FastQCFastQC Report
Fri 10 Feb 2017
ERR1630017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587970
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23830.40529278704695815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.3743388268108917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14510.24678129836556284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.20987465346871437No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6220.10578771025732604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4350.031.0459791
CGACCAA551.9006098E-523.54545412
GTTTAGG400.001930071423.1251
TAGAGCC555.138988E-420.1818184
GTATCAA7400.018.52
TATACTG801.6144826E-518.55
TAGTCCG609.2295E-418.55
TAAGCTA1054.7874164E-717.6190533
GATATAC650.001578693417.0769233
CCACGAC904.440015E-516.4444459
GTATTAT803.379051E-416.18751
CTTATAT855.3586194E-415.2352933
GGATAAG1101.4493917E-515.1363641
ATACACT1601.092485E-815.031254
TATACTT1508.076677E-814.85
TTGTACT1405.9799095E-714.5357144
TACACCA1152.206106E-514.4782615
CACGACC908.26554E-414.38888910
ACGACCA908.26554E-414.38888911
TTTAGGG1304.434789E-614.2307692
Produced by FastQC (version 0.11.5)