##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5128201
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4285257149632	33.0	31.0	34.0	31.0	34.0
2	32.70789112985236	34.0	31.0	34.0	31.0	34.0
3	32.8608870830141	34.0	31.0	34.0	31.0	34.0
4	36.31848615138135	37.0	37.0	37.0	35.0	37.0
5	36.21892024123079	37.0	35.0	37.0	35.0	37.0
6	36.285346069703586	37.0	37.0	37.0	35.0	37.0
7	36.21886232618417	37.0	37.0	37.0	35.0	37.0
8	36.21623489406909	37.0	37.0	37.0	35.0	37.0
9	37.90798586092862	39.0	38.0	39.0	35.0	39.0
10	37.82315786764208	39.0	38.0	39.0	35.0	39.0
11	37.8987065834588	39.0	38.0	39.0	35.0	39.0
12	37.80093779475492	39.0	38.0	39.0	35.0	39.0
13	37.836474233361756	39.0	38.0	39.0	35.0	39.0
14	39.184258378327996	40.0	39.0	41.0	36.0	41.0
15	39.16876542085616	40.0	39.0	41.0	36.0	41.0
16	39.081805295853265	40.0	38.0	41.0	36.0	41.0
17	39.002431456957325	40.0	38.0	41.0	36.0	41.0
18	38.86510318140806	40.0	38.0	41.0	36.0	41.0
19	38.899602999180416	40.0	38.0	41.0	35.0	41.0
20	39.099839495370794	40.0	39.0	41.0	36.0	41.0
21	39.09889920461386	40.0	39.0	41.0	36.0	41.0
22	39.11679300401837	40.0	39.0	41.0	36.0	41.0
23	39.05013220035642	40.0	39.0	41.0	36.0	41.0
24	39.00311064250407	40.0	39.0	41.0	36.0	41.0
25	38.97347412864667	40.0	39.0	41.0	36.0	41.0
26	38.81421457544273	40.0	38.0	41.0	35.0	41.0
27	38.70784861981814	40.0	38.0	41.0	35.0	41.0
28	38.664092924594804	40.0	38.0	41.0	35.0	41.0
29	38.543997397917906	40.0	38.0	41.0	35.0	41.0
30	38.454348415750474	40.0	38.0	41.0	34.0	41.0
31	38.329978875632996	40.0	38.0	41.0	34.0	41.0
32	38.25003446627775	40.0	38.0	41.0	34.0	41.0
33	38.169218406220814	40.0	38.0	41.0	34.0	41.0
34	38.09925897990348	40.0	38.0	41.0	34.0	41.0
35	37.95261749685709	40.0	38.0	41.0	33.0	41.0
36	37.947969278115266	40.0	38.0	41.0	33.0	41.0
37	37.901575425768215	40.0	38.0	41.0	33.0	41.0
38	37.80276377622484	40.0	38.0	41.0	33.0	41.0
39	37.75871285076384	40.0	38.0	41.0	33.0	41.0
40	37.687423133375624	40.0	37.0	41.0	33.0	41.0
41	37.57303818629574	40.0	37.0	41.0	33.0	41.0
42	37.47979457123463	40.0	37.0	41.0	33.0	41.0
43	37.166452328994126	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	7.0
15	22.0
16	47.0
17	106.0
18	216.0
19	382.0
20	737.0
21	1468.0
22	2587.0
23	4310.0
24	6903.0
25	10393.0
26	14574.0
27	20227.0
28	27467.0
29	37433.0
30	49121.0
31	63642.0
32	81555.0
33	108137.0
34	147635.0
35	210423.0
36	327592.0
37	605519.0
38	1451298.0
39	1956395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91912504989567	19.407995123436077	12.010332668317798	25.662547158350463
2	18.364432283367986	21.307589152609268	35.91105340839799	24.41692515562475
3	18.783780120942996	23.888533230269253	31.18315760244187	26.144529046345884
4	14.034668297907979	15.97569986043839	36.621906980635124	33.36772486101851
5	13.637842978463596	37.303569029373065	35.57064163436652	13.487946357796817
6	33.310453314914916	36.87864418730857	15.638505588996999	14.172396908779511
7	28.01812955459429	31.11128054458084	22.772196331618048	18.098393569206824
8	25.93999338169467	35.377727979071025	20.327342863511006	18.3549357757233
9	25.906979855118784	14.652916295597617	20.165863233519904	39.274240615763695
10	15.849105758530136	27.301211477475242	34.98932666640797	21.86035609758666
11	34.28668260077949	22.57298417125226	22.844794890059887	20.29553833790836
12	20.250454301615715	25.800295269237694	31.234930144118767	22.714320285027828
13	29.08534591370346	20.73393769081984	25.9617553992131	24.218960996263604
14	21.520080823665065	21.337326676547974	26.673720472344982	30.468872027441986
15	23.93665926901071	28.801952185571512	23.443055371659575	23.818333173758205
16	23.486325906492354	27.701644299823663	24.951927586301707	23.860102207382276
17	22.680078257462995	27.605138722136672	26.68859898432218	23.02618403607815
18	22.488022602858194	26.682222479189093	27.532657163789015	23.297097754163694
19	23.636144527096345	26.219428606640026	27.919478975180574	22.22494789108305
20	23.573763976880002	26.24795712960549	27.65186075974791	22.526418133766597
21	23.03965854692513	26.612919423400133	27.01385924615669	23.33356278351804
22	23.222471194089312	26.462457302278132	27.214338907542825	23.100732596089742
23	23.143184130263226	26.64881895229926	27.14425585112596	23.063741066311557
24	23.43069236170735	26.572632390969076	27.069239290737627	22.927435956585946
25	23.64332053287303	25.96926290525664	27.379484540485056	23.007932021385276
26	23.38028482112928	26.579398896416112	27.14187684921086	22.898439433243745
27	23.222685694261987	26.70538849783774	26.709600501228405	23.36232530667187
28	23.027178536878722	26.420161768230223	27.40836406373307	23.14429563115798
29	23.14371063068706	26.622747431311684	27.430906081879396	22.802635856121864
30	22.945395471043355	27.010076243111374	27.108707322509396	22.935820963335875
31	23.236160205108966	26.458284298918862	27.38863004784719	22.916925448124985
32	22.68961376513908	26.64581594988184	27.558923684933568	23.105646600045514
33	22.864314405773097	26.5559793775634	27.51522414975544	23.064482066908067
34	23.211161184984753	26.044084465487995	27.439661588927578	23.30509276059967
35	23.16785165012058	26.220735107691763	27.657047763923448	22.95436547826421
36	23.202503178015057	26.148117049234227	27.284694964179444	23.36468480857127
37	23.044475050802415	25.18175477131259	27.938608490579835	23.83516168730516
38	22.899745934295478	25.64117514114599	28.317571795645296	23.14150712891324
39	22.39955103163858	25.713637199477944	28.539949974659727	23.346861794223745
40	22.37973901568991	24.986540114164793	29.173193484420757	23.460527385724543
41	21.866888602845325	25.17354526470394	29.260300054541545	23.699266077909193
42	21.791540542190134	25.194819781829924	28.9604093131295	24.053230362850442
43	21.684641456136372	24.863066014768144	29.464328718784614	23.987963810310866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2384.0
1	2044.5
2	1705.0
3	3347.5
4	4990.0
5	4990.0
6	5811.5
7	6633.0
8	5711.5
9	4790.0
10	6945.5
11	9101.0
12	9101.0
13	13964.0
14	18827.0
15	25109.5
16	31392.0
17	34134.5
18	36877.0
19	36877.0
20	46782.5
21	56688.0
22	62377.0
23	68066.0
24	79853.5
25	91641.0
26	91641.0
27	103958.5
28	116276.0
29	137978.5
30	159681.0
31	176506.5
32	193332.0
33	193332.0
34	212802.5
35	232273.0
36	252376.5
37	272480.0
38	287149.0
39	301818.0
40	301818.0
41	321348.0
42	340878.0
43	339051.5
44	337225.0
45	340065.5
46	342906.0
47	342906.0
48	343184.0
49	343462.0
50	351511.5
51	359561.0
52	361022.0
53	362483.0
54	362483.0
55	337309.5
56	312136.0
57	286435.5
58	260735.0
59	237425.5
60	214116.0
61	214116.0
62	199033.5
63	183951.0
64	161441.5
65	138932.0
66	120741.0
67	102550.0
68	102550.0
69	87136.5
70	71723.0
71	62099.0
72	52475.0
73	46208.5
74	39942.0
75	39942.0
76	33838.5
77	27735.0
78	21978.5
79	16222.0
80	11470.5
81	6719.0
82	6719.0
83	4828.0
84	2937.0
85	2173.5
86	1410.0
87	1037.5
88	665.0
89	665.0
90	484.0
91	303.0
92	208.5
93	114.0
94	78.5
95	43.0
96	43.0
97	28.5
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5128201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.704789183726675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57817842225948	38.271336144974086
2	11.437156490430155	11.14088591455384
3	3.8395124386556736	5.610079316790626
4	1.8333449185121566	3.571707110287646
5	1.040461528417624	2.533772969767921
6	0.6505613950026079	1.901127335680389
7	0.43860175039176946	1.4953404051911252
8	0.32157753779195775	1.252989294750336
9	0.24599561914634485	1.0783048293578739
>10	1.3718411607148393	12.179279412493461
>50	0.12428767376969853	4.247454511002145
>100	0.10576278826907495	10.531665109367932
>500	0.009619204487132847	3.1833778280666554
>1k	0.002938081390342947	2.255410906181087
>5k	8.049538055727175E-5	0.30385916942639885
>10k+	8.049538055727175E-5	0.4434097421085828
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11887	0.2317966865963327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10733	0.2092936684814031	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8969	0.17489564079099085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6532	0.12737410253615256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9500015697512636E-5	0.0
3	0.0	0.0	0.0	1.9500015697512636E-5	0.0
4	0.0	0.0	0.0	3.900003139502527E-5	0.0
5	1.1700009418507582E-4	0.0	0.0	3.900003139502527E-5	0.0
6	1.1700009418507582E-4	0.0	0.0	3.900003139502527E-5	0.0
7	1.3650010988258845E-4	0.0	0.0	3.900003139502527E-5	0.0
8	1.3650010988258845E-4	0.0	0.0	3.900003139502527E-5	0.0
9	1.3650010988258845E-4	0.0	0.0	2.3400018837015164E-4	0.0
10	1.9500015697512636E-4	0.0	0.0	3.5100028255522745E-4	0.0
11	2.535002040676643E-4	0.0	0.0	4.29000345345278E-4	0.0
12	2.730002197651769E-4	0.0	0.0	6.045004866228917E-4	0.0
13	3.1200025116020217E-4	1.9500015697512636E-5	0.0	6.045004866228917E-4	0.0
14	3.1200025116020217E-4	1.9500015697512636E-5	0.0	6.045004866228917E-4	0.0
15	4.485003610427906E-4	1.9500015697512636E-5	0.0	6.240005023204043E-4	0.0
16	4.485003610427906E-4	1.9500015697512636E-5	0.0	6.435005180179171E-4	0.0
17	5.460004395303538E-4	1.9500015697512636E-5	0.0	7.020005651104549E-4	0.0
18	5.460004395303538E-4	5.850004709253791E-5	0.0	7.605006122029928E-4	0.0
19	5.655004552278665E-4	5.850004709253791E-5	0.0	8.385006749930434E-4	0.0
20	5.655004552278665E-4	5.850004709253791E-5	0.0	0.0010530008476656823	0.0
21	6.045004866228917E-4	5.850004709253791E-5	0.0	0.0012480010046408087	0.0
22	6.240005023204043E-4	5.850004709253791E-5	0.0	0.0015990012871960361	0.0
23	6.240005023204043E-4	7.800006279005054E-5	0.0	0.0023790019150965415	0.0
24	6.240005023204043E-4	7.800006279005054E-5	0.0	0.004212003390662729	0.0
25	6.240005023204043E-4	7.800006279005054E-5	0.0	0.006649505352851809	0.0
26	6.240005023204043E-4	7.800006279005054E-5	0.0	0.012168009795247885	0.0
27	6.630005337154297E-4	7.800006279005054E-5	0.0	0.05132404131585326	0.0
28	7.995006435980181E-4	7.800006279005054E-5	0.0	0.1280956031169605	0.0
29	7.995006435980181E-4	1.1700009418507582E-4	0.0	0.21875117609469677	0.0
30	7.995006435980181E-4	1.1700009418507582E-4	0.0	0.3212627586165207	0.0
31	7.995006435980181E-4	1.1700009418507582E-4	0.0	0.5253109228752929	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2965	0.0	27.328836	1
CGTGCGA	250	0.0	17.76	10
GTATCAA	4945	0.0	16.274014	2
GTATTAG	1175	0.0	14.4851055	1
TTAGTAC	345	0.0	13.942029	3
GTATAGG	770	0.0	13.694805	1
TATACTG	760	0.0	13.388158	5
ATCTGCG	695	0.0	13.309353	36
CGACGTT	170	3.7386963E-7	13.058823	24
AATCTCG	850	0.0	12.841175	10
GTACTAG	245	1.1459633E-10	12.836734	1
TCTATAC	550	0.0	12.781818	3
TAATACG	305	0.0	12.737705	4
ATACCGT	190	1.048229E-7	12.657895	6
AATACGC	395	0.0	12.64557	5
ATCAACG	6350	0.0	12.644094	4
TCAACGC	6420	0.0	12.592678	5
CGAATTA	545	0.0	12.559633	15
TTAGGCA	650	0.0	12.523077	4
CTAGCAC	910	0.0	12.401098	3
>>END_MODULE