##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1276330
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462142235942114	31.0	31.0	33.0	30.0	34.0
2	31.673597737262305	31.0	31.0	34.0	30.0	34.0
3	31.80041290261923	31.0	31.0	34.0	30.0	34.0
4	35.54794214662352	37.0	35.0	37.0	33.0	37.0
5	35.23763368407857	37.0	35.0	37.0	32.0	37.0
6	35.45791840668166	37.0	35.0	37.0	33.0	37.0
7	35.414165615475625	37.0	35.0	37.0	33.0	37.0
8	35.409415276613416	37.0	35.0	37.0	33.0	37.0
9	36.91526721145785	38.0	37.0	39.0	33.0	39.0
10	36.66222685355668	38.0	35.0	39.0	32.0	39.0
11	36.88514020668636	38.0	37.0	39.0	33.0	39.0
12	36.6810621077621	38.0	35.0	39.0	32.0	39.0
13	36.8033149734003	38.0	37.0	39.0	33.0	39.0
14	37.78323160938002	39.0	37.0	40.0	33.0	41.0
15	37.77073092382064	39.0	37.0	40.0	33.0	41.0
16	37.63689092946181	39.0	37.0	40.0	33.0	41.0
17	37.55337491087728	39.0	37.0	40.0	32.0	41.0
18	37.40120815149687	39.0	36.0	40.0	32.0	41.0
19	37.456797223288646	39.0	36.0	40.0	32.0	41.0
20	37.62249966701402	39.0	37.0	40.0	32.0	41.0
21	37.58588452829597	39.0	37.0	40.0	32.0	41.0
22	37.57902815102677	39.0	37.0	40.0	33.0	41.0
23	37.47226579332931	39.0	36.0	40.0	32.0	41.0
24	37.39973282771697	39.0	36.0	40.0	32.0	41.0
25	37.36159848941888	39.0	36.0	40.0	32.0	41.0
26	37.12419123580892	39.0	36.0	40.0	32.0	41.0
27	36.958241990707734	39.0	36.0	40.0	31.0	41.0
28	36.877620207939955	39.0	36.0	40.0	31.0	41.0
29	36.70592715050183	38.0	36.0	40.0	31.0	41.0
30	36.57009785870425	38.0	35.0	40.0	30.0	41.0
31	36.3948978712402	38.0	35.0	40.0	30.0	41.0
32	36.282001519983076	38.0	35.0	40.0	30.0	41.0
33	36.14695102363809	38.0	35.0	40.0	30.0	41.0
34	36.061122123588724	38.0	35.0	40.0	30.0	41.0
35	35.87425509076806	38.0	35.0	40.0	29.0	41.0
36	35.812257801665716	38.0	35.0	40.0	29.0	41.0
37	35.73191180964171	38.0	35.0	40.0	29.0	41.0
38	35.574096040992536	38.0	34.0	40.0	28.0	41.0
39	35.50165082698048	38.0	34.0	40.0	27.0	41.0
40	35.375934907116495	38.0	34.0	40.0	27.0	41.0
41	35.200566467919735	38.0	34.0	40.0	27.0	41.0
42	35.06512030587701	38.0	34.0	40.0	26.0	41.0
43	34.727492889769884	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	9.0
15	16.0
16	35.0
17	98.0
18	156.0
19	265.0
20	452.0
21	854.0
22	1490.0
23	2397.0
24	3607.0
25	5366.0
26	7595.0
27	10599.0
28	14672.0
29	19483.0
30	25599.0
31	33696.0
32	45111.0
33	62067.0
34	86554.0
35	126787.0
36	184769.0
37	246996.0
38	265571.0
39	132083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9815094842243	23.0887779806163	14.337122844405444	22.592589690753957
2	19.05721874436862	24.528844421113664	34.808709346328925	21.605227488188792
3	20.087908299577695	25.72712386295081	32.33967704277107	21.845290794700432
4	15.480557536060424	18.375420149961215	36.19087540056255	29.953146913415807
5	13.071306010201123	38.6661756755698	35.55749688560169	12.705021428627392
6	31.26652198099238	38.39876834361019	15.716938409345547	14.617771266051882
7	25.729866100459915	32.99452335994609	23.362688332954644	17.912922206639347
8	24.988835175855776	36.148331544349816	21.039072967022634	17.823760312771775
9	24.827905008892685	15.312967649432357	21.890263489849804	37.968863851825155
10	15.62660127083121	28.9998668056067	35.58061003032131	19.792921893240774
11	32.663417768132064	23.77660949754374	23.44824614323882	20.11172659108538
12	19.040922018600206	27.992290395117248	31.955920490782162	21.01086709550038
13	28.68325589776938	22.159629562887343	26.774423542500763	22.38269099684251
14	20.559808200073647	23.638557426370923	28.106289125853028	27.695345247702395
15	23.216174500325153	30.1947772127898	24.86794167652566	21.72110661035939
16	21.743828006863428	29.34860106712214	26.82017973408131	22.08739119193312
17	21.080833326804196	29.10877280953985	28.442095696254103	21.368298167401846
18	20.86568520680388	27.63736651179554	29.586940681485196	21.91000759991538
19	22.115675413098494	27.443216096150678	30.38743898521542	20.053669505535403
20	22.232416381343384	27.29372497708273	29.997101063204656	20.47675757836923
21	21.456284816622663	28.088503756865386	29.217443764543656	21.2377676619683
22	21.50588014071596	27.799550273048506	29.454608134259946	21.239961451975585
23	21.464354829863748	27.74211998464347	29.4620513503561	21.33147383513668
24	21.876473952661147	27.891611103711423	29.279496681892613	20.952418261734817
25	21.91698071815283	27.284636418481114	29.629719586627285	21.168663276738776
26	21.493735946032764	28.061003032131186	29.28733164620435	21.157929375631692
27	21.489583414947543	28.02848793023748	28.887435067733268	21.59449358708171
28	21.14641197809344	28.222873394811685	29.54243808419453	21.088276542900346
29	21.477595919550588	28.127600228780956	29.554112181019015	20.840691670649438
30	21.37182390134213	28.44969561163649	29.237109524966115	20.941370962055267
31	21.540589032616957	27.933058064920512	29.66388003102646	20.862472871436072
32	21.00694961334451	27.97803076006989	29.53726700774878	21.47775261883682
33	21.03327509343195	28.035774447047395	29.839539930895615	21.091410528625044
34	21.304521557904305	27.366590145181892	29.616400147297327	21.71248814961648
35	21.280624916753503	27.60892559134393	29.85685520202456	21.25359428987801
36	21.40018647215062	27.35757993622339	29.734473059475214	21.507760532150776
37	21.356937469149827	26.55966717071604	30.110159598223028	21.973235761911106
38	20.95030282137065	27.04418136375389	30.693002593373187	21.31251322150228
39	20.674512077597488	26.874240987832298	30.8341886502707	21.617058284299514
40	20.566467919738628	26.371392978304986	31.378561970650225	21.683577131306166
41	20.18568865418818	26.546504430672318	31.264719939200674	22.003086975938825
42	20.15638588766228	26.35157051859629	31.054821245289226	22.437222348452202
43	19.866413858484876	26.431017056717305	31.264719939200674	22.43784914559714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3491.0
1	2437.5
2	1384.0
3	1825.0
4	2266.0
5	2266.0
6	2871.0
7	3476.0
8	3215.5
9	2955.0
10	3978.5
11	5002.0
12	5002.0
13	7875.5
14	10749.0
15	14440.5
16	18132.0
17	18036.0
18	17940.0
19	17940.0
20	21744.0
21	25548.0
22	25773.0
23	25998.0
24	29717.5
25	33437.0
26	33437.0
27	36617.5
28	39798.0
29	46899.0
30	54000.0
31	56772.0
32	59544.0
33	59544.0
34	62163.5
35	64783.0
36	68851.5
37	72920.0
38	74942.0
39	76964.0
40	76964.0
41	80548.5
42	84133.0
43	81103.5
44	78074.0
45	77826.0
46	77578.0
47	77578.0
48	76144.5
49	74711.0
50	76105.0
51	77499.0
52	78489.5
53	79480.0
54	79480.0
55	73027.0
56	66574.0
57	61049.0
58	55524.0
59	49988.0
60	44452.0
61	44452.0
62	40531.5
63	36611.0
64	32049.0
65	27487.0
66	23642.5
67	19798.0
68	19798.0
69	16634.5
70	13471.0
71	11424.5
72	9378.0
73	7656.5
74	5935.0
75	5935.0
76	4663.5
77	3392.0
78	2714.0
79	2036.0
80	1512.0
81	988.0
82	988.0
83	715.0
84	442.0
85	329.0
86	216.0
87	156.5
88	97.0
89	97.0
90	71.5
91	46.0
92	31.5
93	17.0
94	9.5
95	2.0
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1276330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.27700866648905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.23474750297852	54.56676865098466
2	8.72286660133562	11.039138110586839
3	2.1627447759519005	4.10556059793937
4	0.8479081980526222	2.146123775862516
5	0.4645349330610335	1.4697190492594965
6	0.28600704146558714	1.085860202489691
7	0.20108389555694295	0.8906791181293642
8	0.1420409395623164	0.7190340610944754
9	0.11134757687943722	0.6341167428473434
>10	0.6743882669001122	8.216196560626754
>50	0.08438434493539836	3.7687117337590523
>100	0.06308374951600838	7.472455202335507
>500	0.0034907914494004925	1.359952945890338
>1k	0.0011220401087358727	1.4147013271449291
>5k	2.493422463857495E-4	1.1109819210495755
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7444	0.583234743365744	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6639	0.5201632806562566	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4521	0.3542187365336551	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3773	0.29561320348185816	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.1544271465843473	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.12535942898780097	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1582	0.1239491354116882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.566992862347512E-4	7.83496431173756E-5	0.0	0.0	0.0
6	2.350489293521268E-4	7.83496431173756E-5	0.0	0.0	0.0
7	3.133985724695024E-4	7.83496431173756E-5	0.0	0.0	0.0
8	3.133985724695024E-4	7.83496431173756E-5	0.0	0.0	0.0
9	3.133985724695024E-4	7.83496431173756E-5	0.0	0.0	0.0
10	3.91748215586878E-4	7.83496431173756E-5	0.0	0.0	0.0
11	4.700978587042536E-4	7.83496431173756E-5	0.0	0.0	0.0
12	5.484475018216293E-4	7.83496431173756E-5	0.0	7.83496431173756E-5	0.0
13	5.484475018216293E-4	7.83496431173756E-5	0.0	7.83496431173756E-5	0.0
14	5.484475018216293E-4	7.83496431173756E-5	0.0	7.83496431173756E-5	0.0
15	6.267971449390048E-4	7.83496431173756E-5	0.0	2.350489293521268E-4	0.0
16	6.267971449390048E-4	7.83496431173756E-5	0.0	3.133985724695024E-4	0.0
17	6.267971449390048E-4	7.83496431173756E-5	0.0	3.91748215586878E-4	0.0
18	6.267971449390048E-4	7.83496431173756E-5	0.0	3.91748215586878E-4	0.0
19	6.267971449390048E-4	7.83496431173756E-5	0.0	3.91748215586878E-4	0.0
20	6.267971449390048E-4	7.83496431173756E-5	0.0	6.267971449390048E-4	0.0
21	7.051467880563804E-4	7.83496431173756E-5	0.0	7.051467880563804E-4	0.0
22	7.051467880563804E-4	7.83496431173756E-5	0.0	0.0012535942898780096	0.0
23	7.051467880563804E-4	7.83496431173756E-5	0.0	0.002428838936638644	0.0
24	7.051467880563804E-4	7.83496431173756E-5	0.0	0.0057195239475684185	0.0
25	7.83496431173756E-4	7.83496431173756E-5	0.0	0.01120399896578471	0.0
26	7.83496431173756E-4	7.83496431173756E-5	0.0	0.018803914348170143	0.0
27	8.618460742911316E-4	7.83496431173756E-5	0.0	0.06424670735624799	0.0
28	9.401957174085072E-4	7.83496431173756E-5	0.0	0.14486849012402747	0.0
29	9.401957174085072E-4	7.83496431173756E-5	0.0	0.25032710976001504	0.0
30	0.0010185453605258828	7.83496431173756E-5	0.0	0.36330729513527066	0.0
31	0.0010185453605258828	7.83496431173756E-5	0.0	0.5972593294837542	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGC	25	0.005496567	29.6	11
GGTATCA	1635	0.0	29.07951	1
TACGTTA	65	6.9028945E-5	19.923077	19
CGTGCGA	85	1.2455293E-6	19.588234	10
GTATCAA	2645	0.0	17.905481	2
CGTTTAG	145	1.546141E-10	17.862068	26
AACCACG	125	1.6580088E-7	16.279999	7
AATTACG	95	7.060652E-5	15.578948	16
GAGCTAG	85	5.364728E-4	15.235293	1
ATACTTA	85	5.364728E-4	15.235293	6
GCGTTTA	175	2.237357E-9	14.8	25
TTTCGTC	210	1.364242E-10	14.095238	17
ATTACGT	105	1.6558569E-4	14.095238	17
TGCGTTT	200	9.840733E-10	13.875001	24
TGCGTTA	160	1.7835009E-7	13.875001	37
ATTTTCG	215	1.9645086E-10	13.767442	15
AATGCGT	175	3.565765E-8	13.742858	35
CCGTGCG	135	6.5678505E-6	13.703704	9
CTTATAC	960	0.0	13.682291	37
ATGCGTT	190	7.1249815E-9	13.631579	36
>>END_MODULE