FastQCFastQC Report
Fri 10 Feb 2017
ERR1630015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105884
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44290.4004940843705126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.289451696561303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26610.24062198205236715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.16728698489172464No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT12520.11321259734294012No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG12010.10860090208376284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.028.6082481
CTGTCGC609.236513E-418.59
CGAATTA1950.018.02564215
TAGACTC1351.1514203E-917.8148165
GTATCAA15800.017.680382
GTCTAGA752.0671774E-417.2666661
TTAGACT1452.9776857E-916.5862084
TACACTG2050.016.2439025
CCCGAAT2300.016.08695613
ACTGCGT700.002592410415.8571428
CCGAATT2350.015.74467914
CCTACAC1455.344009E-815.3103463
GTAATAG1101.45169E-515.1363641
ATTAGAC750.004104275714.83
TGCGTTA1152.2095835E-514.47826137
AATCTCG3750.014.30666610
GGACTAC3150.014.0952391
GCGTTTA1458.9083005E-714.03448425
ATCTCGC4100.013.98780411
CTGAGTA5300.013.9622639
Produced by FastQC (version 0.11.5)