FastQCFastQC Report
Fri 10 Feb 2017
ERR1630035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498785
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84620.33864458126649555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.32599843523952643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62800.25132214256128477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.17264390493779977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.029.287841
TCTAGAC6650.020.5864683
GTATCAA30350.019.3838562
TACGCCG701.2199246E-418.535
GCAATTA6100.018.19672217
GTCTAGA6950.017.5683461
TAGAACT7700.017.0584414
ACTGATC6850.016.7445268
GTTCTAG7850.016.4968151
CTCTAGT6550.016.3816827
CTAGACA8150.015.889574
CTAGAAC8400.015.6369063
AACGGAG5850.015.49572725
TAGACAT8850.015.2598875
GCGGAAT1954.1836756E-1115.17948627
CAACGGA5900.015.05084724
ACGGAGT6050.014.67768726
CGGAGTC6050.014.67768727
CGGAATA2400.014.64583428
ACAACGG5950.014.61344623
Produced by FastQC (version 0.11.5)