FastQCFastQC Report
Fri 10 Feb 2017
ERR1630028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654384
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111900.30620755782643533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102500.2804850283932942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81550.22315662502900624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58130.1590692165902653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.027.8026561
GTACTAG1457.2759576E-1219.137931
GTATCAA42850.017.0968512
CGTGCGA1606.311893E-1016.187510
CGCGCTT3000.016.03333312
TTCGCGG2850.015.57894731
CGGTCGA2800.015.19642920
GTTACAC2700.015.0740753
GTTCGCG2059.276846E-1114.43902430
TATCCCG800.006302085713.8750015
GTCGAAA3400.013.05882322
GTTCTAC9100.013.0109881
TCGCGGA3700.013.032
CGAATTA3500.012.68571415
CGCGGAA3550.012.50704333
TATCAAC59900.012.3848083
GTCTTAG5400.012.3333341
ACGTTAC1050.002645470412.33333326
ATCAACG59800.012.2198994
AAGACCG3051.8189894E-1212.1311475
Produced by FastQC (version 0.11.5)