FastQCFastQC Report
Fri 10 Feb 2017
ERR1630024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80668
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7380.9148609113898944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.834283730847424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4830.598750433877126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.471066593940596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.30247433926711953No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.29503644567858384No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.2516487330787921No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.22065750979322657No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG1340.16611295681063123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.14255962711360143No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG1130.1400803292507562No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC1030.12768383993652996No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.12644419100510737No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG950.117766648485149No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT900.1115684038280359No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC890.11032875489661328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1250.035.521
TTCACCA358.797791E-426.4285710
GTATCAA2450.018.122452
TCTTATA1102.412268E-413.45454537
TCAACGC3800.012.1710535
TATCAAC3650.012.1643843
CAACGCA3850.012.0129886
CTCTTAT1552.6469557E-511.93548437
ATCAACG3750.011.8399994
AACGCAG3950.011.708867
GTCTCTT3500.011.62857137
TCTCTTA2451.8981154E-811.32653137
GAGTACG2707.9257006E-810.2777781
AGTACGG2751.03667844E-710.0909092
GCCCAGG1500.00324489279.8666661
CGCAGAG4850.09.5360829
ACGCAGA4900.09.4387768
AGAGTAC5150.08.98058312
CAGAGTA5150.08.98058311
GCAGAGT5301.8189894E-128.72641610
Produced by FastQC (version 0.11.5)