##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630020.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420851
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.572675364915376	33.0	31.0	34.0	31.0	34.0
2	32.794319129573175	34.0	31.0	34.0	31.0	34.0
3	32.920435023321794	34.0	31.0	34.0	31.0	34.0
4	36.27828851541282	37.0	37.0	37.0	35.0	37.0
5	36.25221277839425	37.0	37.0	37.0	35.0	37.0
6	36.350304502068425	37.0	37.0	37.0	35.0	37.0
7	36.264525924852265	37.0	37.0	37.0	35.0	37.0
8	36.29562719347227	37.0	37.0	37.0	35.0	37.0
9	38.016145856847196	39.0	38.0	39.0	35.0	39.0
10	37.989095903300694	39.0	38.0	39.0	35.0	39.0
11	38.04473554773542	39.0	38.0	39.0	35.0	39.0
12	37.963184119795365	39.0	38.0	39.0	35.0	39.0
13	38.00700960672542	39.0	38.0	39.0	35.0	39.0
14	39.33312264910859	40.0	39.0	41.0	36.0	41.0
15	39.31263558836738	40.0	39.0	41.0	36.0	41.0
16	39.21179467317412	40.0	39.0	41.0	36.0	41.0
17	39.181869592801256	40.0	39.0	41.0	36.0	41.0
18	39.116744405977414	40.0	39.0	41.0	36.0	41.0
19	39.159141834045776	40.0	39.0	41.0	36.0	41.0
20	39.32550475108768	41.0	39.0	41.0	36.0	41.0
21	39.30382249299633	41.0	39.0	41.0	36.0	41.0
22	39.27548229658478	41.0	39.0	41.0	36.0	41.0
23	39.17916554790175	41.0	39.0	41.0	35.0	41.0
24	39.13374804859677	41.0	39.0	41.0	35.0	41.0
25	39.103024585898574	40.0	39.0	41.0	35.0	41.0
26	38.908454536166005	40.0	38.0	41.0	35.0	41.0
27	38.76523282586949	40.0	38.0	41.0	35.0	41.0
28	38.701870733347434	40.0	38.0	41.0	35.0	41.0
29	38.559449781514125	40.0	38.0	41.0	35.0	41.0
30	38.401669474469585	40.0	38.0	41.0	35.0	41.0
31	38.198483548809435	40.0	37.0	41.0	35.0	41.0
32	38.057509664940795	40.0	37.0	41.0	34.0	41.0
33	37.92087936110405	40.0	37.0	41.0	34.0	41.0
34	37.84649911726478	40.0	37.0	41.0	34.0	41.0
35	37.642001563498724	40.0	36.0	41.0	33.0	41.0
36	37.58353906727084	40.0	36.0	41.0	33.0	41.0
37	37.493934908079105	40.0	36.0	41.0	33.0	41.0
38	37.359314816882936	40.0	36.0	41.0	33.0	41.0
39	37.25434655020423	40.0	35.0	41.0	33.0	41.0
40	37.13204673387968	40.0	35.0	41.0	33.0	41.0
41	36.97022461631314	40.0	35.0	41.0	32.0	41.0
42	36.81275795946784	40.0	35.0	41.0	31.0	41.0
43	36.474122670493834	40.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	6.0
15	2.0
16	3.0
17	5.0
18	12.0
19	21.0
20	42.0
21	110.0
22	204.0
23	345.0
24	533.0
25	892.0
26	1313.0
27	1853.0
28	2620.0
29	3408.0
30	4473.0
31	5492.0
32	6969.0
33	9265.0
34	12987.0
35	18663.0
36	31251.0
37	52618.0
38	90288.0
39	177473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50589638613191	20.664558240327338	13.756650215872126	22.07289515766863
2	19.089178830512463	23.25288522541232	35.289686848789714	22.368249095285506
3	19.184461959220723	25.663952325169713	31.66179954425676	23.489786171352804
4	13.189703719368612	17.551342399091364	37.54036464211799	31.71858923942203
5	12.58117481008718	38.526937086997535	35.55296292512077	13.338925177794517
6	30.216870103670896	37.95072365278923	15.63451197692295	16.197894266616927
7	26.02441244050745	32.01845783899765	23.78585295033159	18.17127677016331
8	25.76351250204942	34.50247237145688	20.768395465378482	18.965619661115216
9	24.545029000762742	14.80690315574871	22.448562555393714	38.19950528809484
10	17.49431509013879	27.810792893446852	34.20973218550033	20.48515983091403
11	31.511627630681645	24.139422265837553	23.07752625038315	21.27142385309765
12	19.714340704905062	29.65966577244678	30.479670952427345	20.146322570220814
13	29.536819444411442	21.887081175998155	26.310499440419534	22.265599939170873
14	20.805463216197655	23.645423202035875	29.239089368921544	26.310024212844922
15	23.22294588821222	29.554165250884516	26.205711760219174	21.01717710068409
16	20.632717992828816	27.880651346913755	28.459240918995082	23.027389741262347
17	19.812950426635556	28.905241997761678	28.887896191288604	22.393911384314162
18	20.573789773577822	26.733927209392398	30.007057129482885	22.6852258875469
19	22.126358259811667	26.094033280187052	31.96808371608954	19.81152474391174
20	22.08762721248138	26.855347854703922	31.407077564268587	19.649947368546112
21	20.86225291136293	27.48312348075685	30.87624836343504	20.778375244445183
22	20.69473519131474	27.205352963400347	31.816723733577916	20.28318811170699
23	20.524603719606226	27.726677612741803	31.407552791843194	20.341165875808777
24	20.128263922385834	28.32237537750891	30.823260488866605	20.726100211238656
25	20.550978849996792	27.728103295465615	31.401374833373332	20.31954302116426
26	19.980468146683744	28.27912966821987	31.286369760318973	20.454032424777417
27	19.60717688683168	28.273189323537306	31.398998695500307	20.720635094130703
28	19.887798769635808	27.93268876633298	31.988280888010244	20.191231576020968
29	20.17554906605901	28.105909217276427	31.81078338889536	19.90775832776921
30	20.116620846808015	28.694953795999055	30.861041081047684	20.32738427614524
31	20.53909816063167	28.550484613319206	31.225778244556864	19.68463898149226
32	19.596484266403074	28.255843517064232	31.459590211262416	20.688082005270275
33	19.251944274814605	28.42502453362354	31.56556596039929	20.757465231162573
34	20.10711629531592	26.83728920686894	32.2121130756491	20.84348142216604
35	19.613830072876148	27.678442013919412	31.778943141396837	20.9287847718076
36	20.191231576020968	27.943381386761583	30.87102086011439	20.99436617710306
37	19.409244602008787	27.116485406949252	31.927451758460833	21.546818232581128
38	19.524249675063146	27.287804947594278	32.411708656983116	20.77623672035946
39	18.815685361327404	27.124564275717532	32.946814905988106	21.112935456966955
40	18.725629735939798	26.81946817282126	33.54417596726633	20.910726123972616
41	18.667176744263408	26.317627854038605	33.211991892617576	21.80320350908041
42	17.623101762856688	27.246460148603663	33.11290694331248	22.01753114522717
43	18.087161489458264	26.3373497983847	33.53776039500916	22.037728317147874
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4903.0
1	3246.0
2	1589.0
3	2095.0
4	2601.0
5	2601.0
6	2706.0
7	2811.0
8	2328.0
9	1845.0
10	2438.5
11	3032.0
12	3032.0
13	4912.0
14	6792.0
15	8477.5
16	10163.0
17	9231.5
18	8300.0
19	8300.0
20	9144.5
21	9989.0
22	7543.0
23	5097.0
24	5406.0
25	5715.0
26	5715.0
27	6399.0
28	7083.0
29	8531.5
30	9980.0
31	11736.5
32	13493.0
33	13493.0
34	15327.0
35	17161.0
36	19161.0
37	21161.0
38	22811.0
39	24461.0
40	24461.0
41	26746.5
42	29032.0
43	30520.0
44	32008.0
45	31748.5
46	31489.0
47	31489.0
48	30902.0
49	30315.0
50	30070.0
51	29825.0
52	27824.0
53	25823.0
54	25823.0
55	24997.0
56	24171.0
57	21383.0
58	18595.0
59	16864.0
60	15133.0
61	15133.0
62	12929.5
63	10726.0
64	8842.5
65	6959.0
66	5557.5
67	4156.0
68	4156.0
69	3471.5
70	2787.0
71	2233.0
72	1679.0
73	1288.5
74	898.0
75	898.0
76	717.5
77	537.0
78	400.5
79	264.0
80	202.0
81	140.0
82	140.0
83	103.5
84	67.0
85	50.0
86	33.0
87	24.0
88	15.0
89	15.0
90	14.5
91	14.0
92	9.5
93	5.0
94	4.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	420851.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.3540201055862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69841042033279	47.73095923738626
2	8.747320536968996	9.858933548207158
3	2.268794646814399	3.8356709742607498
4	1.0147392646139508	2.2873854767992934
5	0.5743638744215155	1.6183856663536234
6	0.37535691490634254	1.2691722677641704
7	0.29254934451162445	1.1540432149738904
8	0.23156779508706715	1.043982094411428
9	0.1840832974386638	0.9336450460464958
>10	1.434072804028808	15.927302099290138
>50	0.1451207509493456	5.664019896069289
>100	0.028939035379791245	2.32441380499243
>500	0.0012767221491084373	0.5410521609639828
>1k	0.002979018347919687	4.5440227366066415
>5k	4.2557404970281246E-4	1.267011775874433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5283	1.25531363831855	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4914	1.1676341508039663	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3695	0.8779829440823474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2790	0.6629424665736804	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2346	0.5574419450114173	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2227	0.5291659043224324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1580	0.3754297839377832	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1395	0.3314712332868402	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	940	0.2233569600642508	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	774	0.1839130713720533	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	542	0.1287866727178978	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.3761378730239444E-4	0.0
16	0.0	0.0	0.0	9.504551492095778E-4	0.0
17	0.0	0.0	0.0	0.0011880689365119722	0.0
18	0.0	0.0	0.0	0.0011880689365119722	0.0
19	0.0	0.0	0.0	0.0011880689365119722	0.0
20	0.0	0.0	0.0	0.0023761378730239443	0.0
21	0.0	0.0	0.0	0.003326593022233522	0.0
22	0.0	0.0	0.0	0.004752275746047889	0.0
23	0.0	0.0	0.0	0.006653186044467044	0.0
24	0.0	0.0	0.0	0.011405461790514933	0.0
25	0.0	0.0	0.0	0.019009102984191555	0.0
26	0.0	0.0	0.0	0.03540445430805677	0.0
27	0.0	0.0	0.0	0.10787665943528707	0.0
28	0.0	0.0	0.0	0.25353391105165485	0.0
29	0.0	0.0	0.0	0.43887266514752254	0.0
30	0.0	0.0	0.0	0.6691204250435427	0.0
31	0.0	0.0	0.0	1.0934986491656191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	20	0.0018406106	37.0	4
GGTATCA	1245	0.0	30.16466	1
GCCTTAA	25	0.005492436	29.599998	19
CGTCCGG	25	0.005492436	29.599998	9
GTACTTA	40	0.0019291645	23.125002	6
TATAGAG	40	0.0019291645	23.125002	2
GACCATC	65	2.6761409E-6	22.76923	7
CCATCGG	60	3.7190657E-5	21.583334	19
TTTAGAC	45	0.00382184	20.555555	3
GCAACTA	45	0.00382184	20.555555	18
ATATGAG	45	0.00382184	20.555555	31
TATTAGA	100	1.2840246E-8	20.349998	2
ACCATCG	55	5.1356526E-4	20.181818	18
ACCATCC	55	5.1356526E-4	20.181818	8
TCATGGA	55	5.1356526E-4	20.181818	2
ACCTCTA	65	6.88885E-5	19.923077	31
GCTCGGA	65	6.88885E-5	19.923077	11
TGAGCGT	65	6.88885E-5	19.923077	27
TACACTG	75	9.242905E-6	19.733334	5
TTAGGTT	60	9.2235586E-4	18.5	4
>>END_MODULE