##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630023.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880487
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65878882936375	34.0	31.0	34.0	31.0	34.0
2	32.91063241138143	34.0	31.0	34.0	31.0	34.0
3	33.042713861760596	34.0	31.0	34.0	31.0	34.0
4	36.40765394605485	37.0	37.0	37.0	35.0	37.0
5	36.33221955576857	37.0	37.0	37.0	35.0	37.0
6	36.38017256359265	37.0	37.0	37.0	35.0	37.0
7	36.31062468838268	37.0	37.0	37.0	35.0	37.0
8	36.31110283286409	37.0	37.0	37.0	35.0	37.0
9	38.04917619453779	39.0	38.0	39.0	35.0	39.0
10	38.00643166792923	39.0	38.0	39.0	35.0	39.0
11	38.05515243268782	39.0	38.0	39.0	35.0	39.0
12	37.97670834435943	39.0	38.0	39.0	35.0	39.0
13	37.99069265077168	39.0	38.0	39.0	35.0	39.0
14	39.386059078668964	41.0	39.0	41.0	36.0	41.0
15	39.37119457754629	41.0	39.0	41.0	36.0	41.0
16	39.28108762537096	40.0	39.0	41.0	36.0	41.0
17	39.19739644083331	40.0	39.0	41.0	36.0	41.0
18	39.04701716209325	40.0	39.0	41.0	36.0	41.0
19	39.078072702947345	40.0	39.0	41.0	36.0	41.0
20	39.306104462643965	40.0	39.0	41.0	36.0	41.0
21	39.31488937372159	41.0	39.0	41.0	36.0	41.0
22	39.33038080062511	41.0	39.0	41.0	36.0	41.0
23	39.254774914337176	41.0	39.0	41.0	36.0	41.0
24	39.20114209522685	41.0	39.0	41.0	36.0	41.0
25	39.17243752605092	41.0	39.0	41.0	36.0	41.0
26	38.99994207750938	40.0	39.0	41.0	35.0	41.0
27	38.902425589474916	40.0	39.0	41.0	35.0	41.0
28	38.84914030530831	40.0	39.0	41.0	35.0	41.0
29	38.723658611654685	40.0	38.0	41.0	35.0	41.0
30	38.627030268476425	40.0	38.0	41.0	35.0	41.0
31	38.494349149959056	40.0	38.0	41.0	35.0	41.0
32	38.398041084081875	40.0	38.0	41.0	34.0	41.0
33	38.30883817705429	40.0	38.0	41.0	34.0	41.0
34	38.224871008884854	40.0	38.0	41.0	34.0	41.0
35	38.058569859634495	40.0	38.0	41.0	34.0	41.0
36	38.03688640491001	40.0	38.0	41.0	34.0	41.0
37	37.97840853981944	40.0	38.0	41.0	33.0	41.0
38	37.86729162383999	40.0	38.0	41.0	33.0	41.0
39	37.81468437353419	40.0	38.0	41.0	33.0	41.0
40	37.73461050532262	40.0	38.0	41.0	33.0	41.0
41	37.61134008792861	40.0	37.0	41.0	33.0	41.0
42	37.5120745678244	40.0	37.0	41.0	33.0	41.0
43	37.19465704774744	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	3.0
16	6.0
17	19.0
18	36.0
19	91.0
20	164.0
21	291.0
22	532.0
23	823.0
24	1323.0
25	1907.0
26	2661.0
27	3725.0
28	4880.0
29	6430.0
30	8248.0
31	10575.0
32	13432.0
33	17590.0
34	23726.0
35	33277.0
36	49630.0
37	87063.0
38	194633.0
39	419421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.171563010016044	18.792441001400363	12.152138532425806	25.883857456157784
2	19.233787665235262	20.775775224392863	34.956904531242365	25.033532579129503
3	19.248097927624144	22.799655190820534	30.814310716682925	27.137936164872396
4	14.620545220997016	15.812612792693134	35.25151421883571	34.31532776747414
5	14.486301330967976	36.35192796713637	34.494546767868236	14.667223934027419
6	34.16586502696803	35.42993820465265	15.337194075551372	15.067002692827947
7	28.801901674868567	30.479609579698508	21.837346831923696	18.88114191350923
8	26.60641213328533	33.94564598909467	19.894558352366364	19.553383525253636
9	26.0874947614218	14.47426253879955	20.114550243217675	39.32369245656097
10	17.034777344810315	26.748833316108016	33.70044077879628	22.515948560285388
11	34.729076068130475	22.091865070125966	22.07710051369299	21.101958348050566
12	21.111498522976486	25.307358314205665	30.315836576803516	23.26530658601433
13	29.735475935476618	20.136810651378156	25.145743208020104	24.98197020512512
14	21.72967914347401	21.402360284706077	26.41140641485905	30.456554156960863
15	24.686679076465637	28.053679384249854	22.962406032116316	24.297235507168192
16	23.574907977062693	27.064226956218544	24.61092554461338	24.74993952210538
17	23.20840625699187	26.89352596915116	26.58029022575007	23.3177775481069
18	22.93935060937867	25.848308947207627	27.04741807658716	24.16492236682654
19	23.91426562799905	25.54256905553404	27.548617980731116	22.994547335735792
20	23.936866756692606	25.89419264566087	27.04855381169739	23.12038678594914
21	24.18979496574055	25.646375244608947	26.661495286131426	23.502334503519076
22	24.079515086537338	25.90645858485134	26.91783070051006	23.096195628101267
23	23.96844019275696	25.776076194197074	26.553714024170716	23.701769588875248
24	23.888257293974814	25.744957052176808	26.746788992909604	23.619996660938774
25	23.997060717534726	25.3134912838009	27.155994353125028	23.533453645539343
26	24.063387647972085	25.887491808510514	26.962237943320005	23.08688260019739
27	23.76219069673942	26.216287122921745	26.37233712706718	23.649185053271655
28	23.422492325269992	25.716222953888018	27.098299009525412	23.762985711316578
29	23.31028169637939	25.744162037599644	27.527152587147796	23.41840367887317
30	23.361049055806614	26.074774528187245	26.974390308999453	23.58978610700669
31	23.288475582262997	25.839450213347842	27.20755672712942	23.664517477259743
32	23.353553204079105	25.513153516179116	27.572014124001832	23.561279155739946
33	23.53243148394014	25.80333383684257	27.256961204424368	23.407273474792927
34	23.9492462693941	25.532915307097092	26.82753975924687	23.690298664261935
35	23.582971696345318	25.433197764419006	27.56951550675933	23.414315032476345
36	23.767415078246472	25.367438701536766	26.833899875864155	24.031246344352613
37	23.031345153307203	24.48599468248821	28.158848455457036	24.323811708747545
38	23.282910480222878	24.55129945132637	28.48423656453758	23.681553503913175
39	22.339455324155836	24.89258785195011	28.824729950584167	23.943226873309882
40	22.547749143371792	24.03783360799194	29.413267884704712	24.00114936393155
41	21.833031038504828	24.306434961561045	29.497766576905736	24.36276742302839
42	21.52842688194147	24.855790034378703	29.09798781810521	24.51779526557462
43	21.710825940644213	23.960376473474337	29.778861016687358	24.549936569194095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1024.0
1	799.5
2	575.0
3	982.5
4	1390.0
5	1390.0
6	1432.5
7	1475.0
8	1310.0
9	1145.0
10	1515.0
11	1885.0
12	1885.0
13	2980.0
14	4075.0
15	5299.5
16	6524.0
17	6761.0
18	6998.0
19	6998.0
20	9169.5
21	11341.0
22	11291.5
23	11242.0
24	13115.5
25	14989.0
26	14989.0
27	16593.5
28	18198.0
29	20589.5
30	22981.0
31	26236.0
32	29491.0
33	29491.0
34	32855.5
35	36220.0
36	39661.5
37	43103.0
38	44636.5
39	46170.0
40	46170.0
41	49071.0
42	51972.0
43	53055.0
44	54138.0
45	54339.5
46	54541.0
47	54541.0
48	54300.5
49	54060.0
50	56371.0
51	58682.0
52	59442.0
53	60202.0
54	60202.0
55	58453.0
56	56704.0
57	53081.5
58	49459.0
59	45688.0
60	41917.0
61	41917.0
62	39982.5
63	38048.0
64	32959.5
65	27871.0
66	24234.0
67	20597.0
68	20597.0
69	17504.0
70	14411.0
71	12685.0
72	10959.0
73	10999.0
74	11039.0
75	11039.0
76	9994.0
77	8949.0
78	6849.0
79	4749.0
80	3304.0
81	1859.0
82	1859.0
83	1299.0
84	739.0
85	566.0
86	393.0
87	295.0
88	197.0
89	197.0
90	156.0
91	115.0
92	78.0
93	41.0
94	24.5
95	8.0
96	8.0
97	8.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	880487.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3649496817831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.80473911606425	55.228177156640015
2	8.910573562367064	11.470572379551658
3	2.2935425391455375	4.428712503753947
4	0.9086382131767967	2.339378114802793
5	0.45690793461462254	1.4704428110338812
6	0.28705573153837866	1.108579662980111
7	0.197561903505522	0.8901243384719149
8	0.14404753144260274	0.7417289690470555
9	0.10266650911658552	0.5947312223964017
>10	0.7639372346199576	9.795051772955254
>50	0.07440048755914518	3.319182222959115
>100	0.05202306797028383	6.118771712859626
>500	0.003018403224958595	1.2100176397022748
>1k	8.877656543995868E-4	1.2845294928460116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3320	0.377064056595952	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2663	0.30244625985392176	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2150	0.24418304869918578	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1683	0.1911442190515022	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1424	0.161728679696577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1357351102287711E-4	0.0
5	0.0	0.0	0.0	1.1357351102287711E-4	0.0
6	0.0	0.0	0.0	1.1357351102287711E-4	0.0
7	0.0	0.0	0.0	1.1357351102287711E-4	0.0
8	0.0	0.0	0.0	1.1357351102287711E-4	0.0
9	0.0	0.0	0.0	4.5429404409150844E-4	0.0
10	0.0	0.0	0.0	4.5429404409150844E-4	0.0
11	0.0	0.0	0.0	4.5429404409150844E-4	0.0
12	0.0	0.0	0.0	7.950145771601398E-4	0.0
13	0.0	0.0	0.0	7.950145771601398E-4	0.0
14	0.0	0.0	0.0	7.950145771601398E-4	0.0
15	0.0	0.0	0.0	9.085880881830169E-4	0.0
16	0.0	0.0	0.0	9.085880881830169E-4	0.0
17	0.0	0.0	0.0	9.085880881830169E-4	0.0
18	0.0	0.0	0.0	9.085880881830169E-4	0.0
19	0.0	0.0	0.0	0.001022161599205894	0.0
20	0.0	0.0	0.0	0.0011357351102287711	0.0
21	0.0	0.0	0.0	0.0015900291543202796	1.1357351102287711E-4
22	0.0	0.0	0.0	0.0019307496873889108	1.1357351102287711E-4
23	0.0	1.1357351102287711E-4	0.0	0.0036343523527320675	1.1357351102287711E-4
24	0.0	1.1357351102287711E-4	0.0	0.00783657226057852	1.1357351102287711E-4
25	0.0	1.1357351102287711E-4	0.0	0.014878129943996902	1.1357351102287711E-4
26	0.0	1.1357351102287711E-4	0.0	0.03032412744310819	1.1357351102287711E-4
27	0.0	1.1357351102287711E-4	0.0	0.09415244063796513	1.1357351102287711E-4
28	0.0	1.1357351102287711E-4	0.0	0.20761237814981937	1.1357351102287711E-4
29	0.0	2.2714702204575422E-4	0.0	0.3303853435655495	1.1357351102287711E-4
30	0.0	2.2714702204575422E-4	0.0	0.4644020865725445	1.1357351102287711E-4
31	0.0	2.2714702204575422E-4	0.0	0.7100615909150277	1.1357351102287711E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	750	0.0	26.64	1
CGATTGT	55	5.1417784E-4	20.18182	9
TCGATTG	55	5.1417784E-4	20.18182	8
ATCGTAC	50	0.007033605	18.5	25
GATAGCG	50	0.007033605	18.5	7
GTATCAA	1135	0.0	17.603523	2
TAAGACT	95	3.6044494E-6	17.526316	4
AGTCGCA	65	0.0015795373	17.076923	27
TTAGGCA	145	2.9758667E-9	16.586206	4
AAATCGG	90	4.4440545E-5	16.444445	26
GGGCTAT	80	3.3814408E-4	16.1875	1
GAGTCGC	70	0.002591848	15.857142	26
TACTCGA	70	0.002591848	15.857142	5
CGAATTA	95	7.056678E-5	15.578948	15
AGAGCGC	120	1.9341514E-6	15.416666	10
CGTCAAG	85	5.3623796E-4	15.235294	19
GAAATCG	110	1.4510211E-5	15.136365	25
GCCTTAC	75	0.004103392	14.8	1
GTGTAAC	75	0.004103392	14.8	1
CTTAGGC	175	2.233719E-9	14.8	3
>>END_MODULE