FastQCFastQC Report
Fri 10 Feb 2017
ERR1630018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284323
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44651.5703970484273169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43101.515881585380008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30841.0846818583090359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.7948706224962455No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.6844328457423423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15110.5314378365450562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.3914561959461598No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.3394027215526004No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.27398416589582975No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.2219306915022703No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.1522915838676435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCA250.005489473729.64
ACTATTG250.005489473729.63
GGTATCA12150.027.5596711
CGGGACC502.6951762E-422.24
CATTATG450.00381883420.5555572
GTATCAA17800.018.915732
ATCATCC609.213527E-418.53
GGAGAGT752.0603795E-417.2666666
CAGAACT650.001575982817.0769234
CTTGCTC1201.9229647E-615.4166663
TCACACC1001.0887732E-414.83
CCTGTAC750.00409431114.83
GTGTAAT750.00409431114.81
ATACACA1651.627268E-814.57575737
TTGCTCT1601.7670027E-713.8754
GTCTTGA950.001241043813.6315791
TTCTGGA950.001241043813.6315792
CCTGAGC950.001241043813.6315793
CAGCCAA850.00938198313.0588239
GGTGTGA1000.001826593312.95000125