FastQCFastQC Report
Fri 10 Feb 2017
ERR1630016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600949
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.8553138452680676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47120.7840931593196759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32750.5449713702826695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.3481160630935404No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.12064251708547646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG250.00549428329.59999825
GGTATCA13600.025.0294111
GTCCGTG952.8012437E-1023.3684227
TTAGTCG400.001930120423.1250025
CGTGCGA652.6793077E-622.7692310
CCGTGCG652.6793077E-622.769239
TCCGTGC806.9487396E-720.81258
CTAGTCC450.003823714620.5555573
TAGTCCG909.4594725E-820.5555575
AGTCCGT1103.8424332E-818.56
CGAGAAT852.7196147E-517.41176414
GTATCAA20850.016.4148672
GGACCTT803.3792056E-416.18756
CTAGAGG700.002590563815.8571423
GTGCGAG1059.328187E-615.85714211
CTTATAC5950.014.92437137
GGGTAAT750.004101375614.811
TGCGAGA1001.0919853E-414.79999912
GTGTAGT1152.2062655E-514.4782611
GTGTAAA1304.435178E-614.23076911
Produced by FastQC (version 0.11.5)