##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630018.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284323
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68161914442377	33.0	31.0	34.0	31.0	34.0
2	32.87247953911572	34.0	31.0	34.0	31.0	34.0
3	32.9926105169121	34.0	31.0	34.0	31.0	34.0
4	36.301115280860145	37.0	37.0	37.0	35.0	37.0
5	36.30070377704231	37.0	37.0	37.0	35.0	37.0
6	36.390612085550586	37.0	37.0	37.0	35.0	37.0
7	36.30128410293926	37.0	37.0	37.0	35.0	37.0
8	36.337088452218076	37.0	37.0	37.0	35.0	37.0
9	38.07918458935788	39.0	38.0	39.0	35.0	39.0
10	38.0794343053499	39.0	38.0	39.0	37.0	39.0
11	38.12730943328538	39.0	39.0	39.0	37.0	39.0
12	38.052552906377606	39.0	38.0	39.0	35.0	39.0
13	38.08457634450959	39.0	38.0	39.0	37.0	39.0
14	39.40804999947243	41.0	39.0	41.0	36.0	41.0
15	39.409703048997095	41.0	39.0	41.0	36.0	41.0
16	39.298002623776476	41.0	39.0	41.0	36.0	41.0
17	39.29269879679097	40.0	39.0	41.0	36.0	41.0
18	39.220601217629245	40.0	39.0	41.0	36.0	41.0
19	39.26146319502819	41.0	39.0	41.0	36.0	41.0
20	39.45451124249533	41.0	39.0	41.0	36.0	41.0
21	39.42421823067427	41.0	39.0	41.0	36.0	41.0
22	39.37607228398687	41.0	39.0	41.0	36.0	41.0
23	39.29309623210222	41.0	39.0	41.0	35.0	41.0
24	39.2482599015908	41.0	39.0	41.0	35.0	41.0
25	39.2004129106685	41.0	39.0	41.0	35.0	41.0
26	39.00770954161288	41.0	39.0	41.0	35.0	41.0
27	38.85919183463877	40.0	38.0	41.0	35.0	41.0
28	38.80050857651333	40.0	38.0	41.0	35.0	41.0
29	38.63037812628595	40.0	38.0	41.0	35.0	41.0
30	38.475540142725	40.0	38.0	41.0	35.0	41.0
31	38.241647703492156	40.0	37.0	41.0	35.0	41.0
32	38.05364321563855	40.0	37.0	41.0	35.0	41.0
33	37.902733862543656	40.0	36.0	41.0	34.0	41.0
34	37.77464362714237	40.0	36.0	41.0	34.0	41.0
35	37.57366445908351	40.0	35.0	41.0	33.0	41.0
36	37.4445296370677	40.0	35.0	41.0	33.0	41.0
37	37.320431340412135	40.0	35.0	41.0	33.0	41.0
38	37.137143319393786	40.0	35.0	41.0	33.0	41.0
39	37.002648396366105	40.0	35.0	41.0	33.0	41.0
40	36.847536076926595	40.0	35.0	41.0	32.0	41.0
41	36.64649711771471	40.0	35.0	41.0	31.0	41.0
42	36.44899990503758	40.0	35.0	41.0	30.0	41.0
43	36.079054455671894	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	0.0
16	2.0
17	7.0
18	8.0
19	19.0
20	36.0
21	79.0
22	150.0
23	236.0
24	381.0
25	623.0
26	959.0
27	1398.0
28	1914.0
29	2554.0
30	3074.0
31	3923.0
32	4909.0
33	6307.0
34	8690.0
35	12094.0
36	20879.0
37	35607.0
38	52022.0
39	128449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49622436454314	20.36627356914495	14.247176626583148	20.89032543972876
2	19.001628429638124	24.240388572152092	35.16247366551422	21.595509332695563
3	20.253373803737297	25.112987693573857	32.610094856905704	22.023543645783143
4	13.29544215557658	17.552572250574173	36.53344963298784	32.618535960861415
5	13.017237437702894	38.12600457929889	34.61133991973917	14.245418063259041
6	30.52021820253725	36.92349897827471	15.632924525979256	16.92335829320878
7	24.974061190969426	32.9857239829349	23.246448581366966	18.793766244728708
8	26.174808228669505	34.122811028302316	21.350365605315083	18.352015137713092
9	24.480960034889897	14.505685435226837	22.95487878223007	38.0584757476532
10	16.930744259170027	28.632928043105903	33.74964389092687	20.6866838067972
11	31.148728734573005	23.582685888936176	23.00447026796988	22.264115108520944
12	19.83307716927579	29.405640767718406	31.532095539228273	19.229186523777532
13	30.532879858470825	22.022488507788676	26.21138634581093	21.233245287929574
14	20.26673888500051	24.402528110634737	30.358782089384256	24.971950914980496
15	23.294281503782667	29.35182873000074	26.900391456195944	20.453498310020645
16	19.722287679857065	28.526007393000214	28.95052457943958	22.80118034770314
17	19.208435476553078	28.68709179348839	29.875880600584548	22.228592129373986
18	20.180920994784103	26.037992002054	31.35905290813617	22.42203409502573
19	21.723884455355353	26.236357945013246	32.81057107585387	19.229186523777532
20	22.1332779972074	26.857482511087742	32.426149133204135	18.58309035850072
21	21.287057325647236	27.100164249814473	31.7895492098775	19.82322921466079
22	20.109875036490187	27.612609602459177	33.188662190536824	19.08885317051382
23	20.03636708954253	27.52538486158348	32.14196530002849	20.296282748845503
24	19.819008662682936	27.75681179503593	32.75816588879549	19.66601365348565
25	20.04515990616306	27.854939628521088	32.326262736394874	19.77363772892098
26	19.74550071573527	28.00265894774605	32.29847743587399	19.953362900644688
27	19.35932020976143	28.095511091258885	32.616073972207666	19.929094726772018
28	19.26435779025967	28.108172747192455	32.45041730707681	20.177052155471067
29	19.215118017184682	28.291766758229198	33.002254478181506	19.490860746404618
30	19.42122163876999	28.886512874442094	32.199646177059186	19.492619309728724
31	19.667772216809755	29.160145327673103	32.1289519314301	19.043130524087044
32	18.9122934127735	28.82988713540586	32.695912747122115	19.56190670469853
33	19.011476384253122	28.39939083366453	32.81901217980958	19.77012060227277
34	18.98263594573777	27.807458418770203	33.22735058366717	19.98255505182486
35	18.527519757458947	28.300207862184912	32.85207317030279	20.320199210053357
36	18.922141367388498	29.051114401578488	32.334703840350585	19.69204039068243
37	18.796931658712097	28.073001480710317	32.79193030461834	20.338136555959245
38	18.578869806522864	28.356481888556324	32.96391779771598	20.100730507204833
39	18.206757807141877	27.766659749650923	33.8934943708388	20.1330880723684
40	18.060445338576198	27.369576151067626	34.036289712756265	20.533688797599915
41	17.824446140481072	27.342845988541203	34.140396661543384	20.69231120943434
42	17.10835915490481	27.476848513838135	34.116831913000354	21.2979604182567
43	16.974708342272695	27.225373958490874	34.14954119082874	21.65037650840769
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3705.0
1	2362.5
2	1020.0
3	1466.0
4	1912.0
5	1912.0
6	2073.0
7	2234.0
8	1971.0
9	1708.0
10	2245.0
11	2782.0
12	2782.0
13	4422.0
14	6062.0
15	7590.5
16	9119.0
17	8288.5
18	7458.0
19	7458.0
20	8460.5
21	9463.0
22	7658.5
23	5854.0
24	6169.5
25	6485.0
26	6485.0
27	7195.0
28	7905.0
29	8765.0
30	9625.0
31	10319.0
32	11013.0
33	11013.0
34	11626.5
35	12240.0
36	12814.5
37	13389.0
38	13731.0
39	14073.0
40	14073.0
41	14071.5
42	14070.0
43	13986.5
44	13903.0
45	13890.0
46	13877.0
47	13877.0
48	13768.5
49	13660.0
50	13939.5
51	14219.0
52	14485.0
53	14751.0
54	14751.0
55	14851.0
56	14951.0
57	14897.5
58	14844.0
59	13931.5
60	13019.0
61	13019.0
62	11675.5
63	10332.0
64	8936.0
65	7540.0
66	6176.5
67	4813.0
68	4813.0
69	4066.5
70	3320.0
71	2641.5
72	1963.0
73	1631.5
74	1300.0
75	1300.0
76	1074.5
77	849.0
78	659.0
79	469.0
80	347.0
81	225.0
82	225.0
83	154.0
84	83.0
85	63.5
86	44.0
87	31.0
88	18.0
89	18.0
90	16.5
91	15.0
92	10.5
93	6.0
94	4.5
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	284323.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.42373024965056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3446892829627	65.87862283217724
2	9.198319908440402	13.875431990483985
3	2.0075534540608087	4.542515105425101
4	0.6527387453373629	1.9692796420728247
5	0.25313167088901445	0.9546067431388175
6	0.142597603875583	0.64531459253751
7	0.0817017463958312	0.43135753367591734
8	0.05779931234805108	0.3487551794523762
9	0.04620754598954272	0.3136630935792234
>10	0.19698708191163344	2.523825400811223
>50	0.009371101430284074	0.47673891112019134
>100	0.004216995643627661	0.5958352884719181
>500	0.0014056652145425536	0.8393290688735502
>1k	0.0032798855005992914	6.604724618180118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4465	1.5703970484273169	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4310	1.515881585380008	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3084	1.0846818583090359	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2260	0.7948706224962455	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1946	0.6844328457423423	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1511	0.5314378365450562	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.3914561959461598	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	965	0.3394027215526004	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	779	0.27398416589582975	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	631	0.2219306915022703	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	433	0.1522915838676435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.5171266482134756E-4	0.0
11	3.5171266482134756E-4	0.0	0.0	7.034253296426951E-4	0.0
12	3.5171266482134756E-4	0.0	0.0	7.034253296426951E-4	0.0
13	3.5171266482134756E-4	0.0	0.0	7.034253296426951E-4	0.0
14	3.5171266482134756E-4	0.0	0.0	7.034253296426951E-4	0.0
15	3.5171266482134756E-4	0.0	0.0	7.034253296426951E-4	0.0
16	3.5171266482134756E-4	0.0	0.0	0.0010551379944640426	0.0
17	3.5171266482134756E-4	0.0	0.0	0.0010551379944640426	0.0
18	3.5171266482134756E-4	0.0	0.0	0.0021102759889280853	0.0
19	3.5171266482134756E-4	0.0	0.0	0.003165413983392128	0.0
20	3.5171266482134756E-4	0.0	0.0	0.004923977307498865	0.0
21	3.5171266482134756E-4	0.0	0.0	0.006330827966784256	0.0
22	3.5171266482134756E-4	0.0	0.0	0.008792816620533689	0.0
23	3.5171266482134756E-4	0.0	0.0	0.00984795461499773	0.0
24	3.5171266482134756E-4	0.0	0.0	0.015123644587317945	0.0
25	3.5171266482134756E-4	0.0	0.0	0.024619886537494327	0.0
26	3.5171266482134756E-4	0.0	0.0	0.042205519778561705	0.0
27	3.5171266482134756E-4	0.0	0.0	0.1340025252969334	0.0
28	3.5171266482134756E-4	0.0	0.0	0.3070451563890364	0.0
29	3.5171266482134756E-4	0.0	0.0	0.544451205143446	0.0
30	3.5171266482134756E-4	0.0	0.0	0.8145665317262409	0.0
31	3.5171266482134756E-4	0.0	0.0	1.2007470377000806	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCA	25	0.0054894737	29.6	4
ACTATTG	25	0.0054894737	29.6	3
GGTATCA	1215	0.0	27.559671	1
CGGGACC	50	2.6951762E-4	22.2	4
CATTATG	45	0.003818834	20.555557	2
GTATCAA	1780	0.0	18.91573	2
ATCATCC	60	9.213527E-4	18.5	3
GGAGAGT	75	2.0603795E-4	17.266666	6
CAGAACT	65	0.0015759828	17.076923	4
CTTGCTC	120	1.9229647E-6	15.416666	3
TCACACC	100	1.0887732E-4	14.8	3
CCTGTAC	75	0.004094311	14.8	3
GTGTAAT	75	0.004094311	14.8	1
ATACACA	165	1.627268E-8	14.575757	37
TTGCTCT	160	1.7670027E-7	13.875	4
GTCTTGA	95	0.0012410438	13.631579	1
TTCTGGA	95	0.0012410438	13.631579	2
CCTGAGC	95	0.0012410438	13.631579	3
CAGCCAA	85	0.009381983	13.058823	9
GGTGTGA	100	0.0018265933	12.950001	25
>>END_MODULE