FastQCFastQC Report
Fri 10 Feb 2017
ERR1630023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880487
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33200.377064056595952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.30244625985392176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21500.24418304869918578No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16830.1911442190515022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.161728679696577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.026.641
CGATTGT555.1417784E-420.181829
TCGATTG555.1417784E-420.181828
ATCGTAC500.00703360518.525
GATAGCG500.00703360518.57
GTATCAA11350.017.6035232
TAAGACT953.6044494E-617.5263164
AGTCGCA650.001579537317.07692327
TTAGGCA1452.9758667E-916.5862064
AAATCGG904.4440545E-516.44444526
GGGCTAT803.3814408E-416.18751
GAGTCGC700.00259184815.85714226
TACTCGA700.00259184815.8571425
CGAATTA957.056678E-515.57894815
AGAGCGC1201.9341514E-615.41666610
CGTCAAG855.3623796E-415.23529419
GAAATCG1101.4510211E-515.13636525
GCCTTAC750.00410339214.81
GTGTAAC750.00410339214.81
CTTAGGC1752.233719E-914.83
Produced by FastQC (version 0.11.5)