FastQCFastQC Report
Fri 10 Feb 2017
ERR1630027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5128201
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118870.2317966865963327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT107330.2092936684814031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89690.17489564079099085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65320.12737410253615256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.027.3288361
CGTGCGA2500.017.7610
GTATCAA49450.016.2740142
GTATTAG11750.014.48510551
TTAGTAC3450.013.9420293
GTATAGG7700.013.6948051
TATACTG7600.013.3881585
ATCTGCG6950.013.30935336
CGACGTT1703.7386963E-713.05882324
AATCTCG8500.012.84117510
GTACTAG2451.1459633E-1012.8367341
TCTATAC5500.012.7818183
TAATACG3050.012.7377054
ATACCGT1901.048229E-712.6578956
AATACGC3950.012.645575
ATCAACG63500.012.6440944
TCAACGC64200.012.5926785
CGAATTA5450.012.55963315
TTAGGCA6500.012.5230774
CTAGCAC9100.012.4010983
Produced by FastQC (version 0.11.5)