##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630025.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504844
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82143239259611	34.0	31.0	34.0	31.0	34.0
2	33.04038215553543	34.0	33.0	34.0	31.0	34.0
3	33.168971001786936	34.0	33.0	34.0	31.0	34.0
4	36.46246353066299	37.0	37.0	37.0	35.0	37.0
5	36.42221870902939	37.0	37.0	37.0	35.0	37.0
6	36.46139440220629	37.0	37.0	37.0	35.0	37.0
7	36.384527339826356	37.0	37.0	37.0	35.0	37.0
8	36.393509136696736	37.0	37.0	37.0	35.0	37.0
9	38.16768748872185	39.0	39.0	39.0	37.0	39.0
10	38.14946160319764	39.0	39.0	39.0	37.0	39.0
11	38.18198099362675	39.0	39.0	39.0	37.0	39.0
12	38.094084901095634	39.0	39.0	39.0	37.0	39.0
13	38.11872356122777	39.0	39.0	39.0	37.0	39.0
14	39.57433796276335	41.0	40.0	41.0	37.0	41.0
15	39.552826443483106	41.0	39.0	41.0	37.0	41.0
16	39.48509527938666	41.0	39.0	41.0	37.0	41.0
17	39.39709898101439	41.0	39.0	41.0	36.0	41.0
18	39.28786742807137	41.0	39.0	41.0	36.0	41.0
19	39.32851147616378	41.0	39.0	41.0	36.0	41.0
20	39.544740910012756	41.0	39.0	41.0	37.0	41.0
21	39.55603143349446	41.0	40.0	41.0	37.0	41.0
22	39.5735047771438	41.0	40.0	41.0	37.0	41.0
23	39.51703818680924	41.0	40.0	41.0	37.0	41.0
24	39.47869328389313	41.0	40.0	41.0	37.0	41.0
25	39.444681185734524	41.0	39.0	41.0	36.0	41.0
26	39.30103990507992	41.0	39.0	41.0	36.0	41.0
27	39.20189161480715	41.0	39.0	41.0	36.0	41.0
28	39.161420830997855	41.0	39.0	41.0	36.0	41.0
29	39.053008490748326	41.0	39.0	41.0	35.0	41.0
30	38.96502297149044	40.0	39.0	41.0	35.0	41.0
31	38.80847988936636	40.0	39.0	41.0	35.0	41.0
32	38.702048111579	40.0	38.0	41.0	35.0	41.0
33	38.63201061622999	40.0	38.0	41.0	35.0	41.0
34	38.51861393364217	40.0	38.0	41.0	35.0	41.0
35	38.41348842482805	40.0	38.0	41.0	35.0	41.0
36	38.37537986397556	40.0	38.0	41.0	35.0	41.0
37	38.29955518187959	40.0	38.0	41.0	34.0	41.0
38	38.21510840595263	40.0	38.0	41.0	34.0	41.0
39	38.11907767509673	40.0	38.0	41.0	34.0	41.0
40	38.04923140922149	40.0	38.0	41.0	34.0	41.0
41	37.926264469963	40.0	38.0	41.0	33.0	41.0
42	37.816194541456476	40.0	38.0	41.0	33.0	41.0
43	37.511908526039946	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	8.0
16	18.0
17	31.0
18	60.0
19	119.0
20	250.0
21	532.0
22	951.0
23	1618.0
24	2731.0
25	4099.0
26	6082.0
27	8587.0
28	11945.0
29	15912.0
30	20866.0
31	26415.0
32	33390.0
33	42893.0
34	59060.0
35	82678.0
36	126721.0
37	232603.0
38	457575.0
39	1369695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51055634602395	19.121071012805587	11.868683239355425	24.499689401815044
2	18.85634394796642	21.601904150517957	35.03423766110784	24.507514240407787
3	19.569801552511855	22.767405874377804	30.20327812829861	27.459514444811735
4	13.368097973366805	16.10024416690221	35.79592182187793	34.73573603785305
5	14.450680361731111	36.298667701461646	34.68435559260377	14.566296344203472
6	34.276266306404715	35.61088035821792	15.482481144534349	14.630372190843023
7	29.22309732661994	30.191461025117732	21.603141752540278	18.98229989572205
8	26.291337903677835	33.946904477883656	20.45073465652951	19.311022961909003
9	25.26931816911552	13.993047071993306	20.54487225551771	40.192762503373466
10	16.242967625928	26.942396412710735	34.60674596901045	22.20788999235082
11	34.529575494521815	22.580927195466067	21.638153912978215	21.251343397033907
12	20.48171462973343	25.465617818914073	30.746824951973057	23.305842599379442
13	30.0242250615208	20.58986507742598	25.259537120874594	24.12637274017863
14	21.9135403242677	21.581862982285525	26.344674558575303	30.159922134871476
15	23.46509403380011	28.756720977434124	24.246579826927345	23.531605161838424
16	24.30390874641295	26.645611463228846	25.07229991169111	23.978179878667095
17	22.79259706392893	26.833128130933503	26.436855947915323	23.937418857222244
18	22.32286721248908	25.63656658857797	28.00513724607201	24.035428952860936
19	24.87224753317971	25.747711234711623	27.67062539623226	21.709415835876406
20	24.312651805860963	25.975988923861127	27.431927896507723	22.279431373770183
21	22.683488472735228	26.237921403488603	27.347012428718116	23.731577695058057
22	23.90683811047714	25.41008541849313	27.39444053202515	23.288635939004585
23	21.99969339407963	26.03906670435364	27.784684395515253	24.176555506051475
24	22.940949616023993	26.544487401211413	27.206285101986396	23.308277880778206
25	23.342052439193818	26.10238402072145	27.38609669903595	23.169466841048784
26	23.888513616017605	26.19280881364269	26.538658694912737	23.380018875426973
27	23.44844629046759	26.058628800835503	26.489034846082234	24.003890062614676
28	22.990533542208617	26.185183588279347	27.569221875693657	23.255060993818375
29	23.319935293375554	26.612036518042643	27.816382976345032	22.25164521223677
30	23.393233271213695	25.898938217310143	26.814085028848105	23.89374348262806
31	22.580368278423727	25.41615366066709	27.936789676323155	24.066688384586026
32	22.174075511289328	26.29592900795419	27.61493330522779	23.915062175528696
33	22.750079446065303	25.632853782511006	27.68523708462483	23.93182968679886
34	23.570090592468034	25.44669448476632	28.099674071519026	22.883540851246625
35	23.36987852337311	25.650140288177624	27.06623646023465	23.91374472821461
36	22.68057411958589	25.346808024771207	27.02559520672744	24.947022648915464
37	22.1495230840723	24.91831826652678	29.117462005617917	23.814696643783005
38	22.142895924856	24.49929017535623	28.500058287062984	24.857755612724787
39	21.564895857785952	25.29930007617241	29.71881682052854	23.416987245513095
40	22.502519118955114	24.46715244542175	29.229405104669194	23.800923330953943
41	21.31154674702297	24.862426562292903	29.555613044165625	24.270413646518506
42	20.616174101061784	25.666428727697216	29.333962514232425	24.38343465700858
43	21.449878715001812	24.202505225874347	29.185929343304412	25.161686715819425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2927.0
1	2361.0
2	1795.0
3	3324.5
4	4854.0
5	4854.0
6	5729.5
7	6605.0
8	5852.0
9	5099.0
10	6324.0
11	7549.0
12	7549.0
13	12128.5
14	16708.0
15	21091.0
16	25474.0
17	25147.0
18	24820.0
19	24820.0
20	29072.5
21	33325.0
22	32179.5
23	31034.0
24	35644.0
25	40254.0
26	40254.0
27	45212.0
28	50170.0
29	57263.5
30	64357.0
31	72447.5
32	80538.0
33	80538.0
34	97250.5
35	113963.0
36	121553.5
37	129144.0
38	129394.5
39	129645.0
40	129645.0
41	133203.5
42	136762.0
43	145624.5
44	154487.0
45	151063.0
46	147639.0
47	147639.0
48	146173.5
49	144708.0
50	150680.0
51	156652.0
52	164882.5
53	173113.0
54	173113.0
55	174111.5
56	175110.0
57	155641.0
58	136172.0
59	132350.0
60	128528.0
61	128528.0
62	121500.5
63	114473.0
64	92825.5
65	71178.0
66	63561.0
67	55944.0
68	55944.0
69	49687.5
70	43431.0
71	44908.0
72	46385.0
73	41032.0
74	35679.0
75	35679.0
76	21615.5
77	7552.0
78	5861.5
79	4171.0
80	3227.5
81	2284.0
82	2284.0
83	1752.0
84	1220.0
85	890.0
86	560.0
87	422.5
88	285.0
89	285.0
90	224.0
91	163.0
92	103.0
93	43.0
94	35.0
95	27.0
96	27.0
97	18.5
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2504844.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.63451768124943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41930251776989	42.85465717966556
2	11.269806859705579	11.863616968428788
3	3.2444694827852247	5.123132590737993
4	1.3140088222862203	2.766488823597672
5	0.7046918140219344	1.854555687248462
6	0.4004801284886063	1.2647447042353555
7	0.27184033007167224	1.001572926174388
8	0.18943596795052908	0.7976696643645378
9	0.15168309201734387	0.7185389749860123
>10	0.8649663692660915	8.761149314231393
>50	0.09072508848213824	3.322850121516506
>100	0.059753056872035804	5.966956567213632
>500	0.00991393172779605	3.7274450696900843
>1k	0.008541233488562418	8.29200184199658
>5k	2.2878303987220765E-4	0.7971727841748294
>10k+	1.5252202658147177E-4	0.8874467817382328
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11264	0.4496886832074173	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10845	0.4329610945831357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8235	0.3287629888328375	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6005	0.23973548851744858	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5620	0.22436526985313257	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	4907	0.19590042333973695	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	4664	0.1861992203905712	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	4264	0.1702301620380351	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	4011	0.160129732630056	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	3992	0.15937120235831054	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	3773	0.15062814291029702	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3335	0.13314202401426997	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	3125	0.12475826837918848	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2895	0.11557605982648021	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2817	0.11246209344773567	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2801	0.11182333111363424	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2747	0.10966750823604185	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2708	0.10811052504666957	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2655	0.10599462481495853	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2621	0.10463725485499298	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2595	0.1035992660620781	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2561	0.10224189610211255	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	2558	0.10212212816446853	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2554	0.10196243758094316	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	2525	0.1008046808503843	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2511	0.10024576380804552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.9922645881340315E-5	0.0
8	0.0	0.0	0.0	3.9922645881340315E-5	0.0
9	0.0	0.0	0.0	1.1976793764402095E-4	0.0
10	0.0	0.0	0.0	1.1976793764402095E-4	0.0
11	0.0	0.0	0.0	1.9961322940670158E-4	0.0
12	0.0	0.0	0.0	2.395358752880419E-4	0.0
13	0.0	0.0	0.0	2.395358752880419E-4	0.0
14	0.0	0.0	0.0	2.794585211693822E-4	0.0
15	3.9922645881340315E-5	0.0	0.0	3.193811670507225E-4	0.0
16	3.9922645881340315E-5	0.0	0.0	3.9922645881340315E-4	0.0
17	7.984529176268063E-5	0.0	0.0	5.189943964574241E-4	0.0
18	7.984529176268063E-5	3.9922645881340315E-5	0.0	7.186076258641256E-4	0.0
19	7.984529176268063E-5	3.9922645881340315E-5	0.0	9.182208552708272E-4	0.0
20	7.984529176268063E-5	3.9922645881340315E-5	0.0	0.00127752466820289	0.0
21	7.984529176268063E-5	3.9922645881340315E-5	0.0	0.001636828481134953	0.0
22	7.984529176268063E-5	3.9922645881340315E-5	0.0	0.0031938116705072252	0.0
23	7.984529176268063E-5	7.984529176268063E-5	0.0	0.00503025338104888	0.0
24	7.984529176268063E-5	7.984529176268063E-5	0.0	0.009182208552708272	0.0
25	7.984529176268063E-5	7.984529176268063E-5	0.0	0.014931069559621278	0.0
26	7.984529176268063E-5	7.984529176268063E-5	0.0	0.02810554270046358	0.0
27	1.1976793764402095E-4	7.984529176268063E-5	0.0	0.08898757766950756	0.0
28	1.5969058352536126E-4	1.1976793764402095E-4	0.0	0.21178963640051038	0.0
29	1.5969058352536126E-4	1.5969058352536126E-4	0.0	0.3506006761299306	0.0
30	1.5969058352536126E-4	1.5969058352536126E-4	0.0	0.4906493178816725	0.0
31	1.5969058352536126E-4	1.5969058352536126E-4	0.0	0.7968560117915527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3075	0.0	23.643902	1
TTAACGG	220	0.0	18.5	35
CGTGCGA	250	0.0	17.76	10
TAACGGC	230	0.0	17.695652	36
GTATACG	95	3.6098245E-6	17.526316	1
TACACGC	65	0.0015806376	17.076923	5
CGTCGCG	80	3.3845584E-4	16.1875	15
GTATCAA	4890	0.0	14.9059305	2
CGACCAA	250	0.0	14.799999	12
GAGTAGA	390	0.0	14.705129	1
GACGGTT	140	6.0025013E-7	14.535714	11
GTATTAG	715	0.0	14.489511	1
TTGTACG	90	8.278817E-4	14.388889	9
CCGTGCG	315	0.0	14.095238	9
TATTAGA	530	0.0	13.962264	2
AGTGTCG	80	0.006301357	13.875	5
TCGTTGT	255	1.8189894E-12	13.784313	34
GTGCGAG	310	0.0	13.725807	11
TAATACG	150	1.307144E-6	13.566667	4
CTAGACT	205	1.4260877E-9	13.536586	4
>>END_MODULE