##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630031.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282703
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66853906750194	33.0	31.0	34.0	31.0	34.0
2	32.90900344177458	34.0	31.0	34.0	31.0	34.0
3	33.02268104689374	34.0	33.0	34.0	31.0	34.0
4	36.3369614047251	37.0	37.0	37.0	35.0	37.0
5	36.312285331248695	37.0	37.0	37.0	35.0	37.0
6	36.39911497225003	37.0	37.0	37.0	35.0	37.0
7	36.31763723766638	37.0	37.0	37.0	35.0	37.0
8	36.33920050370884	37.0	37.0	37.0	35.0	37.0
9	38.10120161441513	39.0	39.0	39.0	37.0	39.0
10	38.07593481498251	39.0	39.0	39.0	37.0	39.0
11	38.11419758545187	39.0	39.0	39.0	37.0	39.0
12	38.0373112418333	39.0	38.0	39.0	35.0	39.0
13	38.06689352429935	39.0	38.0	39.0	35.0	39.0
14	39.44488385337262	41.0	39.0	41.0	37.0	41.0
15	39.416429963601374	41.0	39.0	41.0	36.0	41.0
16	39.31980205374545	41.0	39.0	41.0	36.0	41.0
17	39.28986250588073	41.0	39.0	41.0	36.0	41.0
18	39.216163252600786	40.0	39.0	41.0	36.0	41.0
19	39.261560719200006	41.0	39.0	41.0	36.0	41.0
20	39.45446634807554	41.0	39.0	41.0	37.0	41.0
21	39.445156224023094	41.0	39.0	41.0	37.0	41.0
22	39.45872523461017	41.0	39.0	41.0	37.0	41.0
23	39.38154883393526	41.0	39.0	41.0	36.0	41.0
24	39.34723720653831	41.0	39.0	41.0	36.0	41.0
25	39.303979795049926	41.0	39.0	41.0	36.0	41.0
26	39.1218451873521	41.0	39.0	41.0	35.0	41.0
27	39.02907291397686	41.0	39.0	41.0	35.0	41.0
28	38.96318751481237	40.0	39.0	41.0	35.0	41.0
29	38.83683583124339	40.0	39.0	41.0	35.0	41.0
30	38.68735386607146	40.0	38.0	41.0	35.0	41.0
31	38.51118665171576	40.0	38.0	41.0	35.0	41.0
32	38.388793185781545	40.0	38.0	41.0	35.0	41.0
33	38.27246615706236	40.0	38.0	41.0	35.0	41.0
34	38.18279961655872	40.0	38.0	41.0	34.0	41.0
35	37.989844465746735	40.0	38.0	41.0	34.0	41.0
36	37.933049879201846	40.0	38.0	41.0	34.0	41.0
37	37.83888391704368	40.0	37.0	41.0	33.0	41.0
38	37.70414533980892	40.0	37.0	41.0	33.0	41.0
39	37.62915497890012	40.0	37.0	41.0	33.0	41.0
40	37.49478074162637	40.0	37.0	41.0	33.0	41.0
41	37.324297938118804	40.0	37.0	41.0	33.0	41.0
42	37.163086348570765	40.0	37.0	41.0	32.0	41.0
43	36.791233202336024	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	2.0
17	7.0
18	9.0
19	18.0
20	37.0
21	68.0
22	123.0
23	192.0
24	374.0
25	569.0
26	795.0
27	1197.0
28	1540.0
29	2161.0
30	2697.0
31	3454.0
32	4254.0
33	5581.0
34	7910.0
35	10942.0
36	16400.0
37	31480.0
38	58238.0
39	134653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99175106030003	19.366968160932142	12.855894702213986	23.785386076553838
2	19.833181819789672	21.8409426146875	34.74883535017315	23.57704021534968
3	18.97291503804346	25.18933297488884	30.505159124593657	25.332592862474044
4	13.376582491165639	16.943930556095975	37.77816294839461	31.901324004343785
5	12.642950375482398	38.42690031587921	35.7314920605724	13.198657248065992
6	30.606679094314526	38.13684325953386	15.321733409266969	15.934744236884645
7	27.58230368973799	30.332186075138928	23.815806694658352	18.269703540464725
8	26.11150217719656	34.09549951716112	19.830705722967213	19.962292582675104
9	25.19605380912123	14.706600212944327	20.781173174674482	39.31617280325996
10	18.031644517391044	26.52536407466493	33.29996498091637	22.143026427027657
11	32.89034782085793	24.384247779471742	22.071573347293803	20.65383105237652
12	20.382167858140875	28.736164809004507	28.985896859955503	21.89577047289912
13	29.717052878816286	20.746507819160037	25.44401721948476	24.09242208253892
14	21.362702199835162	22.448293792425268	27.481844904369602	28.707159103369968
15	24.371159839124452	28.861384562597497	24.225777582834283	22.541678015443768
16	21.98809351156514	26.697275939767177	27.040745941854173	24.27388460681351
17	21.945292409348326	28.334683395648437	27.197447497904157	22.522576697099076
18	21.322377194440808	26.984503171172573	28.150037318316397	23.543082316070223
19	23.124975681191923	25.805527355563967	30.261086723522567	20.808410239721546
20	22.583771661425594	26.245565133726917	29.64418488661245	21.526478318235036
21	21.54947064587217	27.36546835371397	29.158869909410228	21.926191091003634
22	21.93892530323343	26.573824826761655	29.618362734035365	21.86888713596955
23	21.756401594606352	27.427370774275477	29.404003494833802	21.41222413628437
24	21.00260697622593	27.995104402853876	28.334329667530945	22.66795895338925
25	22.019575314022134	26.632897422383206	29.689815813769222	21.657711449825435
26	20.934337449549528	27.989798481091466	29.587234659695866	21.488629409663147
27	21.15753989168845	28.037551776953194	28.516853376158018	22.288054955200334
28	21.004021888695913	27.602112464317674	30.312377300559245	21.081488346427168
29	22.007902286144823	27.748909633077822	29.512598026904563	20.730590053872792
30	21.188667966027953	27.806213588111905	29.113946438488448	21.891172007371694
31	21.246679377297024	27.540917499991156	29.85465311652158	21.357750006190244
32	20.77515979667708	27.075057569251122	29.782138852435242	22.36764378163656
33	20.568936304177882	28.115371962801948	29.156747540705265	22.1589441923149
34	21.692023077222387	26.254762064781765	30.12419394205226	21.929020915943585
35	21.309289254093517	27.027304273389387	29.659395195664708	22.00401127685239
36	21.92053144112372	27.286940711630226	28.498105785930818	22.29442206131523
37	20.63897447144176	25.71178940442797	30.783543153061693	22.865692971068576
38	20.93080016837458	26.178003063285498	30.89072277266248	22.000473995677442
39	20.196814324573847	26.286243867238763	30.950502824519017	22.566438983668373
40	20.219452924093485	25.788194677806743	31.66043515633014	22.33191724176963
41	19.81089694838753	25.539170083090735	31.478265175820564	23.17166779270117
42	19.058870970594583	26.410402436479274	30.908055450419695	23.622671142506448
43	19.80346865792015	24.88229696890376	31.900262819991298	23.41397155318479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	276.0
2	268.0
3	524.0
4	780.0
5	780.0
6	850.5
7	921.0
8	818.5
9	716.0
10	1011.0
11	1306.0
12	1306.0
13	2176.5
14	3047.0
15	4118.0
16	5189.0
17	4580.5
18	3972.0
19	3972.0
20	4217.5
21	4463.0
22	3219.0
23	1975.0
24	2051.0
25	2127.0
26	2127.0
27	2710.0
28	3293.0
29	4339.5
30	5386.0
31	6954.0
32	8522.0
33	8522.0
34	10121.0
35	11720.0
36	13910.0
37	16100.0
38	17356.5
39	18613.0
40	18613.0
41	20801.0
42	22989.0
43	24503.0
44	26017.0
45	25516.0
46	25015.0
47	25015.0
48	24021.0
49	23027.0
50	22570.5
51	22114.0
52	20090.5
53	18067.0
54	18067.0
55	16944.5
56	15822.0
57	13047.0
58	10272.0
59	9079.0
60	7886.0
61	7886.0
62	7369.5
63	6853.0
64	5853.5
65	4854.0
66	4092.5
67	3331.0
68	3331.0
69	2920.0
70	2509.0
71	2169.5
72	1830.0
73	1603.0
74	1376.0
75	1376.0
76	1229.0
77	1082.0
78	797.5
79	513.0
80	364.0
81	215.0
82	215.0
83	166.5
84	118.0
85	92.0
86	66.0
87	52.5
88	39.0
89	39.0
90	28.0
91	17.0
92	12.0
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	282703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.95157707400607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95126801813406	28.06567247910694
2	11.37244488683682	8.632044372853475
3	4.023346841283201	4.580770732272548
4	2.0995424855944202	3.1872379384874763
5	1.3942983325502243	2.6457910315968984
6	1.0136619653926255	2.3082042123952022
7	0.7776442375175804	2.0658977651413593
8	0.6312716442149608	1.9166203568046893
9	0.5088003245751596	1.7378797260054122
>10	3.8125164226633013	28.33774760763013
>50	0.32459659051193196	8.344767747251533
>100	0.08562977541112315	5.0598397018384285
>500	9.956950629200368E-4	0.2323974172240951
>1k	0.003982780251680147	2.8851289113918153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2461	0.8705248971535499	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2409	0.8521310350438447	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1658	0.5864812188056017	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.39157702606622496	No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATT	615	0.21754279225901385	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.16271493404739248	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.15387173111003422	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.13653905335281197	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	334	0.11814519124310671	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.11390045383317475	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	293	0.10364233842583913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.537281174943315E-4	0.0
2	0.0	0.0	0.0	3.537281174943315E-4	0.0
3	0.0	0.0	0.0	3.537281174943315E-4	0.0
4	0.0	0.0	0.0	3.537281174943315E-4	0.0
5	0.0	0.0	0.0	3.537281174943315E-4	0.0
6	0.0	0.0	0.0	3.537281174943315E-4	0.0
7	0.0	0.0	0.0	3.537281174943315E-4	0.0
8	0.0	0.0	0.0	3.537281174943315E-4	0.0
9	0.0	0.0	0.0	0.0010611843524829946	0.0
10	0.0	0.0	0.0	0.0010611843524829946	0.0
11	0.0	0.0	0.0	0.0010611843524829946	0.0
12	0.0	0.0	0.0	0.0010611843524829946	0.0
13	0.0	0.0	0.0	0.0010611843524829946	0.0
14	0.0	0.0	0.0	0.0010611843524829946	0.0
15	0.0	0.0	0.0	0.0010611843524829946	0.0
16	0.0	0.0	0.0	0.0010611843524829946	0.0
17	0.0	0.0	0.0	0.0010611843524829946	0.0
18	0.0	0.0	0.0	0.0010611843524829946	0.0
19	0.0	0.0	0.0	0.0010611843524829946	0.0
20	0.0	0.0	0.0	0.0010611843524829946	0.0
21	0.0	0.0	0.0	0.0017686405874716576	0.0
22	0.0	0.0	0.0	0.002122368704965989	0.0
23	0.0	0.0	0.0	0.004952193644920641	0.0
24	0.0	0.0	0.0	0.010965571642324277	0.0
25	0.0	0.0	0.0	0.01627149340473925	0.0
26	0.0	0.0	0.0	0.03077434622200684	0.0
27	0.0	0.0	0.0	0.10647216336579378	0.0
28	0.0	0.0	0.0	0.23664411060370777	0.0
29	0.0	0.0	0.0	0.4255349253456808	0.0
30	0.0	0.0	0.0	0.6161943806751254	0.0
31	0.0	0.0	0.0	0.9982207475690035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCAT	25	0.005489422	29.6	32
TTCAAGG	25	0.005489422	29.6	2
GTTGGCA	40	0.0019276065	23.125	31
GACTACA	155	0.0	22.677418	1
GGTATCA	645	0.0	22.372093	1
TGTATAG	50	2.6951244E-4	22.2	3
GACAGGC	85	5.145921E-8	21.764706	7
AACCACG	155	0.0	21.48387	7
GTAAACA	105	9.731593E-10	21.142857	36
TTGTATA	45	0.0038187816	20.555557	2
CCGAGTT	45	0.0038187816	20.555557	13
TCTCGAG	45	0.0038187816	20.555557	30
TGCCGAG	45	0.0038187816	20.555557	11
GACGGTT	55	5.129919E-4	20.181818	11
ACGGTTT	55	5.129919E-4	20.181818	12
ACTACAA	175	0.0	20.085714	2
ACCACGA	170	0.0	19.588236	8
CCACGAC	170	0.0	19.588236	9
CATCAGA	85	1.2385244E-6	19.588236	16
AGGACAG	105	2.2413587E-8	19.38095	5
>>END_MODULE