##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34205
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465692150270428	31.0	31.0	33.0	30.0	34.0
2	31.656716854261074	31.0	31.0	34.0	30.0	34.0
3	31.75915801783365	31.0	31.0	34.0	30.0	34.0
4	35.48133313843005	37.0	35.0	37.0	33.0	37.0
5	35.24361935389563	37.0	35.0	37.0	32.0	37.0
6	35.45303318228329	37.0	35.0	37.0	33.0	37.0
7	35.41011548019296	37.0	35.0	37.0	33.0	37.0
8	35.44250840520392	37.0	35.0	37.0	33.0	37.0
9	36.92366613068265	38.0	37.0	39.0	33.0	39.0
10	36.67867270866832	38.0	35.0	39.0	32.0	39.0
11	36.84402865078205	38.0	37.0	39.0	33.0	39.0
12	36.66645227305949	38.0	35.0	39.0	32.0	39.0
13	36.81338985528431	38.0	37.0	39.0	33.0	39.0
14	37.79766116064903	39.0	37.0	40.0	33.0	41.0
15	37.72629732495249	39.0	37.0	40.0	33.0	41.0
16	37.591141645958196	39.0	37.0	40.0	32.0	41.0
17	37.521502704283	39.0	37.0	40.0	32.0	41.0
18	37.46116064902792	39.0	36.0	40.0	32.0	41.0
19	37.486010817132	39.0	36.0	40.0	32.0	41.0
20	37.59333430784973	39.0	37.0	40.0	33.0	41.0
21	37.560649027919894	39.0	37.0	40.0	32.0	41.0
22	37.590030697266485	39.0	37.0	40.0	33.0	41.0
23	37.46323636895191	39.0	36.0	40.0	32.0	41.0
24	37.36751936851338	39.0	36.0	40.0	32.0	41.0
25	37.309837743020026	39.0	36.0	40.0	32.0	41.0
26	37.043648589387516	39.0	36.0	40.0	32.0	41.0
27	36.795936266627685	38.0	36.0	40.0	31.0	41.0
28	36.75497734249379	38.0	35.0	40.0	31.0	41.0
29	36.583745066510744	38.0	35.0	40.0	31.0	41.0
30	36.40853676363105	38.0	35.0	40.0	30.0	41.0
31	36.187194854553425	38.0	35.0	40.0	30.0	41.0
32	36.04511036398188	38.0	35.0	40.0	30.0	41.0
33	35.87805876333869	38.0	35.0	40.0	30.0	41.0
34	35.7833942406081	38.0	35.0	40.0	30.0	41.0
35	35.49720801052478	38.0	34.0	40.0	29.0	41.0
36	35.45113287531063	38.0	34.0	40.0	28.0	41.0
37	35.33351849144862	38.0	34.0	40.0	27.0	41.0
38	35.15737465282854	38.0	34.0	40.0	26.0	41.0
39	35.067884812161964	38.0	34.0	40.0	26.0	41.0
40	34.88776494664523	38.0	34.0	40.0	25.0	41.0
41	34.694430638795495	38.0	33.0	40.0	24.0	41.0
42	34.50764508112849	38.0	33.0	40.0	24.0	41.0
43	34.2166057593919	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	7.0
19	8.0
20	9.0
21	22.0
22	46.0
23	61.0
24	117.0
25	167.0
26	219.0
27	340.0
28	448.0
29	567.0
30	767.0
31	958.0
32	1249.0
33	1714.0
34	2404.0
35	3400.0
36	4927.0
37	6742.0
38	6894.0
39	3136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79505920187107	22.198508989913755	13.641280514544658	22.365151293670515
2	20.844905715538665	24.271305364712763	33.480485309165324	21.40330361058325
3	20.213419090776203	28.408127466744627	29.197485747697705	22.180967694781465
4	14.737611460312818	18.541148954831165	37.97690396140915	28.744335623446865
5	12.57126151147493	39.17555912878234	35.839789504458416	12.413389855284315
6	29.67110071626955	38.804268381815525	15.784242069872825	15.740388832042099
7	26.615991814062273	29.97514983189592	25.516737319105392	17.892121034936412
8	25.236076596988745	34.053500950153484	21.172343224674755	19.538079228183015
9	25.005116211080253	15.389562929396286	22.230668030989623	37.37465282853384
10	18.187399502996637	26.952199970764507	33.965794474492036	20.89460605174682
11	31.565560590556935	25.391024703990645	23.23052185353019	19.812892851922236
12	20.380061394532962	29.124397017979824	29.20917994445257	21.286361643034642
13	28.586463967256247	22.02601958777956	26.45519660868294	22.932319836281245
14	20.801052477707938	23.294839935681917	28.124543195439262	27.77956439117088
15	23.528723870779125	29.738342347609997	25.130828826195	21.602104955415875
16	21.701505627832187	27.43166203771378	28.431515860254347	22.43531647419968
17	20.85367636310481	29.700336208156703	28.127466744627977	21.31852068411051
18	20.546703698289722	28.259026458120157	28.870048238561612	22.324221605028505
19	22.251132875310628	27.098377430200266	30.773278760415145	19.877210934073965
20	22.02894313696828	27.107148077766407	30.165180529162406	20.698728256102907
21	20.488232714515423	28.332115187838035	29.69448910977927	21.48516298786727
22	21.280514544657215	27.5895336939044	30.019003069726647	21.110948691711737
23	21.213272913316768	28.42566876187692	30.29381669346587	20.067241631340448
24	20.146177459435755	29.31442771524631	29.244262534717148	21.29513229060079
25	21.005700920917995	28.200555474345855	29.95760853676363	20.83613506797252
26	20.00584709837743	29.171173804999267	30.3581347756176	20.4648443210057
27	19.906446425961118	28.78234176290016	29.668177167080835	21.643034644057888
28	20.058470983774303	28.72387077912586	31.273205671685428	19.944452565414412
29	21.257126151147492	28.542610729425522	30.711884227452124	19.488378891974858
30	20.567168542610727	28.814500803976028	29.475222920625637	21.143107732787605
31	21.046630609560005	28.624470106709545	30.176874725917262	20.152024557813185
32	20.333284607513523	28.411051015933342	30.2031866686157	21.052477707937438
33	19.871363835696535	29.04546118988452	29.811431077327878	21.27174389709107
34	20.84782926472738	26.966817716708086	30.50138868586464	21.683964332699897
35	20.640257272328608	28.32334454027189	30.194416021049552	20.841982166349947
36	21.482239438678555	27.478438824733225	29.03669054231837	22.002631194269846
37	20.409296886420115	25.940651951469086	30.98377430200263	22.666276860108173
38	20.081859377284022	26.75339862593188	31.705890951615263	21.458851045168835
39	19.4386785557667	26.595526969741268	32.03625200994007	21.929542464551965
40	19.312965940651953	26.20669492764216	32.45139599473762	22.02894313696828
41	18.99137552989329	25.779856746089752	32.211664961262976	23.017102762753982
42	17.713784534424793	27.051600643180823	32.1590410758661	23.075573746528285
43	18.769185791550942	25.317935974272764	33.01856453734834	22.89431369682795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	96.5
2	72.0
3	70.5
4	69.0
5	69.0
6	98.5
7	128.0
8	106.5
9	85.0
10	134.0
11	183.0
12	183.0
13	292.0
14	401.0
15	492.5
16	584.0
17	526.5
18	469.0
19	469.0
20	489.5
21	510.0
22	398.0
23	286.0
24	287.0
25	288.0
26	288.0
27	341.0
28	394.0
29	558.5
30	723.0
31	935.5
32	1148.0
33	1148.0
34	1351.0
35	1554.0
36	1781.5
37	2009.0
38	2212.5
39	2416.0
40	2416.0
41	2768.5
42	3121.0
43	3324.5
44	3528.0
45	3408.5
46	3289.0
47	3289.0
48	3225.0
49	3161.0
50	3040.0
51	2919.0
52	2509.5
53	2100.0
54	2100.0
55	1906.0
56	1712.0
57	1345.0
58	978.0
59	836.5
60	695.0
61	695.0
62	584.5
63	474.0
64	389.0
65	304.0
66	251.5
67	199.0
68	199.0
69	155.0
70	111.0
71	95.0
72	79.0
73	64.5
74	50.0
75	50.0
76	32.5
77	15.0
78	13.0
79	11.0
80	9.0
81	7.0
82	7.0
83	6.5
84	6.0
85	5.0
86	4.0
87	2.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	34205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.16021049554159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28588353230657	52.15319397748868
2	9.081381572951791	11.65326706621839
3	3.4402624624077283	6.621838912439702
4	2.0049211701449012	5.145446572138576
5	1.212065980133054	3.888320420991083
6	0.8065251070810171	3.104809238415436
7	0.5331267656976214	2.394386785557667
8	0.3645311218445275	1.871071480777664
9	0.2597284243142258	1.4997807338108464
>10	0.9751207509341111	8.832042099108318
>50	0.018226556092226374	0.6841105101593334
>100	0.018226556092226374	2.1517322028943138
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.6841105101593334	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.5525507966671539	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	179	0.523315304780003	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134	0.39175559128782345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61	0.17833650051162112	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.17541295132290602	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.17541295132290602	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.15494810700190031	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.1344832626808946	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.13155971349217951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0029235491887151	0.0
19	0.0	0.0	0.0	0.0029235491887151	0.0
20	0.0	0.0	0.0	0.0029235491887151	0.0
21	0.0	0.0	0.0	0.0029235491887151	0.0
22	0.0	0.0	0.0	0.0087706475661453	0.0
23	0.0	0.0	0.0	0.0116941967548604	0.0
24	0.0	0.0	0.0	0.032159041075866104	0.0
25	0.0	0.0	0.0	0.049700336208156705	0.0
26	0.0	0.0	0.0	0.0818593772840228	0.0
27	0.0	0.0	0.0	0.1812600497003362	0.0
28	0.0	0.0	0.0	0.2982020172489402	0.0
29	0.0	0.0	0.0	0.4414559274959801	0.0
30	0.0	0.0	0.0	0.6490279198947523	0.0
31	0.0	0.0	0.0	1.0320128636164303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTTAC	20	0.0018170815	37.0	1
GAAACCA	30	3.5276034E-4	30.833332	26
CTTACCC	25	0.0054230206	29.6	3
CGAAACC	25	0.0054230206	29.6	25
GGTATCA	105	1.8189894E-12	24.666666	1
CTCTTAT	90	2.0308871E-6	18.5	37
TACGGGG	70	0.0025242066	15.857142	4
GCTGTCT	80	0.0061354158	13.875	35
AACGCAG	190	6.2518666E-9	13.631579	7
TCTCTTA	150	1.2027613E-6	13.566667	37
GTATCAA	195	9.0058165E-9	13.282052	2
CGCAGAG	200	1.2843884E-8	12.950001	9
GAGTACG	150	1.8169729E-5	12.333333	1
ACGCAGA	215	3.5286575E-8	12.046512	8
AGTACGG	160	3.5203477E-5	11.5625	2
TATCAAC	225	6.642222E-8	11.511111	3
AGAGTAC	230	9.010728E-8	11.26087	12
ATCAACG	230	9.010728E-8	11.26087	4
CAACGCA	230	9.010728E-8	11.26087	6
TCAACGC	235	1.213757E-7	11.0212755	5
>>END_MODULE