FastQCFastQC Report
Fri 10 Feb 2017
ERR1630029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481060
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.32463235790582423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47970.32388964660445896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36170.2442169797307334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.174537155820831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.025.547621
GTCTAGC705.103564E-621.1428591
TAGACGT701.21949866E-418.54
TTAACGG609.238531E-418.535
GTATCAA18850.016.7824922
TACACCG700.00259296715.8571435
CTAGTAC750.00410514914.83
TCTATAC1702.4378096E-814.1470593
CTAGACT1051.6561395E-414.0952394
AGGGTTG2101.364242E-1014.0952395
TAACGGC800.00629975213.87500136
CCGTATG800.00629975213.8750012
ACAATCG800.00629975213.87500137
GGCTACG1753.56722E-813.7428571
AACGGCC950.0012453713.63157937
GCTACGA1501.306109E-613.5666672
TAGTACC1102.45711E-413.4545454
GTTCGGT850.00940640313.05882425
CGCTATG850.00940640313.0588242
GCTCGCG850.00940640313.05882425
Produced by FastQC (version 0.11.5)