FastQCFastQC Report
Fri 10 Feb 2017
ERR1630032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710247
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109740.29577545645882874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.2723538352028854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79980.21556516318185825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58220.15691677669977228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.028.2037731
GTATCAA45450.016.3223322
CGAATTA3600.015.41666715
ATTCGCG1203.3042576E-513.87499930
GTATTAG6100.013.6475411
TAACGCT3150.013.50793732
CTTATAC36050.013.49653237
AACGCTA3200.013.29687533
GTATAGG4750.013.24210551
GTACGGT850.00940989413.05882411
CGCGCTT1857.397466E-813.012
CTTAACG3450.012.86956530
ACGTTAA3600.012.84722220
TTACACT7050.012.5957454
AATCTCG6850.012.42335710
TTAGGAC12000.012.3333333
CTTACAC6350.012.236223
CCGAATT5000.012.21000114
TACGTTA3500.012.15714319
CTCTTAT72650.012.04473537
Produced by FastQC (version 0.11.5)