FastQCFastQC Report
Fri 10 Feb 2017
ERR1630025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504844
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112640.4496886832074173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108450.4329610945831357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82350.3287629888328375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.23973548851744858No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG56200.22436526985313257No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG49070.19590042333973695No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG46640.1861992203905712No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT42640.1702301620380351No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC40110.160129732630056No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC39920.15937120235831054No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA37730.15062814291029702No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC33350.13314202401426997No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC31250.12475826837918848No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT28950.11557605982648021No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.11246209344773567No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA28010.11182333111363424No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT27470.10966750823604185No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG27080.10811052504666957No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG26550.10599462481495853No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.10463725485499298No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA25950.1035992660620781No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG25610.10224189610211255No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC25580.10212212816446853No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC25540.10196243758094316No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG25250.1008046808503843No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA25110.10024576380804552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.023.6439021
TTAACGG2200.018.535
CGTGCGA2500.017.7610
TAACGGC2300.017.69565236
GTATACG953.6098245E-617.5263161
TACACGC650.001580637617.0769235
CGTCGCG803.3845584E-416.187515
GTATCAA48900.014.90593052
CGACCAA2500.014.79999912
GAGTAGA3900.014.7051291
GACGGTT1406.0025013E-714.53571411
GTATTAG7150.014.4895111
TTGTACG908.278817E-414.3888899
CCGTGCG3150.014.0952389
TATTAGA5300.013.9622642
AGTGTCG800.00630135713.8755
TCGTTGT2551.8189894E-1213.78431334
GTGCGAG3100.013.72580711
TAATACG1501.307144E-613.5666674
CTAGACT2051.4260877E-913.5365864
Produced by FastQC (version 0.11.5)