FastQCFastQC Report
Fri 10 Feb 2017
ERR1630019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34205
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2340.6841105101593334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.5525507966671539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1790.523315304780003No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.39175559128782345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.17833650051162112No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.17541295132290602No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.17541295132290602No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.15494810700190031No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.1344832626808946No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.13155971349217951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAC200.001817081537.01
GAAACCA303.5276034E-430.83333226
CTTACCC250.005423020629.63
CGAAACC250.005423020629.625
GGTATCA1051.8189894E-1224.6666661
CTCTTAT902.0308871E-618.537
TACGGGG700.002524206615.8571424
GCTGTCT800.006135415813.87535
AACGCAG1906.2518666E-913.6315797
TCTCTTA1501.2027613E-613.56666737
GTATCAA1959.0058165E-913.2820522
CGCAGAG2001.2843884E-812.9500019
GAGTACG1501.8169729E-512.3333331
ACGCAGA2153.5286575E-812.0465128
AGTACGG1603.5203477E-511.56252
TATCAAC2256.642222E-811.5111113
AGAGTAC2309.010728E-811.2608712
ATCAACG2309.010728E-811.260874
CAACGCA2309.010728E-811.260876
TCAACGC2351.213757E-711.02127555