FastQCFastQC Report
Fri 10 Feb 2017
ERR1630026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997847
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51660.5177146396190999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47920.4802339436807446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35220.3529599227136024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.26016012474858374No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG17050.17086787854250202No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.11635050263216705No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11200.11224165628598373No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC10420.10442482665178128No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG10210.10232029559641909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.027.3540841
CGTCAGA759.263762E-619.7333341
AGACCGT851.2449709E-619.5882346
GGGCCGT500.007034246318.56
GTATCAA19750.017.7974682
CGAATTA752.0669219E-417.26666615
GGATTGT904.4450113E-516.4444456
ATTAAGC1059.340549E-615.8571423
AATGCGT700.002592172715.85714235
GGTATAT700.002592172715.8571421
TGCGTTA700.002592172715.85714237
GGTTTAG957.0581824E-515.5789481
GTCTTAC957.0581824E-515.5789481
GACCGTG1101.4514075E-515.1363647
GGGTAAG1101.4514075E-515.1363641
GTATAGG1101.4514075E-515.1363641
GGGTAGA1353.9703082E-715.0740751
GGAGCGT750.004103901314.86
AAATGCG750.004103901314.834
CGACCAA908.272666E-414.38888812
Produced by FastQC (version 0.11.5)