FastQCFastQC Report
Fri 10 Feb 2017
ERR1630033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49854
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12952.597584948048301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11002.206442813014001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7571.5184338267741806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.9146708388494403No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.35303085008224017No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.2768082801781201No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.2407028523288001No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.2407028523288001No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.2386969952260601No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.21262085289044008No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA980.19657399606852008No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA920.18453885345208007No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT630.12636899747262004No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT610.12235728326714004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG600.12035142616440005No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.11834556906166005No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.11633971195892005No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACCTGTC580.11633971195892005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.11032214065070005No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA530.10631042644522004No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT520.10430456934248004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG250.00544667529.59999825
CTTATAC851.8644641E-923.94117737
TCTTATA956.7411747E-921.42105336
GGTATCA4950.021.303031
CTCTTAT1601.0146323E-815.03125137
GTATCAA7100.014.8521132
CCCTGTC1000.00179013212.94999932
AACGCAG8300.012.9277117
CGCAGAG8100.012.56172859
AGAGTAC8600.011.83139512
GCAGAGT8600.011.83139510
AAAAAAC1100.003659749911.77272736
CAGAGTA8700.011.69540311
ACGCAGA9250.011.68
TATCAAC9300.011.5376343
TCTCTTA2256.921982E-811.51111137
ACCTGTC1150.005100464411.26086935
GGGAGGC1150.005100464411.26086919
ATCAACG9700.011.2525784
CAACGCA9950.010.969856
Produced by FastQC (version 0.11.5)