##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630029.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1481060
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7841647198628	34.0	31.0	34.0	31.0	34.0
2	33.02299906823491	34.0	33.0	34.0	31.0	34.0
3	33.155670263189876	34.0	33.0	34.0	31.0	34.0
4	36.45527392543178	37.0	37.0	37.0	35.0	37.0
5	36.40283648197912	37.0	37.0	37.0	35.0	37.0
6	36.4504989669561	37.0	37.0	37.0	35.0	37.0
7	36.3790643187987	37.0	37.0	37.0	35.0	37.0
8	36.38451649494281	37.0	37.0	37.0	35.0	37.0
9	38.146925175212345	39.0	39.0	39.0	37.0	39.0
10	38.11431542273777	39.0	39.0	39.0	37.0	39.0
11	38.15467097889349	39.0	39.0	39.0	37.0	39.0
12	38.0870977543111	39.0	38.0	39.0	37.0	39.0
13	38.10534954694611	39.0	38.0	39.0	37.0	39.0
14	39.54474903109935	41.0	39.0	41.0	37.0	41.0
15	39.52864569970156	41.0	39.0	41.0	37.0	41.0
16	39.442809879410696	41.0	39.0	41.0	37.0	41.0
17	39.37708533077661	41.0	39.0	41.0	36.0	41.0
18	39.268632600974975	41.0	39.0	41.0	36.0	41.0
19	39.293577572819466	41.0	39.0	41.0	36.0	41.0
20	39.50734473957841	41.0	39.0	41.0	37.0	41.0
21	39.51635922920071	41.0	39.0	41.0	37.0	41.0
22	39.52971993032018	41.0	40.0	41.0	37.0	41.0
23	39.46086113999433	41.0	39.0	41.0	37.0	41.0
24	39.4233609711963	41.0	39.0	41.0	36.0	41.0
25	39.39480709761927	41.0	39.0	41.0	36.0	41.0
26	39.24952871591968	41.0	39.0	41.0	36.0	41.0
27	39.16013598368736	41.0	39.0	41.0	36.0	41.0
28	39.11388870133553	41.0	39.0	41.0	36.0	41.0
29	38.99632087828987	40.0	39.0	41.0	35.0	41.0
30	38.90175144828704	40.0	39.0	41.0	35.0	41.0
31	38.7730254007265	40.0	39.0	41.0	35.0	41.0
32	38.68734487461683	40.0	38.0	41.0	35.0	41.0
33	38.60535629886703	40.0	38.0	41.0	35.0	41.0
34	38.528025198168876	40.0	38.0	41.0	35.0	41.0
35	38.388665550349074	40.0	38.0	41.0	35.0	41.0
36	38.37971722955181	40.0	38.0	41.0	35.0	41.0
37	38.32817103966078	40.0	38.0	41.0	34.0	41.0
38	38.230573373124656	40.0	38.0	41.0	34.0	41.0
39	38.172337379984604	40.0	38.0	41.0	34.0	41.0
40	38.111557263041334	40.0	38.0	41.0	34.0	41.0
41	38.00483572576398	40.0	38.0	41.0	34.0	41.0
42	37.90348939273223	40.0	38.0	41.0	33.0	41.0
43	37.6003612277693	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	3.0
14	2.0
15	6.0
16	9.0
17	22.0
18	53.0
19	84.0
20	188.0
21	351.0
22	625.0
23	1075.0
24	1732.0
25	2570.0
26	3513.0
27	5222.0
28	6901.0
29	9298.0
30	12182.0
31	15573.0
32	19557.0
33	25842.0
34	35367.0
35	49231.0
36	74568.0
37	132261.0
38	284373.0
39	800447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.256451460440495	19.180384319338852	12.205312411381037	25.357851808839616
2	18.675678230456565	21.460440495320917	35.141317704887044	24.722563569335477
3	19.054460994152837	23.295747640203636	31.010492485112014	26.63929888053151
4	14.128934681917006	16.039255668237615	35.95094054258436	33.88086910726101
5	14.124478414108815	36.63990655341445	35.15036527892185	14.085249753554887
6	33.68857439941663	36.20454269239598	15.489109151553619	14.617773756633762
7	28.218100549606362	30.954316503045114	22.279043387843842	18.54853955950468
8	26.37644659905743	34.45491742400713	20.336515738727666	18.832120238207768
9	25.835145098780604	14.563218235588025	20.47547027129218	39.126166394339194
10	16.24113810379053	27.170540018635304	34.58988832322796	21.99843355434621
11	34.42169797307334	22.306253629157496	22.494497184448974	20.777551213320187
12	20.38951831796146	25.793958381159438	31.13641581029803	22.68010749058107
13	29.712570726371652	20.752096471446126	25.342187352301732	24.19314544988049
14	21.554089638502155	21.504260462101467	26.790339351545516	30.151310547850862
15	24.001458414919043	28.58135389518318	23.380079132513202	24.037108557384578
16	23.308913886000568	27.637502869566394	25.281285025589778	23.77229821884326
17	22.644929982580045	27.301527284512446	27.014908241394675	23.038634491512834
18	22.831823153687225	26.499736675084062	27.511106909915874	23.157333261312843
19	23.62719943824018	26.088679729383006	27.921421144315556	22.36269968806125
20	23.71949819723711	26.198465963566637	27.478765208701876	22.603270630494375
21	23.076445248673245	26.317299771785073	27.350883826448623	23.255371153093055
22	23.41140804558897	26.293397971722953	27.38707412258788	22.9081198601002
23	23.312627442507395	26.232698202638648	27.23400807529742	23.220666279556536
24	23.128300001350386	26.32047317461818	27.286402981648276	23.26482384238316
25	23.52747356622959	26.047628050180275	27.285660270346913	23.13923811324322
26	23.084885149825123	26.55712800291683	27.32016258625579	23.037824261002253
27	23.022902515765736	26.716473336664283	27.13421468407762	23.126409463492365
28	23.09568822329953	26.524921340121267	27.30348534157968	23.075905094999527
29	22.88799913575412	26.608510121129463	27.590374461534307	22.91311628158211
30	23.030464667197815	26.788178736850632	27.355542651884463	22.825813944067086
31	23.18872969359783	26.397445073123304	27.34345671343497	23.070368519843896
32	22.745736161938073	26.455714150675867	27.733852781116227	23.064696906269834
33	22.666198533482778	26.3066317367291	27.80312748977084	23.224042240017283
34	23.122763426194755	25.84527298016286	27.607996975139425	23.423966618502963
35	22.727641013868446	25.917721091650574	28.098186434040485	23.256451460440495
36	22.779833362591656	25.692477009709265	27.9292533725845	23.59843625511458
37	22.59746397850188	25.065628671356997	28.583717067505702	23.75319028263541
38	22.355205055838386	25.18297705697271	28.959528985996513	23.50228890119239
39	22.005185475267712	25.24158373057135	29.2659986766235	23.48723211753744
40	21.75624215089193	24.949225554670303	29.660243339230014	23.634288955207758
41	21.50770394177143	24.729653086303053	29.876642404764155	23.886000567161357
42	21.08422346157482	25.069139670236183	29.753757443992818	24.092879424196184
43	21.010965119576518	24.856589199627294	30.003375960460748	24.129069720335437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1591.0
1	1215.5
2	840.0
3	1515.5
4	2191.0
5	2191.0
6	2516.5
7	2842.0
8	2437.0
9	2032.0
10	2813.5
11	3595.0
12	3595.0
13	5678.0
14	7761.0
15	9986.0
16	12211.0
17	12559.0
18	12907.0
19	12907.0
20	16398.5
21	19890.0
22	20324.0
23	20758.0
24	24682.0
25	28606.0
26	28606.0
27	32335.5
28	36065.0
29	40037.0
30	44009.0
31	49732.5
32	55456.0
33	55456.0
34	60219.0
35	64982.0
36	70177.5
37	75373.0
38	77751.0
39	80129.0
40	80129.0
41	83247.5
42	86366.0
43	87535.5
44	88705.0
45	90592.5
46	92480.0
47	92480.0
48	92484.0
49	92488.0
50	94377.5
51	96267.0
52	97220.0
53	98173.0
54	98173.0
55	95281.5
56	92390.0
57	87372.0
58	82354.0
59	76961.5
60	71569.0
61	71569.0
62	66433.5
63	61298.0
64	54325.5
65	47353.0
66	41435.0
67	35517.0
68	35517.0
69	29949.5
70	24382.0
71	20518.0
72	16654.0
73	13550.5
74	10447.0
75	10447.0
76	8273.0
77	6099.0
78	4896.0
79	3693.0
80	2753.5
81	1814.0
82	1814.0
83	1383.0
84	952.0
85	703.0
86	454.0
87	325.5
88	197.0
89	197.0
90	149.5
91	102.0
92	69.0
93	36.0
94	25.5
95	15.0
96	15.0
97	11.0
98	7.0
99	8.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1481060.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.49847697682623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.96811631937312	59.901227921056176
2	9.578132195460986	13.504874641254085
3	2.492088753908031	5.270653849247786
4	1.0233006415135697	2.8856454686446353
5	0.5513915059352696	1.9436130693197569
6	0.31996932817360735	1.3534410189322565
7	0.2094132064148071	1.0334318477754343
8	0.1416405251464765	0.7988353040819558
9	0.1092346618094666	0.6930789561581471
>10	0.5513732599522146	7.065816200464435
>50	0.03601164047994354	1.7654792322781672
>100	0.017789488298499155	2.1321238535873954
>500	9.615459587787486E-4	0.41621540741194074
>1k	5.769275752672492E-4	1.2355632297878572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4808	0.32463235790582423	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4797	0.32388964660445896	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.2442169797307334	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2585	0.174537155820831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.751920921502168E-5	0.0
5	0.0	2.02557627645065E-4	0.0	6.751920921502168E-5	0.0
6	0.0	2.02557627645065E-4	0.0	6.751920921502168E-5	0.0
7	0.0	2.02557627645065E-4	0.0	6.751920921502168E-5	0.0
8	0.0	2.02557627645065E-4	0.0	6.751920921502168E-5	0.0
9	0.0	2.02557627645065E-4	0.0	2.02557627645065E-4	0.0
10	0.0	2.02557627645065E-4	0.0	3.3759604607510837E-4	0.0
11	0.0	2.700768368600867E-4	0.0	4.726344645051517E-4	0.0
12	0.0	2.700768368600867E-4	0.0	5.401536737201734E-4	0.0
13	0.0	2.700768368600867E-4	0.0	5.401536737201734E-4	0.0
14	0.0	2.700768368600867E-4	0.0	6.076728829351951E-4	0.0
15	0.0	2.700768368600867E-4	0.0	6.076728829351951E-4	0.0
16	0.0	2.700768368600867E-4	0.0	7.427113013652384E-4	0.0
17	0.0	2.700768368600867E-4	0.0	9.452689290103034E-4	0.0
18	0.0	2.700768368600867E-4	0.0	9.452689290103034E-4	0.0
19	0.0	2.700768368600867E-4	0.0	0.0010127881382253252	0.0
20	0.0	2.700768368600867E-4	0.0	0.0012828649750854118	0.0
21	0.0	2.700768368600867E-4	0.0	0.0015529418119454984	0.0
22	0.0	2.700768368600867E-4	0.0	0.002228133904095715	0.0
23	0.0	3.3759604607510837E-4	0.0	0.003916114134471257	0.0
24	0.0	4.0511525529013E-4	0.0	0.007359593804437362	0.0
25	0.0	4.0511525529013E-4	0.0	0.013031207378499183	0.0
26	0.0	4.0511525529013E-4	0.0	0.023294127179182476	0.0
27	0.0	4.0511525529013E-4	0.0	0.08446653072799211	0.0
28	0.0	4.0511525529013E-4	0.0	0.19560314909591778	0.0
29	0.0	4.0511525529013E-4	0.0	0.3261852997177697	0.0
30	0.0	4.0511525529013E-4	0.0	0.47189175320378646	0.0
31	0.0	4.0511525529013E-4	0.0	0.7635747370126801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1260	0.0	25.54762	1
GTCTAGC	70	5.103564E-6	21.142859	1
TAGACGT	70	1.21949866E-4	18.5	4
TTAACGG	60	9.238531E-4	18.5	35
GTATCAA	1885	0.0	16.782492	2
TACACCG	70	0.002592967	15.857143	5
CTAGTAC	75	0.004105149	14.8	3
TCTATAC	170	2.4378096E-8	14.147059	3
CTAGACT	105	1.6561395E-4	14.095239	4
AGGGTTG	210	1.364242E-10	14.095239	5
TAACGGC	80	0.006299752	13.875001	36
CCGTATG	80	0.006299752	13.875001	2
ACAATCG	80	0.006299752	13.875001	37
GGCTACG	175	3.56722E-8	13.742857	1
AACGGCC	95	0.00124537	13.631579	37
GCTACGA	150	1.306109E-6	13.566667	2
TAGTACC	110	2.45711E-4	13.454545	4
GTTCGGT	85	0.009406403	13.058824	25
CGCTATG	85	0.009406403	13.058824	2
GCTCGCG	85	0.009406403	13.058824	25
>>END_MODULE