FastQCFastQC Report
Fri 10 Feb 2017
ERR1630022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386434
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115780.3418935670974246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100470.29668376823525866No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT90990.2686897190377843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85790.25333433340203887No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA84810.25044043380145603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59010.17425409737794978No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA47200.13937965423215098No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG47130.13917294711782363No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC44010.12995971573637638No Hit
GATTAACACTGCTTGGGGCTCCAATGCCCCAGGACTTGTAGGA39790.11749822970121374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27350.029.2212071
TCTAGAC25700.023.0350193
TAGACAT25800.022.7306215
CTAGACA26150.022.5678774
ACATGTA20950.021.8997638
CATGTAT21300.021.5399069
GACATGT21400.021.3528047
GTTCTAG27400.021.1332111
GCCTCTA21500.021.08139425
TTCTAGA29150.020.8164672
CTCTAGT27200.020.67647227
AGACATG22400.020.5647326
CCTCTAG27800.020.3633126
TAGAACT20200.020.2400994
TTTGAAC27900.020.15770735
ATGTATT24000.019.65624810
GTCTAGA20450.019.4498791
CTAGAAC20850.019.2541963
GTATCAA41300.018.8583552
ACTGATC16500.018.7242438
Produced by FastQC (version 0.11.5)