##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3386434
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11561335611442	31.0	31.0	34.0	31.0	34.0
2	32.394273445163854	33.0	31.0	34.0	31.0	34.0
3	32.55913801952142	34.0	31.0	34.0	31.0	34.0
4	36.140799436811704	37.0	35.0	37.0	35.0	37.0
5	35.98917031898451	37.0	35.0	37.0	35.0	37.0
6	36.08068900796531	37.0	35.0	37.0	35.0	37.0
7	36.01945084416233	37.0	35.0	37.0	35.0	37.0
8	36.003891113779275	37.0	35.0	37.0	35.0	37.0
9	37.65098064807996	39.0	37.0	39.0	35.0	39.0
10	37.50336755418827	39.0	37.0	39.0	35.0	39.0
11	37.63273254402714	39.0	37.0	39.0	35.0	39.0
12	37.49968787225737	39.0	37.0	39.0	35.0	39.0
13	37.55186015732183	39.0	37.0	39.0	35.0	39.0
14	38.77315518329901	40.0	38.0	41.0	35.0	41.0
15	38.74008765562831	40.0	38.0	41.0	35.0	41.0
16	38.65813389541919	40.0	38.0	41.0	35.0	41.0
17	38.565681480873394	40.0	38.0	41.0	34.0	41.0
18	38.46337327111646	40.0	38.0	41.0	34.0	41.0
19	38.471970515297215	40.0	38.0	41.0	34.0	41.0
20	38.65982446431851	40.0	38.0	41.0	35.0	41.0
21	38.626989925095245	40.0	38.0	41.0	35.0	41.0
22	38.650770988006855	40.0	38.0	41.0	35.0	41.0
23	38.584390837087035	40.0	38.0	41.0	35.0	41.0
24	38.512595845659476	40.0	38.0	41.0	34.0	41.0
25	38.48245735779879	40.0	38.0	41.0	34.0	41.0
26	38.31100296063647	40.0	38.0	41.0	34.0	41.0
27	38.16926064408756	40.0	38.0	41.0	34.0	41.0
28	38.1160120055492	40.0	38.0	41.0	34.0	41.0
29	37.999707952377044	40.0	38.0	41.0	34.0	41.0
30	37.87514772176277	40.0	37.0	41.0	33.0	41.0
31	37.73068425370168	40.0	37.0	41.0	33.0	41.0
32	37.60855371756839	40.0	37.0	41.0	33.0	41.0
33	37.51074079695633	40.0	37.0	40.0	33.0	41.0
34	37.456438838022535	39.0	37.0	40.0	33.0	41.0
35	37.299243983494144	39.0	37.0	40.0	32.0	41.0
36	37.274194329492325	39.0	37.0	40.0	32.0	41.0
37	37.232071258438815	39.0	37.0	40.0	32.0	41.0
38	37.10643910378882	39.0	36.0	40.0	31.0	41.0
39	37.058260104877284	39.0	36.0	40.0	31.0	41.0
40	36.96709311328672	39.0	36.0	40.0	31.0	41.0
41	36.82497458978973	39.0	36.0	40.0	31.0	41.0
42	36.6940897120688	39.0	36.0	40.0	30.0	41.0
43	36.378846302629846	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	4.0
15	23.0
16	30.0
17	55.0
18	136.0
19	243.0
20	522.0
21	1070.0
22	2019.0
23	3465.0
24	5561.0
25	8456.0
26	12193.0
27	17226.0
28	23901.0
29	31502.0
30	41151.0
31	53199.0
32	68903.0
33	91284.0
34	125845.0
35	184247.0
36	300502.0
37	552016.0
38	1055505.0
39	807370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7338704962211	18.834177781111343	10.991355508478831	25.440596214188734
2	19.761672603098127	21.92580750134212	33.76141392390934	24.551105971650415
3	20.338296863308127	21.969570350403995	30.379626474338494	27.312506311949384
4	14.911201576643752	14.973627125170609	34.83451914314586	35.28065215503979
5	14.719820318364391	36.40286507872293	34.30868577388486	14.568628829027821
6	35.80459563068408	35.17035914475227	14.142723584750211	14.882321639813444
7	30.130426283223	30.260651765249225	20.643662330345137	18.965259621182636
8	26.846618005843315	33.69411599340191	19.906397112715027	19.552868888039747
9	26.415574613295284	13.303522230168962	19.482086466176515	40.79881669035924
10	15.887863162252684	27.32611945190723	34.20793082044416	22.57808656539593
11	35.72953141859549	21.536135061247318	21.55476823112454	21.179565289032652
12	21.959707468091803	25.470509686590674	29.992582167554428	22.5772006777631
13	30.751640220952186	20.084696763616243	25.263861631438857	23.89980138399272
14	22.915905049382328	20.49409496833542	25.505088833858863	31.084911148423384
15	24.924419020125598	27.9817648889658	23.071142092242162	24.022673998666445
16	24.676902015512482	26.202754874301405	24.345845807123364	24.774497303062752
17	23.103536050016036	26.92413317371607	25.716904566868866	24.25542620939903
18	24.25873352322827	24.739416152802622	26.660374895834376	24.34147542813473
19	24.5513422083525	24.865625610893346	26.682728793769495	23.900303386984657
20	24.237826575093447	25.85445338665983	27.064221538054483	22.84349850019224
21	24.187478627960857	25.119521006462847	27.748067731424857	22.944932634151442
22	24.026424256312097	24.912223300380283	26.783749513500045	24.27760292980758
23	23.774979816526766	25.630264756377947	26.84328116242632	23.75147426466897
24	23.73186661839563	25.442367989454397	26.187606195779985	24.638159196369987
25	24.807186556714232	25.83180419284711	26.414570607311404	22.946438643127255
26	25.057272635462553	24.65818025687198	25.981460143620104	24.303086964045363
27	23.81785677795581	25.065422801684605	26.556962279495185	24.559758140864403
28	23.614781802923073	24.550279143193105	27.450291368442436	24.384647685441383
29	23.73966242956455	25.176158755788535	26.163952995983387	24.92022581866353
30	23.0952972950307	25.574129010044196	27.701883456166577	23.62869023875853
31	24.858331802716368	25.23814136049898	26.62632728114589	23.277199555638763
32	24.143420483021373	25.279453253776683	26.937834902437196	23.639291360764744
33	23.94188104655221	24.967916102897618	27.22084647153909	23.869356379011077
34	24.33976271204459	24.278459287852648	26.881167623523744	24.500610376579022
35	23.16147310120321	25.342262686944437	27.48980195686672	24.006462254985628
36	24.271372186789996	23.79500087702876	28.01085153291043	23.922775403270816
37	24.153696779562218	24.29712198731763	27.343246612808635	24.205934620311513
38	23.728825070856246	24.07517760570559	28.30130455812811	23.894692765310058
39	23.68538704725974	24.21278548467208	28.592997826031745	23.50882964203643
40	23.205235950265088	25.053876732870034	27.867160558865166	23.873726757999712
41	24.17909222503672	23.45198518559641	28.161895374308195	24.20702721505867
42	23.039220607872473	23.997396671542987	27.987523158579204	24.975859562005343
43	22.0694098866241	24.168579691793788	28.622645532143842	25.13936488943827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2307.0
1	2010.0
2	1713.0
3	2967.0
4	4221.0
5	4221.0
6	4957.0
7	5693.0
8	4929.5
9	4166.0
10	5816.0
11	7466.0
12	7466.0
13	11382.0
14	15298.0
15	19046.0
16	22794.0
17	22704.5
18	22615.0
19	22615.0
20	28420.0
21	34225.0
22	32202.0
23	30179.0
24	34886.0
25	39593.0
26	39593.0
27	44772.0
28	49951.0
29	57956.5
30	65962.0
31	73345.0
32	80728.0
33	80728.0
34	96068.5
35	111409.0
36	130044.5
37	148680.0
38	153834.0
39	158988.0
40	158988.0
41	170978.0
42	182968.0
43	200284.0
44	217600.0
45	236161.5
46	254723.0
47	254723.0
48	254059.0
49	253395.0
50	262278.5
51	271162.0
52	266470.0
53	261778.0
54	261778.0
55	249922.0
56	238066.0
57	221800.5
58	205535.0
59	197551.5
60	189568.0
61	189568.0
62	177209.0
63	164850.0
64	145719.5
65	126589.0
66	110461.5
67	94334.0
68	94334.0
69	74455.0
70	54576.0
71	44279.0
72	33982.0
73	25393.0
74	16804.0
75	16804.0
76	12165.0
77	7526.0
78	5513.5
79	3501.0
80	2643.0
81	1785.0
82	1785.0
83	1312.5
84	840.0
85	649.5
86	459.0
87	329.5
88	200.0
89	200.0
90	168.0
91	136.0
92	86.5
93	37.0
94	27.0
95	17.0
96	17.0
97	12.5
98	8.0
99	7.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3386434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.294879911167065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24614160751553	30.30065094497965
2	9.788022165142339	7.3008622243365
3	2.97146650851837	3.324614597857425
4	1.4315048913157868	2.135512120554821
5	0.8580776255048624	1.6000950998831618
6	0.5526580136076038	1.2366788549663834
7	0.4137022446318565	1.0800282872767744
8	0.310264051063609	0.9257008420135604
9	0.23621309579327557	0.7928585136949693
>10	1.716390178436073	12.577718614994504
>50	0.22237878420725138	5.840539085692727
>100	0.21201286939301361	16.540014751214215
>500	0.02781835296108206	7.053626370858798
>1k	0.012869985134289877	7.690604625279182
>5k	3.1975118346061807E-4	0.9557953213876766
>10k+	1.5987559173030904E-4	0.6446997450096228
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11578	0.3418935670974246	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10047	0.29668376823525866	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	9099	0.2686897190377843	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8579	0.25333433340203887	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	8481	0.25044043380145603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5901	0.17425409737794978	No Hit
GTGTGAGCCTGACCTCAAGCACAGGATTCCAGAAATGGAAGGA	4720	0.13937965423215098	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	4713	0.13917294711782363	No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC	4401	0.12995971573637638	No Hit
GATTAACACTGCTTGGGGCTCCAATGCCCCAGGACTTGTAGGA	3979	0.11749822970121374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.952958776104894E-5	0.0
5	0.0	0.0	0.0	2.952958776104894E-5	0.0
6	0.0	0.0	0.0	2.952958776104894E-5	0.0
7	0.0	0.0	0.0	2.952958776104894E-5	0.0
8	0.0	0.0	0.0	2.952958776104894E-5	0.0
9	0.0	0.0	0.0	1.1811835104419576E-4	0.0
10	0.0	0.0	0.0	1.476479388052447E-4	0.0
11	0.0	2.952958776104894E-5	0.0	2.0670711432734258E-4	0.0
12	0.0	2.952958776104894E-5	0.0	2.362367020883915E-4	0.0
13	0.0	2.952958776104894E-5	0.0	2.362367020883915E-4	0.0
14	0.0	2.952958776104894E-5	0.0	2.657662898494404E-4	0.0
15	0.0	2.952958776104894E-5	0.0	2.952958776104894E-4	0.0
16	0.0	2.952958776104894E-5	0.0	3.5435505313258724E-4	0.0
17	0.0	2.952958776104894E-5	0.0	3.838846408936362E-4	0.0
18	0.0	2.952958776104894E-5	0.0	4.72473404176783E-4	2.952958776104894E-5
19	0.0	2.952958776104894E-5	0.0	5.315325796988808E-4	2.952958776104894E-5
20	0.0	2.952958776104894E-5	0.0	7.382396940262234E-4	2.952958776104894E-5
21	0.0	2.952958776104894E-5	0.0	0.0010630651593977617	5.905917552209788E-5
22	0.0	2.952958776104894E-5	0.0	0.0017127160901408383	5.905917552209788E-5
23	0.0	2.952958776104894E-5	0.0	0.0025395445474502088	5.905917552209788E-5
24	0.0	2.952958776104894E-5	0.0	0.004931441156095172	5.905917552209788E-5
25	0.0	2.952958776104894E-5	0.0	0.008179695809810556	5.905917552209788E-5
26	0.0	2.952958776104894E-5	0.0	0.015325856047984398	5.905917552209788E-5
27	0.0	2.952958776104894E-5	0.0	0.06218931182476906	5.905917552209788E-5
28	0.0	2.952958776104894E-5	0.0	0.1588101229789212	5.905917552209788E-5
29	0.0	2.952958776104894E-5	0.0	0.27621976391685177	5.905917552209788E-5
30	0.0	2.952958776104894E-5	0.0	0.4100773852376866	5.905917552209788E-5
31	0.0	2.952958776104894E-5	0.0	0.6390498087368601	5.905917552209788E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2735	0.0	29.221207	1
TCTAGAC	2570	0.0	23.035019	3
TAGACAT	2580	0.0	22.730621	5
CTAGACA	2615	0.0	22.567877	4
ACATGTA	2095	0.0	21.899763	8
CATGTAT	2130	0.0	21.539906	9
GACATGT	2140	0.0	21.352804	7
GTTCTAG	2740	0.0	21.133211	1
GCCTCTA	2150	0.0	21.081394	25
TTCTAGA	2915	0.0	20.816467	2
CTCTAGT	2720	0.0	20.676472	27
AGACATG	2240	0.0	20.564732	6
CCTCTAG	2780	0.0	20.36331	26
TAGAACT	2020	0.0	20.240099	4
TTTGAAC	2790	0.0	20.157707	35
ATGTATT	2400	0.0	19.656248	10
GTCTAGA	2045	0.0	19.449879	1
CTAGAAC	2085	0.0	19.254196	3
GTATCAA	4130	0.0	18.858355	2
ACTGATC	1650	0.0	18.724243	8
>>END_MODULE