FastQCFastQC Report
Fri 10 Feb 2017
ERR1630030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4629946
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121710.2628756361305294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108990.23540231354750143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91590.1978208817122273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65250.1409303693822779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.028.7550661
CGAATTA5050.016.4851515
GTATCAA48650.016.009252
TGTACCG2003.6379788E-1215.7255
TAGGACA17550.014.3361834
TTAACGG2353.6379788E-1214.17021435
CGAGCGT3450.013.9420286
CTTAGGA18250.013.8876722
GTATTAG8400.013.8751
CCGAATT6500.013.66153814
GTACCGA1501.3078316E-613.5666666
TTAGGAC18150.013.2506893
GCTTAGG17800.012.9915721
AATCTCG9950.012.82914510
GCTACGA4100.012.6341472
TAACGGC2805.456968E-1212.55357236
CGACGTT1359.9726494E-512.33333424
GTTTAAG10500.012.3333341
CTTAGAC3600.012.3333343
ATAAGCG1050.002645654412.3333335
Produced by FastQC (version 0.11.5)