FastQCFastQC Report
Fri 10 Feb 2017
ERR1630021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276330
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74440.583234743365744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66390.5201632806562566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45210.3542187365336551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.29561320348185816No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.1544271465843473No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.12535942898780097No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA15820.1239491354116882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC250.00549656729.611
GGTATCA16350.029.079511
TACGTTA656.9028945E-519.92307719
CGTGCGA851.2455293E-619.58823410
GTATCAA26450.017.9054812
CGTTTAG1451.546141E-1017.86206826
AACCACG1251.6580088E-716.2799997
AATTACG957.060652E-515.57894816
GAGCTAG855.364728E-415.2352931
ATACTTA855.364728E-415.2352936
GCGTTTA1752.237357E-914.825
TTTCGTC2101.364242E-1014.09523817
ATTACGT1051.6558569E-414.09523817
TGCGTTT2009.840733E-1013.87500124
TGCGTTA1601.7835009E-713.87500137
ATTTTCG2151.9645086E-1013.76744215
AATGCGT1753.565765E-813.74285835
CCGTGCG1356.5678505E-613.7037049
CTTATAC9600.013.68229137
ATGCGTT1907.1249815E-913.63157936
Produced by FastQC (version 0.11.5)