FastQCFastQC Report
Fri 10 Feb 2017
ERR1630020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420851
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52831.25531363831855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49141.1676341508039663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36950.8779829440823474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27900.6629424665736804No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.5574419450114173No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22270.5291659043224324No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.3754297839377832No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.3314712332868402No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.2233569600642508No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.1839130713720533No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.1287866727178978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG200.001840610637.04
GGTATCA12450.030.164661
GCCTTAA250.00549243629.59999819
CGTCCGG250.00549243629.5999989
GTACTTA400.001929164523.1250026
TATAGAG400.001929164523.1250022
GACCATC652.6761409E-622.769237
CCATCGG603.7190657E-521.58333419
TTTAGAC450.0038218420.5555553
GCAACTA450.0038218420.55555518
ATATGAG450.0038218420.55555531
TATTAGA1001.2840246E-820.3499982
ACCATCG555.1356526E-420.18181818
ACCATCC555.1356526E-420.1818188
TCATGGA555.1356526E-420.1818182
ACCTCTA656.88885E-519.92307731
GCTCGGA656.88885E-519.92307711
TGAGCGT656.88885E-519.92307727
TACACTG759.242905E-619.7333345
TTAGGTT609.2235586E-418.54