##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600949
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.134793468330926	33.0	31.0	34.0	31.0	34.0
2	32.38879339178533	33.0	31.0	34.0	31.0	34.0
3	32.54386478719492	34.0	31.0	34.0	31.0	34.0
4	36.1107980876913	37.0	35.0	37.0	35.0	37.0
5	35.987037169543505	37.0	35.0	37.0	35.0	37.0
6	36.08616870982396	37.0	35.0	37.0	35.0	37.0
7	36.01320910759482	37.0	35.0	37.0	35.0	37.0
8	36.02503706637335	37.0	35.0	37.0	35.0	37.0
9	37.6577147145598	39.0	37.0	39.0	35.0	39.0
10	37.54729103468015	39.0	37.0	39.0	35.0	39.0
11	37.6561155771954	39.0	37.0	39.0	35.0	39.0
12	37.52399454862226	39.0	37.0	39.0	35.0	39.0
13	37.594582901377656	39.0	37.0	39.0	35.0	39.0
14	38.79474963765644	40.0	38.0	41.0	35.0	41.0
15	38.77327194154579	40.0	38.0	41.0	35.0	41.0
16	38.66812824382768	40.0	38.0	41.0	35.0	41.0
17	38.630684134593785	40.0	38.0	41.0	35.0	41.0
18	38.517290152741744	40.0	38.0	41.0	34.0	41.0
19	38.566628782142914	40.0	38.0	41.0	34.0	41.0
20	38.75139154903328	40.0	38.0	41.0	35.0	41.0
21	38.72489845228131	40.0	38.0	41.0	35.0	41.0
22	38.71860673701096	40.0	38.0	41.0	35.0	41.0
23	38.64081644199425	40.0	38.0	41.0	35.0	41.0
24	38.56957911569867	40.0	38.0	41.0	34.0	41.0
25	38.54094773433353	40.0	38.0	41.0	34.0	41.0
26	38.337579395256505	40.0	38.0	41.0	34.0	41.0
27	38.17390494035268	40.0	38.0	41.0	34.0	41.0
28	38.08998600546802	40.0	38.0	41.0	34.0	41.0
29	37.91940580648274	40.0	37.0	41.0	33.0	41.0
30	37.78369878309141	40.0	37.0	41.0	33.0	41.0
31	37.58778531955291	40.0	37.0	41.0	33.0	41.0
32	37.46246353683923	39.0	36.0	41.0	33.0	41.0
33	37.31878745118138	39.0	36.0	40.0	33.0	41.0
34	37.210177569144804	39.0	36.0	40.0	33.0	41.0
35	37.01670191646878	39.0	36.0	40.0	32.0	41.0
36	36.939720342325224	39.0	36.0	40.0	31.0	41.0
37	36.8314948523086	39.0	36.0	40.0	31.0	41.0
38	36.68659902920214	39.0	35.0	40.0	31.0	41.0
39	36.56865224836051	39.0	35.0	40.0	30.0	41.0
40	36.41615178659087	39.0	35.0	40.0	30.0	41.0
41	36.217359542989506	39.0	35.0	40.0	30.0	41.0
42	36.038993325556746	39.0	35.0	40.0	29.0	41.0
43	35.67008847672598	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	6.0
17	19.0
18	26.0
19	62.0
20	126.0
21	233.0
22	381.0
23	656.0
24	1139.0
25	1728.0
26	2513.0
27	3627.0
28	4815.0
29	6490.0
30	8284.0
31	10324.0
32	13381.0
33	17371.0
34	23532.0
35	33628.0
36	53590.0
37	96723.0
38	175440.0
39	146848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10765139803877	20.318363122328183	13.394481062452886	23.179504417180162
2	18.957016319188483	23.000787088421813	34.79729561077563	23.24490098161408
3	19.786371222849194	24.10287728243162	31.713007260183478	24.397744234535708
4	13.917154367508724	16.96450114735194	36.00355437815855	33.11479010698079
5	13.54424418711072	37.70253382566574	34.77000544139353	13.983216545830013
6	32.519232081258146	36.75702929865929	15.272177838718425	15.451560781364144
7	27.175850196938512	31.807025221774225	22.29457075392421	18.722553827363054
8	26.247152420588105	34.545859964822306	20.314535842475816	18.892451772113773
9	25.10928548013226	14.787943735658102	21.152543726672313	38.950227057537326
10	16.61971315369524	28.088406836520235	34.10888444776512	21.182995562019403
11	33.52547387548694	22.94154745244605	22.552496135279366	20.980482536787648
12	19.986887406418845	27.444425400491557	30.948716114013003	21.619971079076596
13	29.903369503901327	21.307132552013567	25.6276322949202	23.161865649164902
14	20.711740929762758	23.105954082625978	28.085910784442607	28.096394203168657
15	23.346074292494038	29.55392221303305	24.739869772642937	22.36013372182997
16	21.926319870737785	28.282433284688054	26.718074245901068	23.073172598673096
17	21.43892410171246	28.21087979179598	27.947463095870035	22.402733010621535
18	21.504653473090062	26.66049864464372	29.116946695975866	22.71790118629035
19	22.758337229947966	26.766830463150782	29.90253748654212	20.572294820359133
20	22.824565811741095	26.73221854100764	29.27436438033843	21.16885126691283
21	22.013016079567485	27.139740643548787	29.18134483957873	21.665898437304996
22	22.277764003268164	27.034407245872778	29.445926359807572	21.241902391051486
23	21.816992789737565	27.013939618836208	29.252731928998966	21.91633566242726
24	21.799686828665994	27.2533942148169	29.06070232249326	21.886216634023853
25	21.989553190037757	27.078004955495395	29.163539668091637	21.768902186375215
26	21.718315530935236	27.475210042782333	29.068190478726148	21.73828394755628
27	21.615810992280544	27.602675102213333	29.096479068939296	21.685034836566828
28	21.38267972822985	27.747279719244062	29.443929518145467	21.42611103438062
29	21.550747234790308	27.761257610878793	29.713170335585882	20.97482481874502
30	21.552078462565046	27.704514026980657	29.291171130994474	21.452236379459823
31	21.32893140682487	27.53028959196205	29.42878680220784	21.71199219900524
32	21.138565835037582	27.63445816533516	29.6020128163954	21.624963183231856
33	21.14988127112284	27.451081539365234	29.528795288784902	21.870241900727017
34	21.649424493592633	26.943717353718867	29.520475115192802	21.886383037495694
35	21.326601758219084	27.018765319519627	29.616157111501973	22.038475810759316
36	21.19580862935124	26.764833621488677	29.516647835340436	22.522709913819643
37	20.88097326062611	26.11802332643868	30.64486337442944	22.356140038505764
38	20.53385561836362	26.073427195985015	30.894634985664343	22.49808219998702
39	20.291405759889773	26.32835731484702	31.338932255482575	22.041304669780633
40	20.265613221754258	25.95910801082954	31.443600039271217	22.331678728144986
41	19.899359180229936	26.00387054475505	31.48936099402778	22.60740928098724
42	19.360711141877264	26.476290001314588	31.252735257068405	22.910263599739743
43	19.503651724189574	25.88239601031036	31.34542199088442	23.26853027461565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	958.0
1	744.0
2	530.0
3	878.0
4	1226.0
5	1226.0
6	1534.5
7	1843.0
8	1834.5
9	1826.0
10	2345.5
11	2865.0
12	2865.0
13	4758.0
14	6651.0
15	9257.5
16	11864.0
17	11225.0
18	10586.0
19	10586.0
20	12157.5
21	13729.0
22	12084.5
23	10440.0
24	11886.0
25	13332.0
26	13332.0
27	14414.5
28	15497.0
29	17032.5
30	18568.0
31	20590.5
32	22613.0
33	22613.0
34	25012.0
35	27411.0
36	29565.0
37	31719.0
38	32045.5
39	32372.0
40	32372.0
41	33162.0
42	33952.0
43	34990.0
44	36028.0
45	35557.0
46	35086.0
47	35086.0
48	35325.5
49	35565.0
50	36041.0
51	36517.0
52	36482.0
53	36447.0
54	36447.0
55	35738.0
56	35029.0
57	32923.0
58	30817.0
59	28695.0
60	26573.0
61	26573.0
62	24100.0
63	21627.0
64	18524.5
65	15422.0
66	13482.5
67	11543.0
68	11543.0
69	9986.0
70	8429.0
71	7402.0
72	6375.0
73	5247.5
74	4120.0
75	4120.0
76	2860.5
77	1601.0
78	1242.0
79	883.0
80	684.0
81	485.0
82	485.0
83	351.0
84	217.0
85	172.0
86	127.0
87	86.0
88	45.0
89	45.0
90	32.5
91	20.0
92	12.5
93	5.0
94	4.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	600949.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.07407671508304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.05667136292337	64.34659958449119
2	8.164725890314603	11.93259612132948
3	1.7693143489327088	3.878730374010178
4	0.6404564207432054	1.8720304648822597
5	0.3548794637713877	1.2966244580118957
6	0.20011853122935763	0.8774086141898292
7	0.13844035250402364	0.7081480657539514
8	0.09679361651948427	0.5658483327260098
9	0.07584996990134471	0.4988399867466842
>10	0.4316561397847156	5.860160198230468
>50	0.04480520301789895	2.1909724399409134
>100	0.024002726413782784	2.8369326287921015
>500	0.0013715843664869412	0.5928442501037324
>1k	6.857921832434706E-4	1.6836509430249418
>5k	2.285973944144902E-4	0.8586135377664651
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5140	0.8553138452680676	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4712	0.7840931593196759	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.5449713702826695	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.3481160630935404	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.12064251708547646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6640347184203652E-4
2	0.0	0.0	0.0	0.0	1.6640347184203652E-4
3	0.0	0.0	0.0	0.0	1.6640347184203652E-4
4	0.0	0.0	0.0	0.0	3.3280694368407305E-4
5	0.0	0.0	0.0	0.0	3.3280694368407305E-4
6	0.0	0.0	0.0	0.0	3.3280694368407305E-4
7	0.0	0.0	0.0	0.0	3.3280694368407305E-4
8	0.0	0.0	0.0	0.0	3.3280694368407305E-4
9	0.0	0.0	0.0	1.6640347184203652E-4	3.3280694368407305E-4
10	0.0	0.0	0.0	8.320173592101825E-4	3.3280694368407305E-4
11	1.6640347184203652E-4	0.0	0.0	8.320173592101825E-4	3.3280694368407305E-4
12	1.6640347184203652E-4	0.0	0.0	0.0011648243028942555	3.3280694368407305E-4
13	1.6640347184203652E-4	0.0	0.0	0.0011648243028942555	3.3280694368407305E-4
14	1.6640347184203652E-4	0.0	0.0	0.0011648243028942555	3.3280694368407305E-4
15	1.6640347184203652E-4	0.0	0.0	0.0013312277747362922	3.3280694368407305E-4
16	1.6640347184203652E-4	0.0	0.0	0.0014976312465783286	3.3280694368407305E-4
17	1.6640347184203652E-4	0.0	0.0	0.0014976312465783286	3.3280694368407305E-4
18	1.6640347184203652E-4	0.0	0.0	0.001664034718420365	3.3280694368407305E-4
19	1.6640347184203652E-4	0.0	0.0	0.0018304381902624017	3.3280694368407305E-4
20	1.6640347184203652E-4	0.0	0.0	0.002329648605788511	3.3280694368407305E-4
21	1.6640347184203652E-4	0.0	0.0	0.00332806943684073	4.992104155261095E-4
22	1.6640347184203652E-4	0.0	0.0	0.005824121514471278	4.992104155261095E-4
23	1.6640347184203652E-4	0.0	0.0	0.007321752761049607	4.992104155261095E-4
24	1.6640347184203652E-4	0.0	0.0	0.013478681219204957	4.992104155261095E-4
25	1.6640347184203652E-4	0.0	0.0	0.02196525828314882	4.992104155261095E-4
26	1.6640347184203652E-4	0.0	0.0	0.03860560546735247	4.992104155261095E-4
27	1.6640347184203652E-4	0.0	0.0	0.11781365806416184	4.992104155261095E-4
28	1.6640347184203652E-4	0.0	0.0	0.2484403834601605	4.992104155261095E-4
29	1.6640347184203652E-4	0.0	0.0	0.41168218933719836	4.992104155261095E-4
30	1.6640347184203652E-4	0.0	0.0	0.6075390756952753	4.992104155261095E-4
31	1.6640347184203652E-4	0.0	0.0	0.9809484665088053	4.992104155261095E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTACG	25	0.005494283	29.599998	25
GGTATCA	1360	0.0	25.029411	1
GTCCGTG	95	2.8012437E-10	23.368422	7
TTAGTCG	40	0.0019301204	23.125002	5
CGTGCGA	65	2.6793077E-6	22.76923	10
CCGTGCG	65	2.6793077E-6	22.76923	9
TCCGTGC	80	6.9487396E-7	20.8125	8
CTAGTCC	45	0.0038237146	20.555557	3
TAGTCCG	90	9.4594725E-8	20.555557	5
AGTCCGT	110	3.8424332E-8	18.5	6
CGAGAAT	85	2.7196147E-5	17.411764	14
GTATCAA	2085	0.0	16.414867	2
GGACCTT	80	3.3792056E-4	16.1875	6
CTAGAGG	70	0.0025905638	15.857142	3
GTGCGAG	105	9.328187E-6	15.857142	11
CTTATAC	595	0.0	14.924371	37
GGGTAAT	75	0.0041013756	14.8	11
TGCGAGA	100	1.0919853E-4	14.799999	12
GTGTAGT	115	2.2062655E-5	14.478261	1
GTGTAAA	130	4.435178E-6	14.230769	11
>>END_MODULE