FastQCFastQC Report
Fri 10 Feb 2017
ERR1630034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666306
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89101.337223437879893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78441.1772368851548687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58780.882177257896522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.7017796627975734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34530.5182303626261807No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32720.49106566652559036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.33288008812767705No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.31742172515330797No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.22917398312487053No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.19750685120650271No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12010.1802475139050226No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9640.14467827094458102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.13747437363613715No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6860.10295569903317696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATAC706.5811037E-926.4285716
ATTGTGG509.0836E-625.9000028
CGTGCGA751.371518E-824.66666610
GTGCGAG851.9736035E-923.94117511
GACACAT3200.023.70312526
CACATGT3150.023.49206428
ACATGTT3100.023.27419529
GTATTAA400.001930339823.1251
CCGTGCG802.72139E-823.1259
TAAGGAT400.001930339823.12526
TAGGCGG400.001930339823.1259
TAGTGCG400.001930339823.12525
CATGTTT3100.022.6774230
TTACGCC1400.022.46428534
ACACATG3300.022.42424227
AGTGACA3550.021.88732523
GTGACAC3550.021.88732524
ACGCCGA1450.021.68965736
TTTACGC1450.021.68965733
GCACCGC3600.021.58333410
Produced by FastQC (version 0.11.5)