##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630033.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49854
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42590363862478	33.0	31.0	34.0	31.0	34.0
2	32.63587676013961	34.0	31.0	34.0	31.0	34.0
3	32.717996549925786	34.0	31.0	34.0	31.0	34.0
4	36.10179724796406	37.0	35.0	37.0	35.0	37.0
5	36.10827616640591	37.0	35.0	37.0	35.0	37.0
6	36.24641954507161	37.0	37.0	37.0	35.0	37.0
7	36.121615116139125	37.0	36.0	37.0	35.0	37.0
8	36.18800898623982	37.0	36.0	37.0	35.0	37.0
9	37.838588678942514	39.0	38.0	39.0	35.0	39.0
10	37.83443655473984	39.0	38.0	39.0	35.0	39.0
11	37.88853452080074	39.0	38.0	39.0	35.0	39.0
12	37.799775344004495	39.0	38.0	39.0	35.0	39.0
13	37.8382878003771	39.0	38.0	39.0	35.0	39.0
14	39.00082240141212	40.0	38.0	41.0	36.0	41.0
15	39.004192241344725	40.0	38.0	41.0	36.0	41.0
16	38.85852689854375	40.0	38.0	41.0	35.0	41.0
17	38.94792794961287	40.0	38.0	41.0	36.0	41.0
18	38.889457215068	40.0	38.0	41.0	35.0	41.0
19	38.92875195571067	40.0	39.0	41.0	35.0	41.0
20	39.140771051470296	40.0	39.0	41.0	36.0	41.0
21	39.100613792273435	40.0	39.0	41.0	35.0	41.0
22	39.03411962931761	40.0	39.0	41.0	35.0	41.0
23	38.908512857544025	40.0	38.0	41.0	35.0	41.0
24	38.835178721867855	40.0	38.0	41.0	35.0	41.0
25	38.753038873510654	40.0	38.0	41.0	35.0	41.0
26	38.46437597785534	40.0	38.0	41.0	35.0	41.0
27	38.20403578449071	40.0	37.0	41.0	34.0	41.0
28	38.03245476792233	40.0	37.0	41.0	34.0	41.0
29	37.78011794439764	40.0	36.0	41.0	33.0	41.0
30	37.48254904320616	40.0	36.0	41.0	33.0	41.0
31	37.12887631885104	39.0	35.0	41.0	33.0	41.0
32	36.85517711718217	39.0	35.0	41.0	33.0	41.0
33	36.57327395996309	39.0	35.0	41.0	32.0	41.0
34	36.311750310907854	38.0	35.0	41.0	31.0	41.0
35	35.98780438881534	38.0	35.0	40.0	30.0	41.0
36	35.728447065431055	38.0	35.0	40.0	29.0	41.0
37	35.44840935531753	38.0	35.0	40.0	27.0	41.0
38	35.120933124724196	38.0	35.0	40.0	25.0	41.0
39	34.78607533999278	37.0	35.0	40.0	23.0	41.0
40	34.40131183054519	37.0	35.0	40.0	21.0	41.0
41	33.89808240060978	37.0	34.0	40.0	18.0	41.0
42	33.4704938420187	37.0	33.0	40.0	15.0	41.0
43	32.88137361094395	36.0	33.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	4.0
19	3.0
20	11.0
21	31.0
22	57.0
23	84.0
24	144.0
25	206.0
26	312.0
27	437.0
28	623.0
29	777.0
30	943.0
31	1134.0
32	1301.0
33	1712.0
34	2209.0
35	2908.0
36	4188.0
37	8801.0
38	10166.0
39	13800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.504553295623225	17.015685802543427	13.812332009467646	19.667428892365706
2	21.93805913266739	23.93789866409917	31.39567537208649	22.72836683114695
3	23.87571709391423	23.76940666746901	30.69563124323023	21.65924499538653
4	15.019857985317126	16.750912664981747	30.583303245476788	37.64592610422434
5	14.458217996549926	38.16143137962851	31.33349380190155	16.046856821920006
6	33.650258755566256	32.296305211216755	13.252697877803184	20.800738155413807
7	27.482248164640747	30.99851566574397	20.160869739639747	21.35836642997553
8	30.041721827736996	31.51602679825089	17.300517511132508	21.141733862879608
9	25.78328719861997	13.451277730974445	19.14590604565331	41.619529024752275
10	21.05748786456453	27.16933445661331	26.34893890159265	25.424238777229508
11	35.5879167168131	22.244955269386608	19.169976330886186	22.99715168291411
12	22.32117783929073	29.273478557387573	26.044048621976167	22.361294981345527
13	34.651181449833516	19.067677618646446	23.26593653468127	23.01520439683877
14	21.388454286516627	23.66911381233201	27.732980302483252	27.20945159866811
15	26.29478075981867	27.64472258996269	23.54876238616761	22.51173426405103
16	21.390460143619368	26.05407790748987	27.391984595017448	25.163477353873308
17	22.26300798331127	27.496289164359933	26.61972961046255	23.62097324186625
18	22.776507401612708	23.36221767561279	28.362819432743613	25.498455490030892
19	24.477474224736227	24.61788422192803	30.39475267781923	20.50988887551651
20	23.71123681148955	25.125366068921252	29.766919404661614	21.396477714927588
21	23.245877963653868	25.33397520760621	30.545191960524733	20.874954868215188
22	22.54583383479761	26.36097404420909	30.244313395113732	20.84887872587957
23	21.92000641874273	26.11024190636659	30.44690496249047	21.52284671240021
24	21.909977133229027	26.12227704898303	30.73976009949051	21.22798571829743
25	20.893007582139848	26.31483933084607	30.64347895855899	22.148674128455088
26	21.105628435030287	27.103141172222887	31.34552894451799	20.445701448228828
27	21.35836642997553	26.95069603241465	31.13691980583303	20.55401773177679
28	20.43567216271513	28.055923296024392	31.036626950696032	20.47177759056445
29	20.97324186624945	28.310667148072373	30.366670678380874	20.349420307297308
30	19.98435431459863	28.579451999839534	30.982468808922054	20.45372487663979
31	20.35142616440005	28.18630400770249	30.96040438079191	20.50186544710555
32	20.517912303927467	27.783126730051748	31.14895494844947	20.55000601757131
33	19.87002045974245	28.47314157339431	30.667549243791868	20.98928872307137
34	20.33337345047539	28.597504713764195	30.284430537168532	20.78469129859189
35	19.408673326112247	28.663697998154614	30.77385967023709	21.15376900549605
36	19.619288321899948	27.75303887351065	30.962410237894648	21.66526256669475
37	19.52100132386569	27.78914430135997	31.042644522004252	21.64720985277009
38	18.800898623982025	27.56248244875035	31.848999077305734	21.78761984996189
39	18.65447105548201	27.71492758855859	32.30432863962771	21.326272716331687
40	18.85706262285875	27.291691739880452	32.46680306494965	21.384442572311148
41	18.17306535082441	27.869378585469573	32.468808922052396	21.488747141653626
42	17.96846792634493	27.32579131062703	31.93123921851807	22.77450154450997
43	17.846110643077786	26.884502748024232	32.35848678140169	22.91089982749629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	88.0
2	71.0
3	144.5
4	218.0
5	218.0
6	286.0
7	354.0
8	331.0
9	308.0
10	407.5
11	507.0
12	507.0
13	1001.5
14	1496.0
15	2164.5
16	2833.0
17	2407.0
18	1981.0
19	1981.0
20	2121.5
21	2262.0
22	1553.0
23	844.0
24	756.0
25	668.0
26	668.0
27	652.0
28	636.0
29	705.0
30	774.0
31	841.5
32	909.0
33	909.0
34	902.0
35	895.0
36	977.0
37	1059.0
38	1145.5
39	1232.0
40	1232.0
41	1311.5
42	1391.0
43	1467.5
44	1544.0
45	1741.5
46	1939.0
47	1939.0
48	2261.0
49	2583.0
50	2739.5
51	2896.0
52	2934.0
53	2972.0
54	2972.0
55	3017.5
56	3063.0
57	3206.0
58	3349.0
59	3081.5
60	2814.0
61	2814.0
62	2650.5
63	2487.0
64	2332.0
65	2177.0
66	2007.5
67	1838.0
68	1838.0
69	1643.0
70	1448.0
71	1207.5
72	967.0
73	781.5
74	596.0
75	596.0
76	462.0
77	328.0
78	242.5
79	157.0
80	126.0
81	95.0
82	95.0
83	64.0
84	33.0
85	24.0
86	15.0
87	10.0
88	5.0
89	5.0
90	4.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	49854.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.97416456051671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.87971684967393	63.25069202070045
2	8.279917507385319	11.918802904481085
3	1.9257566467866898	4.158141773980022
4	0.7441056797279973	2.1422553857263207
5	0.3316426063207179	1.1934849761303004
6	0.15885402151496572	0.6860031291370803
7	0.09475503037734798	0.4773939904521202
8	0.07524664177024692	0.43326513419184015
9	0.06688590379577504	0.43326513419184015
>10	0.38459394682570647	5.084847755445902
>50	0.030656039239730225	1.4221526858426605
>100	0.019508388607101055	2.477233521883901
>500	0.0027869126581572933	1.5184338267741806
>1k	0.005573825316314587	4.804027761062302
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1295	2.597584948048301	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1100	2.206442813014001	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	757	1.5184338267741806	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.9146708388494403	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.35303085008224017	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	138	0.2768082801781201	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.2407028523288001	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.2407028523288001	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119	0.2386969952260601	No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106	0.21262085289044008	No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA	98	0.19657399606852008	No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA	92	0.18453885345208007	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	63	0.12636899747262004	No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT	61	0.12235728326714004	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	60	0.12035142616440005	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.11834556906166005	No Hit
CAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.11633971195892005	No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACCTGTC	58	0.11633971195892005	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.11032214065070005	No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA	53	0.10631042644522004	No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT	52	0.10430456934248004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0020058571027400007	0.0
2	0.0	0.0	0.0	0.0020058571027400007	0.0
3	0.0	0.0	0.0	0.0020058571027400007	0.0
4	0.0	0.0	0.0	0.0020058571027400007	0.0
5	0.0	0.0	0.0	0.0020058571027400007	0.0
6	0.0	0.0	0.0	0.0020058571027400007	0.0
7	0.0	0.0	0.0	0.0020058571027400007	0.0
8	0.0	0.0	0.0	0.0020058571027400007	0.0
9	0.0	0.0	0.0	0.0020058571027400007	0.0
10	0.0	0.0	0.0	0.0020058571027400007	0.0
11	0.0	0.0	0.0	0.0020058571027400007	0.0
12	0.0	0.0	0.0	0.0020058571027400007	0.0
13	0.0	0.0	0.0	0.0040117142054800015	0.0
14	0.0	0.0	0.0	0.0040117142054800015	0.0
15	0.0	0.0	0.0	0.0040117142054800015	0.0
16	0.0	0.0	0.0	0.0040117142054800015	0.0
17	0.0	0.0	0.0	0.0040117142054800015	0.0
18	0.0	0.0	0.0	0.0040117142054800015	0.0
19	0.0	0.0	0.0	0.0040117142054800015	0.0
20	0.0	0.0	0.0	0.006017571308220003	0.0
21	0.0	0.0	0.0	0.010029285513700003	0.0
22	0.0	0.0	0.0	0.010029285513700003	0.0
23	0.0	0.0	0.0	0.014040999719180006	0.0
24	0.0	0.0	0.0	0.018052713924660006	0.0
25	0.0	0.0	0.0	0.03209371364384001	0.0
26	0.0	0.0	0.0	0.04212299915754002	0.0
27	0.0	0.0	0.0	0.14442171139728005	0.0
28	0.0	0.0	0.0	0.3249488506438801	0.0
29	0.0	0.0	0.0	0.6258274160548802	0.0
30	0.0	0.0	0.0	0.9768524090343804	0.0
31	0.0	0.0	0.0	1.4020941148152606	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGCG	25	0.005446675	29.599998	25
CTTATAC	85	1.8644641E-9	23.941177	37
TCTTATA	95	6.7411747E-9	21.421053	36
GGTATCA	495	0.0	21.30303	1
CTCTTAT	160	1.0146323E-8	15.031251	37
GTATCAA	710	0.0	14.852113	2
CCCTGTC	100	0.001790132	12.949999	32
AACGCAG	830	0.0	12.927711	7
CGCAGAG	810	0.0	12.5617285	9
AGAGTAC	860	0.0	11.831395	12
GCAGAGT	860	0.0	11.831395	10
AAAAAAC	110	0.0036597499	11.772727	36
CAGAGTA	870	0.0	11.695403	11
ACGCAGA	925	0.0	11.6	8
TATCAAC	930	0.0	11.537634	3
TCTCTTA	225	6.921982E-8	11.511111	37
ACCTGTC	115	0.0051004644	11.260869	35
GGGAGGC	115	0.0051004644	11.260869	19
ATCAACG	970	0.0	11.252578	4
CAACGCA	995	0.0	10.96985	6
>>END_MODULE