##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630030.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4629946
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.851826349594575	34.0	31.0	34.0	31.0	34.0
2	33.07774971889521	34.0	33.0	34.0	31.0	34.0
3	33.201913802018424	34.0	33.0	34.0	31.0	34.0
4	36.48731389091795	37.0	37.0	37.0	35.0	37.0
5	36.43807897543513	37.0	37.0	37.0	35.0	37.0
6	36.48314105607279	37.0	37.0	37.0	35.0	37.0
7	36.41729925143835	37.0	37.0	37.0	35.0	37.0
8	36.42192371142126	37.0	37.0	37.0	35.0	37.0
9	38.19556556383163	39.0	39.0	39.0	37.0	39.0
10	38.176646768666416	39.0	39.0	39.0	37.0	39.0
11	38.2058639560807	39.0	39.0	39.0	37.0	39.0
12	38.14654252986968	39.0	39.0	39.0	37.0	39.0
13	38.156627096730716	39.0	39.0	39.0	37.0	39.0
14	39.62284419731893	41.0	40.0	41.0	37.0	41.0
15	39.61403394337645	41.0	40.0	41.0	37.0	41.0
16	39.535593719667574	41.0	39.0	41.0	37.0	41.0
17	39.46400800354907	41.0	39.0	41.0	37.0	41.0
18	39.35812469519083	41.0	39.0	41.0	36.0	41.0
19	39.38772612034784	41.0	39.0	41.0	36.0	41.0
20	39.60744704149897	41.0	40.0	41.0	37.0	41.0
21	39.61543611955734	41.0	40.0	41.0	37.0	41.0
22	39.63666962854426	41.0	40.0	41.0	37.0	41.0
23	39.574385100819754	41.0	40.0	41.0	37.0	41.0
24	39.5451910238262	41.0	40.0	41.0	37.0	41.0
25	39.519484892480385	41.0	40.0	41.0	37.0	41.0
26	39.38758335410392	41.0	39.0	41.0	37.0	41.0
27	39.31256692842638	41.0	39.0	41.0	36.0	41.0
28	39.28142898426893	41.0	39.0	41.0	36.0	41.0
29	39.18269241153136	41.0	39.0	41.0	36.0	41.0
30	39.10494010081327	41.0	39.0	41.0	36.0	41.0
31	38.9832922457411	40.0	39.0	41.0	35.0	41.0
32	38.902176828844226	40.0	39.0	41.0	35.0	41.0
33	38.83421836885355	40.0	39.0	41.0	35.0	41.0
34	38.767761438254354	40.0	39.0	41.0	35.0	41.0
35	38.64106600811327	40.0	38.0	41.0	35.0	41.0
36	38.63218253517427	40.0	38.0	41.0	35.0	41.0
37	38.59231576350999	40.0	38.0	41.0	35.0	41.0
38	38.5011356503942	40.0	38.0	41.0	35.0	41.0
39	38.46095397224935	40.0	38.0	41.0	35.0	41.0
40	38.406685736723496	40.0	38.0	41.0	35.0	41.0
41	38.306370743848845	40.0	38.0	41.0	34.0	41.0
42	38.220910567855434	40.0	38.0	41.0	34.0	41.0
43	37.92929809548534	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	2.0
14	4.0
15	12.0
16	25.0
17	81.0
18	167.0
19	289.0
20	505.0
21	967.0
22	1668.0
23	2919.0
24	4472.0
25	6771.0
26	9816.0
27	13758.0
28	18938.0
29	25207.0
30	33580.0
31	43483.0
32	55674.0
33	73155.0
34	99121.0
35	140110.0
36	212731.0
37	375456.0
38	833345.0
39	2677685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09721970839401	19.367331714019993	12.155022974350024	25.380425603235977
2	18.240795896971584	21.576515147260896	35.821087330176205	24.361601625591316
3	18.671297678201864	23.5334710167246	31.604040306301627	26.19119099877191
4	14.126860226879536	16.01379368139499	36.24070345528868	33.618642636436796
5	13.711607003623799	37.07267860143509	35.566267943513814	13.649446451427295
6	33.28464306063181	36.47070613782537	15.748455813523526	14.496194988019298
7	27.686456818286864	31.32492258009057	22.520521837619704	18.468098764002864
8	25.674359916940716	35.07939401453062	20.67000349464119	18.576242573887473
9	25.548440521768505	14.821166380774203	20.49447228974161	39.135920807715685
10	15.801091416616956	27.53522395293595	35.016391119896426	21.647293510550664
11	33.992642678769904	22.514776630224198	22.937654132467202	20.554926558538696
12	19.94116130080135	26.05475312239063	31.168808448305878	22.835277128502145
13	28.974096026173957	20.93195039423786	26.008834660274655	24.08511891931353
14	21.455671405238853	21.81258701505374	26.563527954753685	30.168213624953726
15	23.84919824118899	28.87947289234043	23.696734259967613	23.57459460650297
16	23.42975490426886	27.945444720089608	25.12869480551177	23.49610557012976
17	22.63892494642486	27.64317337610417	26.75158630359836	22.96631537387261
18	22.242289650894417	26.769167502169573	27.755226518840608	23.233316328095402
19	23.51379476132119	26.49646453759936	28.10611614044743	21.883624560632025
20	23.51517706685996	26.248167905198027	27.902442058719473	22.334212969222538
21	22.66488637232486	26.855863977679224	27.21100850852256	23.268241141473357
22	22.979663261731346	26.571778590938212	27.24025291007714	23.208305237253306
23	22.824197085667954	26.775863044622984	27.34759757457215	23.052342295136917
24	23.4310724142355	26.6246949748442	27.231159931454922	22.713072679465377
25	23.48200173392951	26.06073591355061	27.4714435114362	22.985818841083674
26	23.145691116051896	26.63892840218871	27.308374654909585	22.907005826849815
27	22.96856162037311	26.734350681411833	26.97977471011541	23.317312988099644
28	22.735664735614627	26.772709660112664	27.384228671349515	23.10739693292319
29	22.912081479999983	26.974072699767987	27.42414274378146	22.689703076450566
30	22.720070601255394	27.362738139926467	27.18290882874228	22.734282430075858
31	23.12182474698409	26.59132525519736	27.520947328543354	22.765902669275192
32	22.42306929713651	26.898089092183795	27.671618632269144	23.007222978410546
33	22.61302831609699	26.619122555641038	27.781965491606165	22.985883636655803
34	22.85761000236288	26.272423047698613	27.590667364155003	23.279299585783505
35	22.811086781573696	26.354043870058096	27.843175708744766	22.991693639623442
36	22.80668068266887	26.17836147549021	27.78107995212039	23.233877889720528
37	22.878107001679933	25.194095136314765	28.144259133907823	23.78353872809748
38	22.473242668489007	25.718464103037054	28.753618292740345	23.05467493573359
39	22.186435867718544	25.70399309192807	28.93016462826996	23.179406412083424
40	21.9808179188267	25.091307760392883	29.510668158980685	23.41720616179973
41	21.497702133027037	25.32096486654488	29.64399584789974	23.537337152528345
42	21.611526354735023	25.0334021174329	29.36658440508809	23.98848712274398
43	21.24378988437446	25.055065437048295	29.699633645835178	24.00151103274207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2782.0
1	2349.0
2	1916.0
3	3804.5
4	5693.0
5	5693.0
6	6244.0
7	6795.0
8	5927.5
9	5060.0
10	7217.5
11	9375.0
12	9375.0
13	14371.0
14	19367.0
15	26072.0
16	32777.0
17	35622.0
18	38467.0
19	38467.0
20	46979.5
21	55492.0
22	61282.5
23	67073.0
24	78338.0
25	89603.0
26	89603.0
27	99528.5
28	109454.0
29	131134.0
30	152814.0
31	165785.0
32	178756.0
33	178756.0
34	192625.5
35	206495.0
36	223551.5
37	240608.0
38	253661.0
39	266714.0
40	266714.0
41	283545.0
42	300376.0
43	292587.0
44	284798.0
45	290932.0
46	297066.0
47	297066.0
48	301050.0
49	305034.0
50	314386.0
51	323738.0
52	331595.5
53	339453.0
54	339453.0
55	308770.5
56	278088.0
57	258428.0
58	238768.0
59	217454.5
60	196141.0
61	196141.0
62	181499.0
63	166857.0
64	148247.0
65	129637.0
66	112642.5
67	95648.0
68	95648.0
69	81113.0
70	66578.0
71	57064.5
72	47551.0
73	39007.5
74	30464.0
75	30464.0
76	24242.5
77	18021.0
78	14638.5
79	11256.0
80	8558.0
81	5860.0
82	5860.0
83	4307.5
84	2755.0
85	2106.5
86	1458.0
87	1037.0
88	616.0
89	616.0
90	480.0
91	344.0
92	229.5
93	115.0
94	78.0
95	41.0
96	41.0
97	34.5
98	28.0
99	21.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4629946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.40816032171824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70059699318372	39.671523106473416
2	11.935067932616183	12.032496355958298
3	3.818400030200464	5.774355626843962
4	1.7751379366388165	3.579257508130143
5	0.9601484032358211	2.419965732147652
6	0.5824449609933274	1.7615987383397151
7	0.41169620268542134	1.4526993732166529
8	0.2850964426613796	1.14969497510611
9	0.2099405578361511	0.9524445567692099
>10	1.1307150042682967	10.368882641079022
>50	0.09112568549123308	3.2044073854041852
>100	0.08242921313890357	8.588685320232422
>500	0.01205773342021934	4.0607683978113185
>1k	0.004970032919673749	4.138955239065735
>5k	8.643535512475761E-5	0.3416796444504969
>10k+	8.643535512475761E-5	0.5025853989717524
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12171	0.2628756361305294	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10899	0.23540231354750143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9159	0.1978208817122273	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6525	0.1409303693822779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.159852404326098E-5	0.0	0.0	0.0
3	0.0	2.159852404326098E-5	0.0	0.0	0.0
4	0.0	2.159852404326098E-5	0.0	0.0	0.0
5	0.0	2.159852404326098E-5	0.0	0.0	0.0
6	0.0	2.159852404326098E-5	0.0	0.0	0.0
7	2.159852404326098E-5	2.159852404326098E-5	0.0	0.0	0.0
8	2.159852404326098E-5	2.159852404326098E-5	0.0	0.0	0.0
9	2.159852404326098E-5	4.319704808652196E-5	0.0	4.319704808652196E-5	0.0
10	2.159852404326098E-5	8.639409617304392E-5	0.0	8.639409617304392E-5	0.0
11	2.159852404326098E-5	1.2959114425956588E-4	0.0	1.943867163893488E-4	0.0
12	2.159852404326098E-5	1.2959114425956588E-4	0.0	4.967660529950025E-4	0.0
13	2.159852404326098E-5	1.2959114425956588E-4	0.0	5.183645770382635E-4	0.0
14	2.159852404326098E-5	1.2959114425956588E-4	0.0	5.831601491680465E-4	0.0
15	2.159852404326098E-5	1.2959114425956588E-4	0.0	6.047586732113074E-4	0.0
16	2.159852404326098E-5	1.2959114425956588E-4	0.0	6.695542453410904E-4	0.0
17	2.159852404326098E-5	1.2959114425956588E-4	0.0	7.775468655573952E-4	0.0
18	2.159852404326098E-5	1.5118966830282685E-4	0.0	8.207439136439172E-4	0.0
19	2.159852404326098E-5	1.5118966830282685E-4	0.0	8.423424376871782E-4	0.0
20	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.001036729154076527	0.0
21	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.0012311158704658759	0.0
22	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.0017062833994176173	0.0
23	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.0025270273130615344	0.0
24	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.004622084145257849	0.0
25	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.0077754686555739526	0.0
26	2.159852404326098E-5	1.5118966830282685E-4	0.0	0.014967777161979858	0.0
27	2.159852404326098E-5	1.7278819234608784E-4	0.0	0.057819248863809646	0.0
28	2.159852404326098E-5	2.159852404326098E-4	0.0	0.14062799004567225	0.0
29	2.159852404326098E-5	2.8078081256239276E-4	0.0	0.23695740727861622	0.0
30	2.159852404326098E-5	2.8078081256239276E-4	0.0	0.3404143374458363	0.0
31	2.159852404326098E-5	2.8078081256239276E-4	0.0	0.5667668694192114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2715	0.0	28.755066	1
CGAATTA	505	0.0	16.48515	15
GTATCAA	4865	0.0	16.00925	2
TGTACCG	200	3.6379788E-12	15.725	5
TAGGACA	1755	0.0	14.336183	4
TTAACGG	235	3.6379788E-12	14.170214	35
CGAGCGT	345	0.0	13.942028	6
CTTAGGA	1825	0.0	13.887672	2
GTATTAG	840	0.0	13.875	1
CCGAATT	650	0.0	13.661538	14
GTACCGA	150	1.3078316E-6	13.566666	6
TTAGGAC	1815	0.0	13.250689	3
GCTTAGG	1780	0.0	12.991572	1
AATCTCG	995	0.0	12.829145	10
GCTACGA	410	0.0	12.634147	2
TAACGGC	280	5.456968E-12	12.553572	36
CGACGTT	135	9.9726494E-5	12.333334	24
GTTTAAG	1050	0.0	12.333334	1
CTTAGAC	360	0.0	12.333334	3
ATAAGCG	105	0.0026456544	12.333333	5
>>END_MODULE