##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630017.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587970
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48182050104597	31.0	31.0	33.0	30.0	34.0
2	31.70012925829549	31.0	31.0	34.0	30.0	34.0
3	31.84146129904587	31.0	31.0	34.0	30.0	34.0
4	35.571445822065755	37.0	35.0	37.0	33.0	37.0
5	35.253711924077756	37.0	35.0	37.0	32.0	37.0
6	35.45993673146589	37.0	35.0	37.0	33.0	37.0
7	35.405277480143546	37.0	35.0	37.0	33.0	37.0
8	35.3937394765039	37.0	35.0	37.0	33.0	37.0
9	36.90612956443356	38.0	37.0	39.0	33.0	39.0
10	36.641644981886834	38.0	35.0	39.0	32.0	39.0
11	36.855567460924874	38.0	37.0	39.0	33.0	39.0
12	36.636396414783064	38.0	35.0	39.0	32.0	39.0
13	36.768355528343285	38.0	37.0	39.0	33.0	39.0
14	37.75117437964522	39.0	37.0	40.0	33.0	41.0
15	37.72786536728064	39.0	37.0	40.0	33.0	41.0
16	37.57366362229365	39.0	37.0	40.0	32.0	41.0
17	37.48068779019338	39.0	36.0	40.0	32.0	41.0
18	37.31742095685154	39.0	36.0	40.0	32.0	41.0
19	37.312407095600115	39.0	36.0	40.0	32.0	41.0
20	37.52829906287736	39.0	37.0	40.0	32.0	41.0
21	37.49102675306563	39.0	36.0	40.0	32.0	41.0
22	37.48560810925728	39.0	36.0	40.0	32.0	41.0
23	37.401648043267514	39.0	36.0	40.0	32.0	41.0
24	37.31659608483426	39.0	36.0	40.0	32.0	41.0
25	37.284478799938775	39.0	36.0	40.0	32.0	41.0
26	37.03281970168546	39.0	36.0	40.0	31.0	41.0
27	36.84962668163342	39.0	36.0	40.0	31.0	41.0
28	36.755790261407896	38.0	36.0	40.0	31.0	41.0
29	36.583954963688626	38.0	35.0	40.0	30.0	41.0
30	36.43112743847475	38.0	35.0	40.0	30.0	41.0
31	36.260207153426194	38.0	35.0	40.0	30.0	41.0
32	36.14788509617838	38.0	35.0	40.0	30.0	41.0
33	36.015837542731774	38.0	35.0	40.0	30.0	41.0
34	35.92813238770685	38.0	35.0	40.0	29.0	41.0
35	35.73252036668538	38.0	34.0	40.0	29.0	41.0
36	35.67805500280627	38.0	34.0	40.0	28.0	41.0
37	35.60160552409137	38.0	34.0	40.0	28.0	41.0
38	35.475036141299725	38.0	34.0	40.0	27.0	41.0
39	35.37704304641393	38.0	34.0	40.0	27.0	41.0
40	35.26642685851319	38.0	34.0	40.0	27.0	41.0
41	35.075957957038625	38.0	34.0	40.0	26.0	41.0
42	34.92534312975152	38.0	33.0	40.0	26.0	41.0
43	34.591414527952104	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	4.0
16	13.0
17	39.0
18	70.0
19	142.0
20	239.0
21	431.0
22	836.0
23	1216.0
24	1801.0
25	2809.0
26	3838.0
27	5189.0
28	6961.0
29	9443.0
30	12328.0
31	16390.0
32	21539.0
33	29527.0
34	41015.0
35	58929.0
36	84472.0
37	110904.0
38	116762.0
39	63067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27931697195435	21.66879262547409	13.255778356038572	23.796112046532986
2	20.47043216490637	23.174821844651937	32.67020426212221	23.684541728319473
3	20.821810636597103	24.332704049526335	30.637957718931236	24.207527594945322
4	16.027008180689492	17.16873309862748	35.11029474292906	31.69396397775397
5	14.383046754086093	37.283875027637464	34.688334438831916	13.644743779444529
6	33.71277446128204	37.02127659574468	14.724900930319574	14.541048012653707
7	28.120992567648006	31.380682687892243	21.901797710767557	18.596527033692194
8	27.038964573022433	33.929622259639096	19.932989778390052	19.098423388948415
9	25.825467285745873	14.550742384815551	20.32994880691192	39.293841522526655
10	16.699491470653268	27.895811010765854	34.14612990458697	21.25856761399391
11	34.81044951273024	22.775821895674948	22.14687824208718	20.266850349507628
12	21.156861744646836	26.195554194941916	30.01122506250319	22.636358997908058
13	30.37297821317414	20.81534772182254	24.947191183223634	23.864482881779683
14	21.460448662346717	21.711822031736315	26.508665408099052	30.319063897817916
15	24.35583448135109	29.28550776400157	22.885351293433338	23.47330646121401
16	23.390479106076842	27.78049900505128	25.115907274180653	23.713114614691225
17	22.953722128680035	27.57130465840094	26.511046481963362	22.96392673095566
18	22.92208786162559	26.633841862680068	27.33370750208344	23.1103627736109
19	24.290354950082488	26.08279333979625	28.19582631766927	21.431025392451996
20	24.366889467149687	26.105753694916405	27.100192186676193	22.427164651257716
21	23.31428474241883	26.298790754630335	27.424188308927327	22.962736194023506
22	23.567018725445177	26.382298416585883	27.678452982295017	22.37222987567393
23	23.4107182339235	26.276510706328555	27.041515723591342	23.271255336156607
24	23.135874279299966	26.368522203513784	27.309556610031123	23.186046907155127
25	23.55970542714764	26.251849584162457	27.254621834447335	22.933823154242564
26	23.324319267989864	26.780447982039902	27.04831879177509	22.846913958195145
27	23.198972736704253	26.53077537969624	27.305984999234656	22.964266884364847
28	23.282650475364388	26.76599146214943	27.104274027586438	22.84708403489974
29	23.38095481061959	26.89950167525554	27.453951732231236	22.265591781893633
30	23.64032178512509	26.654421143935913	26.789972277497153	22.91528479344184
31	23.033147949725326	26.8978009082096	27.50514482031396	22.56390632175111
32	22.790618568974608	26.518359780260898	27.597326394203787	23.093695256560707
33	22.548769495042265	27.03760395938568	27.358198547544944	23.055427998027113
34	23.635389560691873	25.809480075514056	27.364661462319507	23.190468901474564
35	23.02192288722214	25.934316376685885	27.72420361583074	23.31955712026124
36	22.888752827525213	26.010340663639305	27.894110243719915	23.206796265115567
37	22.855417793424834	24.91912852696566	28.87579298263517	23.349660696974336
38	22.828885827508206	25.416432811197847	28.593805806418693	23.160875554875247
39	22.21354831028794	25.432249944725072	29.08464717587632	23.269554569110667
40	22.00027212272735	24.813680970117524	29.56120210214807	23.624844805007058
41	21.45075429018487	24.959776859363572	29.710869602190588	23.878599248260965
42	21.34377604299539	25.17390343044713	29.62157933227886	23.86074119427862
43	21.662159633994932	24.869806282633466	29.827031991428132	23.641002091943466
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1019.0
1	720.5
2	422.0
3	550.0
4	678.0
5	678.0
6	864.0
7	1050.0
8	958.0
9	866.0
10	1178.0
11	1490.0
12	1490.0
13	2404.0
14	3318.0
15	4363.5
16	5409.0
17	5492.0
18	5575.0
19	5575.0
20	7102.0
21	8629.0
22	8499.0
23	8369.0
24	10169.0
25	11969.0
26	11969.0
27	13031.5
28	14094.0
29	15482.0
30	16870.0
31	19032.0
32	21194.0
33	21194.0
34	23363.0
35	25532.0
36	27707.0
37	29882.0
38	30538.5
39	31195.0
40	31195.0
41	32790.0
42	34385.0
43	34982.5
44	35580.0
45	36260.0
46	36940.0
47	36940.0
48	37258.5
49	37577.0
50	37737.5
51	37898.0
52	37420.5
53	36943.0
54	36943.0
55	35319.0
56	33695.0
57	32274.5
58	30854.0
59	30006.5
60	29159.0
61	29159.0
62	26957.5
63	24756.0
64	22668.0
65	20580.0
66	17772.5
67	14965.0
68	14965.0
69	13303.5
70	11642.0
71	9513.5
72	7385.0
73	5565.5
74	3746.0
75	3746.0
76	2849.5
77	1953.0
78	1558.0
79	1163.0
80	896.5
81	630.0
82	630.0
83	470.0
84	310.0
85	225.0
86	140.0
87	100.0
88	60.0
89	60.0
90	44.5
91	29.0
92	21.0
93	13.0
94	8.0
95	3.0
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	587970.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.33472519975304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82925862560317	63.36610753755194
2	7.06060445923408	10.073325576872278
3	1.6956087868052871	3.628673605591253
4	0.7045031089519197	2.010221427178274
5	0.38142159256045005	1.3604302245275934
6	0.2504570556360188	1.0719771142880794
7	0.18260037917007266	0.9118023508627489
8	0.12863669131022365	0.734101042017621
9	0.10488101851208703	0.6733492770806946
>10	0.5681752195387304	7.579089633312439
>50	0.06033413922690671	2.969874223574859
>100	0.03160355639669122	3.991367630640323
>500	9.576835271674411E-4	0.38820618846459254
>1k	9.576835271674411E-4	1.2414741680374497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.40529278704695815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2201	0.3743388268108917	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1451	0.24678129836556284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1234	0.20987465346871437	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	622	0.10578771025732604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.700767045937718E-4	0.0	0.0	0.0	0.0
6	1.700767045937718E-4	0.0	0.0	0.0	0.0
7	1.700767045937718E-4	0.0	0.0	0.0	0.0
8	1.700767045937718E-4	0.0	0.0	0.0	0.0
9	1.700767045937718E-4	0.0	0.0	0.0	0.0
10	3.401534091875436E-4	0.0	0.0	0.0	0.0
11	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
12	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
13	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
14	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
15	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
16	3.401534091875436E-4	1.700767045937718E-4	0.0	0.0	0.0
17	5.102301137813154E-4	1.700767045937718E-4	0.0	1.700767045937718E-4	0.0
18	5.102301137813154E-4	1.700767045937718E-4	0.0	1.700767045937718E-4	0.0
19	5.102301137813154E-4	1.700767045937718E-4	0.0	1.700767045937718E-4	0.0
20	5.102301137813154E-4	1.700767045937718E-4	0.0	3.401534091875436E-4	0.0
21	5.102301137813154E-4	1.700767045937718E-4	0.0	6.803068183750872E-4	0.0
22	5.102301137813154E-4	1.700767045937718E-4	0.0	0.001530690341343946	0.0
23	5.102301137813154E-4	1.700767045937718E-4	0.0	0.0028913039780941205	0.0
24	5.102301137813154E-4	1.700767045937718E-4	0.0	0.0066329914791571	0.0
25	5.102301137813154E-4	1.700767045937718E-4	0.0	0.011735292616970254	0.0
26	5.102301137813154E-4	1.700767045937718E-4	0.0	0.02262020171097165	0.0
27	5.102301137813154E-4	1.700767045937718E-4	0.0	0.0784053608177288	0.0
28	5.102301137813154E-4	1.700767045937718E-4	0.0	0.18436314777964863	0.0
29	5.102301137813154E-4	1.700767045937718E-4	0.0	0.30528768474582035	0.0
30	5.102301137813154E-4	1.700767045937718E-4	0.0	0.4299539092130551	0.0
31	5.102301137813154E-4	1.700767045937718E-4	0.0	0.6843886592853377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	435	0.0	31.045979	1
CGACCAA	55	1.9006098E-5	23.545454	12
GTTTAGG	40	0.0019300714	23.125	1
TAGAGCC	55	5.138988E-4	20.181818	4
GTATCAA	740	0.0	18.5	2
TATACTG	80	1.6144826E-5	18.5	5
TAGTCCG	60	9.2295E-4	18.5	5
TAAGCTA	105	4.7874164E-7	17.61905	33
GATATAC	65	0.0015786934	17.076923	3
CCACGAC	90	4.440015E-5	16.444445	9
GTATTAT	80	3.379051E-4	16.1875	1
CTTATAT	85	5.3586194E-4	15.235293	3
GGATAAG	110	1.4493917E-5	15.136364	1
ATACACT	160	1.092485E-8	15.03125	4
TATACTT	150	8.076677E-8	14.8	5
TTGTACT	140	5.9799095E-7	14.535714	4
TACACCA	115	2.206106E-5	14.478261	5
CACGACC	90	8.26554E-4	14.388889	10
ACGACCA	90	8.26554E-4	14.388889	11
TTTAGGG	130	4.434789E-6	14.230769	2
>>END_MODULE