824E-4	0.0	0.0	0.0
19	1.088911828631412E-4	2.177823657262824E-4	0.0	1.088911828631412E-4	0.0
20	1.088911828631412E-4	2.177823657262824E-4	0.0	5.44455914315706E-4	0.0
21	1.088911828631412E-4	2.177823657262824E-4	0.0	7.622382800419884E-4	0.0
22	1.088911828631412E-4	2.177823657262824E-4	0.0	0.001524476560083977	0.0
23	1.088911828631412E-4	2.177823657262824E-4	0.0	0.0027222795715785304	0.0
24	1.088911828631412E-4	2.177823657262824E-4	0.0	0.004682320863115072	0.0
25	1.088911828631412E-4	2.177823657262824E-4	0.0	0.0076223828004198845	0.0
26	1.088911828631412E-4	2.177823657262824E-4	0.0	0.014264744955071499	0.0
27	1.088911828631412E-4	2.177823657262824E-4	0.0	0.059890150574727664	0.0
28	1.088911828631412E-4	2.177823657262824E-4	0.0	0.15418991493420794	0.0
29	1.088911828631412E-4	3.266735485894236E-4	0.0	0.26014103586004433	0.0
30	1.088911828631412E-4	3.266735485894236E-4	0.0	0.3730611924891218	0.0
31	1.088911828631412E-4	3.266735485894236E-4	0.0	0.6413690670639017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	530	0.0	26.179243	1
CGACCAA	120	0.0	24.666666	12
ACCACGA	140	0.0	22.464285	8
TTTACGC	55	5.1420135E-4	20.181818	33
AACCACG	150	0.0	19.733334	7
TCGTTGT	125	4.129106E-10	19.24	34
CATCGTT	130	6.9667294E-10	18.5	32
ACGACCA	165	3.6379788E-12	17.939394	11
TTCCTCG	320	0.0	17.921875	19
TACTCCG	85	2.7221511E-5	17.411764	5
ATCTCGC	320	0.0	17.34375	11
ATAGAAC	75	2.0666963E-4	17.266666	3
CGTTGTA	140	1.8681021E-9	17.178572	35
GTTGACG	65	0.0015796068	17.076923	36
AATCTCG	325	0.0	17.076923	10
TTACGCC	65	0.0015796068	17.076923	34
CGGCCTT	350	0.0	16.914286	24
CCACGAC	175	7.2759576E-12	16.914286	9
TCGGCCT	335	0.0	16.567163	23
TCCTCGG	405	0.0	16.444445	20
>>END_MODULE