FastQCFastQC Report
Fri 10 Feb 2017
ERR1630031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282703
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.8705248971535499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24090.8521310350438447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16580.5864812188056017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.39157702606622496No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATT6150.21754279225901385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.16271493404739248No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.15387173111003422No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.13653905335281197No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3340.11814519124310671No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.11390045383317475No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC2930.10364233842583913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCAT250.00548942229.632
TTCAAGG250.00548942229.62
GTTGGCA400.001927606523.12531
GACTACA1550.022.6774181
GGTATCA6450.022.3720931
TGTATAG502.6951244E-422.23
GACAGGC855.145921E-821.7647067
AACCACG1550.021.483877
GTAAACA1059.731593E-1021.14285736
TTGTATA450.003818781620.5555572
CCGAGTT450.003818781620.55555713
TCTCGAG450.003818781620.55555730
TGCCGAG450.003818781620.55555711
GACGGTT555.129919E-420.18181811
ACGGTTT555.129919E-420.18181812
ACTACAA1750.020.0857142
ACCACGA1700.019.5882368
CCACGAC1700.019.5882369
CATCAGA851.2385244E-619.58823616
AGGACAG1052.2413587E-819.380955
Produced by FastQC (version 0.11.5)