##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3654384
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82679953721338	34.0	31.0	34.0	31.0	34.0
2	33.058101721110866	34.0	33.0	34.0	31.0	34.0
3	33.18307463036178	34.0	33.0	34.0	31.0	34.0
4	36.483528277269166	37.0	37.0	37.0	35.0	37.0
5	36.428976538864006	37.0	37.0	37.0	35.0	37.0
6	36.47262821859991	37.0	37.0	37.0	35.0	37.0
7	36.40612672340947	37.0	37.0	37.0	35.0	37.0
8	36.40966028747937	37.0	37.0	37.0	35.0	37.0
9	38.17787074374231	39.0	39.0	39.0	37.0	39.0
10	38.15541798563041	39.0	39.0	39.0	37.0	39.0
11	38.1877569516504	39.0	39.0	39.0	37.0	39.0
12	38.126942050972204	39.0	39.0	39.0	37.0	39.0
13	38.141398933445416	39.0	39.0	39.0	37.0	39.0
14	39.597764766921046	41.0	40.0	41.0	37.0	41.0
15	39.58144053826856	41.0	40.0	41.0	37.0	41.0
16	39.50189799429945	41.0	39.0	41.0	37.0	41.0
17	39.43129211380085	41.0	39.0	41.0	37.0	41.0
18	39.32722614810047	41.0	39.0	41.0	36.0	41.0
19	39.35036137417414	41.0	39.0	41.0	36.0	41.0
20	39.564708032872296	41.0	39.0	41.0	37.0	41.0
21	39.56889587957916	41.0	40.0	41.0	37.0	41.0
22	39.586550565019984	41.0	40.0	41.0	37.0	41.0
23	39.51973109558273	41.0	40.0	41.0	37.0	41.0
24	39.48452215202343	41.0	40.0	41.0	37.0	41.0
25	39.45852160035727	41.0	40.0	41.0	37.0	41.0
26	39.317924990915024	41.0	39.0	41.0	36.0	41.0
27	39.235127999684764	41.0	39.0	41.0	36.0	41.0
28	39.19455235136756	41.0	39.0	41.0	36.0	41.0
29	39.08902129606522	41.0	39.0	41.0	36.0	41.0
30	39.00175843589508	40.0	39.0	41.0	35.0	41.0
31	38.87393661968748	40.0	39.0	41.0	35.0	41.0
32	38.791903095022306	40.0	39.0	41.0	35.0	41.0
33	38.71525871391731	40.0	38.0	41.0	35.0	41.0
34	38.64545296827044	40.0	38.0	41.0	35.0	41.0
35	38.515001707538126	40.0	38.0	41.0	35.0	41.0
36	38.501340307969826	40.0	38.0	41.0	35.0	41.0
37	38.46004360789671	40.0	38.0	41.0	35.0	41.0
38	38.36540713838502	40.0	38.0	41.0	35.0	41.0
39	38.31113615865218	40.0	38.0	41.0	34.0	41.0
40	38.25087839701575	40.0	38.0	41.0	34.0	41.0
41	38.149517675208735	40.0	38.0	41.0	34.0	41.0
42	38.05987849114926	40.0	38.0	41.0	34.0	41.0
43	37.76688136769425	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	9.0
15	14.0
16	25.0
17	67.0
18	144.0
19	232.0
20	402.0
21	769.0
22	1413.0
23	2473.0
24	3815.0
25	5787.0
26	8372.0
27	11801.0
28	15808.0
29	21129.0
30	27994.0
31	35940.0
32	46485.0
33	60471.0
34	82620.0
35	115686.0
36	175763.0
37	312083.0
38	682597.0
39	2042478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21527239611382	19.076703488193907	12.167112158984935	25.54091195670734
2	18.652555396477215	21.317382081357625	35.22303622169974	24.807026300465413
3	18.870676973191653	23.312328425255803	31.135507379629505	26.68148722192304
4	14.323918887560804	15.975962022600799	35.79114838506298	33.90897070477541
5	14.06174063809386	36.70380562086524	35.21233674403128	14.02211699700962
6	33.641401669884715	36.02579805515786	15.555480759547985	14.777319515409438
7	28.263285960096145	30.862273915384918	22.29938068905731	18.575059435461625
8	26.139261774351024	34.46178617244384	20.372188582261742	19.026763470943393
9	25.81006812639285	14.660336735274674	20.37916650248031	39.15042863585217
10	16.251521460251578	27.148570046278664	34.582025315347266	22.017883178122496
11	34.238957920131	22.33974316875293	22.649097631775973	20.772201279340102
12	20.29466525685314	25.688351306266664	30.943573527029457	23.073409909850746
13	29.420389318692287	20.695580978900956	25.469709806084968	24.414319896321786
14	21.69640628899426	21.47426214650677	26.562807849421404	30.266523715077557
15	24.162129650304948	28.55728899863835	23.29656653487975	23.984014816176956
16	23.516466797140094	27.49637148148635	25.122592480702632	23.86456924067093
17	22.783210521937487	27.36245014207593	26.710274563373744	23.14406477261284
18	22.67928055727039	26.550083406669906	27.374463110609064	23.396172925450635
19	23.694034343407807	26.06510426928314	27.962660738444562	22.278200648864487
20	23.764552384204833	26.084779267860192	27.433871207842415	22.716797140092556
21	23.01074544984873	26.372816868725344	27.103747170521764	23.512690510904164
22	23.411579078717505	26.230959855340874	27.057282431184028	23.300178634757597
23	23.261868484538024	26.36170692516167	27.06488973244191	23.311534857858398
24	23.312355789648816	26.325503833204174	27.155465873318185	23.206674503828825
25	23.69835791750402	25.78210171673256	27.23189462300623	23.287645742757192
26	23.32784403609473	26.401056922315775	27.187728492681668	23.083370548907833
27	23.13643010696194	26.522445369725787	26.839379769613704	23.501744753698574
28	23.06593943055793	26.439120792998217	27.239337737906034	23.255602038537823
29	23.04656544030403	26.428038213827556	27.531562090902323	22.99383425496609
30	22.97456972228425	26.930010639276002	27.057337159970054	23.038082478469697
31	23.286988997324855	26.297674245508958	27.3226623146336	23.092674442532584
32	22.818756868462646	26.48594126944514	27.46263118490011	23.232670677192107
33	22.78813611268	26.353306056506376	27.61907341976103	23.239484411052587
34	23.242001935209874	25.638219738265057	27.386065613246995	23.73371271327808
35	22.938339265933738	25.831357624157725	27.869293429480862	23.36100968042767
36	22.961872643925762	25.573940779075215	27.815166660099212	23.64901991689981
37	22.854275850594792	24.730104991703115	28.41056112329739	24.005058034404705
38	22.492628032522035	25.14664578216192	28.902956011190945	23.457770174125105
39	22.161108411157667	25.09394196121699	29.231465549323772	23.51348407830157
40	21.900489932092523	24.657479892644012	29.649894482900542	23.792135692362926
41	21.515089820883627	24.679672415378352	29.74824211139278	24.056995652345236
42	21.22390531482187	24.751011387965796	29.687137421792563	24.33794587541977
43	21.103228341630217	24.609893213192702	30.070950398206648	24.215928046970433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3027.0
1	2427.5
2	1828.0
3	3432.0
4	5036.0
5	5036.0
6	5673.0
7	6310.0
8	5460.5
9	4611.0
10	6291.5
11	7972.0
12	7972.0
13	12458.5
14	16945.0
15	22083.0
16	27221.0
17	28431.0
18	29641.0
19	29641.0
20	36779.5
21	43918.0
22	45518.0
23	47118.0
24	54954.5
25	62791.0
26	62791.0
27	71551.5
28	80312.0
29	92359.0
30	104406.0
31	117065.0
32	129724.0
33	129724.0
34	142331.0
35	154938.0
36	168026.5
37	181115.0
38	191498.0
39	201881.0
40	201881.0
41	214483.5
42	227086.0
43	227596.0
44	228106.0
45	233938.0
46	239770.0
47	239770.0
48	240694.5
49	241619.0
50	249841.5
51	258064.0
52	262860.0
53	267656.0
54	267656.0
55	250465.0
56	233274.0
57	215792.5
58	198311.0
59	182224.5
60	166138.0
61	166138.0
62	154469.0
63	142800.0
64	126057.0
65	109314.0
66	95783.0
67	82252.0
68	82252.0
69	68853.0
70	55454.0
71	47421.5
72	39389.0
73	31877.0
74	24365.0
75	24365.0
76	19296.0
77	14227.0
78	11518.0
79	8809.0
80	6687.0
81	4565.0
82	4565.0
83	3454.5
84	2344.0
85	1751.0
86	1158.0
87	820.5
88	483.0
89	483.0
90	362.0
91	241.0
92	167.5
93	94.0
94	71.0
95	48.0
96	48.0
97	30.5
98	13.0
99	11.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3654384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16030443888388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77510380926857	39.78997988647977
2	12.098779718913065	12.379545075171725
3	4.0255327137652515	6.178424374947499
4	1.8788707728229757	3.8449440295577784
5	1.0495979318402568	2.684887486568521
6	0.6488902481348171	1.9918453585200084
7	0.42367415470682956	1.5172709116381728
8	0.30786183685247964	1.2600244238789486
9	0.23383576457455105	1.0766798013899888
>10	1.3388466089242788	12.7261072280027
>50	0.12106306183041352	4.317205090079197
>100	0.09141580340664773	8.656121491406275
>500	0.005286796252029966	1.8466205565417628
>1k	0.0010249911064836143	0.7531047855029979
>5k	1.0789380068248572E-4	0.3855083975484983
>10k+	1.0789380068248572E-4	0.5917311027663089
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11190	0.30620755782643533	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10250	0.2804850283932942	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8155	0.22315662502900624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5813	0.1590692165902653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.4728786027959845E-5	0.0	0.0	0.0
3	0.0	5.4728786027959845E-5	0.0	0.0	0.0
4	0.0	8.209317904193976E-5	0.0	0.0	0.0
5	0.0	1.0945757205591969E-4	0.0	0.0	0.0
6	0.0	1.0945757205591969E-4	0.0	0.0	0.0
7	0.0	1.0945757205591969E-4	0.0	0.0	0.0
8	0.0	1.0945757205591969E-4	0.0	2.7364393013979922E-5	0.0
9	0.0	1.0945757205591969E-4	0.0	8.209317904193976E-5	2.7364393013979922E-5
10	0.0	1.0945757205591969E-4	0.0	2.4627953712581926E-4	2.7364393013979922E-5
11	0.0	1.3682196506989961E-4	0.0	3.5573710918173897E-4	2.7364393013979922E-5
12	0.0	1.3682196506989961E-4	0.0	9.030249694613374E-4	2.7364393013979922E-5
13	0.0	1.3682196506989961E-4	0.0	9.303893624753173E-4	2.7364393013979922E-5
14	0.0	1.3682196506989961E-4	0.0	9.303893624753173E-4	2.7364393013979922E-5
15	0.0	1.3682196506989961E-4	0.0	9.85118148503277E-4	2.7364393013979922E-5
16	0.0	1.3682196506989961E-4	0.0	0.0010124825415172571	2.7364393013979922E-5
17	0.0	1.3682196506989961E-4	0.0	0.001039846934531237	2.7364393013979922E-5
18	0.0	1.3682196506989961E-4	0.0	0.0011766688996011367	2.7364393013979922E-5
19	0.0	1.3682196506989961E-4	0.0	0.0013682196506989961	2.7364393013979922E-5
20	0.0	1.3682196506989961E-4	0.0	0.0015324060087828755	2.7364393013979922E-5
21	0.0	1.3682196506989961E-4	0.0	0.0019155075109785944	2.7364393013979922E-5
22	0.0	1.3682196506989961E-4	0.0	0.003174269589621671	2.7364393013979922E-5
23	0.0	2.1891514411183938E-4	0.0	0.004487760454292707	2.7364393013979922E-5
24	0.0	2.1891514411183938E-4	0.0	0.008017767153096118	2.7364393013979922E-5
25	0.0	2.1891514411183938E-4	0.0	0.012970722288626483	2.7364393013979922E-5
26	0.0	2.1891514411183938E-4	0.0	0.022219887127351696	2.7364393013979922E-5
27	0.0	2.1891514411183938E-4	0.0	0.08261310250920538	2.7364393013979922E-5
28	0.0	2.7364393013979923E-4	0.0	0.18865012543837759	2.7364393013979922E-5
29	0.0	2.7364393013979923E-4	0.0	0.30713794718891063	2.7364393013979922E-5
30	0.0	2.7364393013979923E-4	0.0	0.44335789561250266	2.7364393013979922E-5
31	0.0	2.7364393013979923E-4	0.0	0.7156062417085889	2.7364393013979922E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2635	0.0	27.802656	1
GTACTAG	145	7.2759576E-12	19.13793	1
GTATCAA	4285	0.0	17.096851	2
CGTGCGA	160	6.311893E-10	16.1875	10
CGCGCTT	300	0.0	16.033333	12
TTCGCGG	285	0.0	15.578947	31
CGGTCGA	280	0.0	15.196429	20
GTTACAC	270	0.0	15.074075	3
GTTCGCG	205	9.276846E-11	14.439024	30
TATCCCG	80	0.0063020857	13.875001	5
GTCGAAA	340	0.0	13.058823	22
GTTCTAC	910	0.0	13.010988	1
TCGCGGA	370	0.0	13.0	32
CGAATTA	350	0.0	12.685714	15
CGCGGAA	355	0.0	12.507043	33
TATCAAC	5990	0.0	12.384808	3
GTCTTAG	540	0.0	12.333334	1
ACGTTAC	105	0.0026454704	12.333333	26
ATCAACG	5980	0.0	12.219899	4
AAGACCG	305	1.8189894E-12	12.131147	5
>>END_MODULE