##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630015.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105884
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66658618806312	34.0	31.0	34.0	31.0	34.0
2	32.91059912251195	34.0	31.0	34.0	31.0	34.0
3	33.05026566981709	34.0	33.0	34.0	31.0	34.0
4	36.411619120992796	37.0	37.0	37.0	35.0	37.0
5	36.336071414361726	37.0	37.0	37.0	35.0	37.0
6	36.394158881039964	37.0	37.0	37.0	35.0	37.0
7	36.33051929497126	37.0	37.0	37.0	35.0	37.0
8	36.33598912725024	37.0	37.0	37.0	35.0	37.0
9	38.0669536768775	39.0	38.0	39.0	35.0	39.0
10	38.03154489982675	39.0	38.0	39.0	35.0	39.0
11	38.08548455353365	39.0	38.0	39.0	37.0	39.0
12	38.01211429046808	39.0	38.0	39.0	35.0	39.0
13	38.037002976804075	39.0	38.0	39.0	35.0	39.0
14	39.441243385382194	41.0	39.0	41.0	37.0	41.0
15	39.44230317103783	41.0	39.0	41.0	37.0	41.0
16	39.3557244701976	41.0	39.0	41.0	36.0	41.0
17	39.28276564268947	40.0	39.0	41.0	36.0	41.0
18	39.173133891077185	40.0	39.0	41.0	36.0	41.0
19	39.21242824744729	40.0	39.0	41.0	36.0	41.0
20	39.41938394985369	41.0	39.0	41.0	37.0	41.0
21	39.41565118945567	41.0	39.0	41.0	37.0	41.0
22	39.437509720730205	41.0	39.0	41.0	37.0	41.0
23	39.37527805809651	41.0	39.0	41.0	36.0	41.0
24	39.33000296595303	41.0	39.0	41.0	36.0	41.0
25	39.306400128765766	41.0	39.0	41.0	36.0	41.0
26	39.167270708320224	40.0	39.0	41.0	36.0	41.0
27	39.06728734659331	40.0	39.0	41.0	36.0	41.0
28	39.03338324815261	40.0	39.0	41.0	35.0	41.0
29	38.922455700597894	40.0	39.0	41.0	35.0	41.0
30	38.84044619508013	40.0	39.0	41.0	35.0	41.0
31	38.712168726557216	40.0	38.0	41.0	35.0	41.0
32	38.623956038788876	40.0	38.0	41.0	35.0	41.0
33	38.552413273001505	40.0	38.0	41.0	35.0	41.0
34	38.48510512856683	40.0	38.0	41.0	35.0	41.0
35	38.35087043487382	40.0	38.0	41.0	35.0	41.0
36	38.33796582643387	40.0	38.0	41.0	35.0	41.0
37	38.29522897519089	40.0	38.0	41.0	34.0	41.0
38	38.184551905986524	40.0	38.0	41.0	34.0	41.0
39	38.138617612697175	40.0	38.0	41.0	34.0	41.0
40	38.07449153799133	40.0	38.0	41.0	34.0	41.0
41	37.96593946562207	40.0	38.0	41.0	34.0	41.0
42	37.87437199561618	40.0	38.0	41.0	33.0	41.0
43	37.57182760578867	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	2.0
15	2.0
16	11.0
17	22.0
18	45.0
19	77.0
20	126.0
21	228.0
22	409.0
23	762.0
24	1167.0
25	1775.0
26	2670.0
27	3714.0
28	5168.0
29	6784.0
30	9192.0
31	11867.0
32	15127.0
33	19934.0
34	27097.0
35	37934.0
36	58365.0
37	105350.0
38	248217.0
39	549835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51693667690282	20.175714631914378	12.651236476881845	24.656112214300958
2	18.329318445695932	22.416184699299386	35.77509033497184	23.479406520032843
3	19.16222677966224	24.13408639604154	32.072622445030404	24.63106437926582
4	14.41597852939368	16.484007364244352	36.51766369709662	32.582350409265345
5	13.480075667972411	37.3887315486977	35.69696279175754	13.434229991572352
6	32.449605926118835	36.77664203478846	15.827880681879837	14.945871357212873
7	26.52095518155611	31.75595270390023	22.960003038293348	18.76308907625031
8	24.973595784006278	35.173942294128494	21.12762278864691	18.724839133218314
9	24.86996827877065	15.146525313685702	21.487063742671022	38.496442664872625
10	15.765125456196131	28.1013198490981	35.55164917839484	20.581905516310933
11	32.956801979231095	23.055401832380248	23.400374722846156	20.587421465542498
12	19.378072202871188	26.84974192591628	31.23492156501044	22.537264306202097
13	28.40542046001208	21.747850588307635	26.297333174184633	23.54939577749565
14	20.908250774945657	23.202523953687727	26.90725247856014	28.981972792806477
15	23.387986443424445	29.872301254019405	24.303272314275276	22.436439988280867
16	22.705545970463447	28.715669997938303	25.87287635954585	22.7059076720524
17	21.77597288684889	28.358760955036878	27.477294182753344	22.387971975360887
18	21.286138510006474	27.313895489942887	28.74053698217896	22.659429017871677
19	22.590163163586777	27.158725508281158	29.13849915542679	21.112612172705276
20	22.744790592865076	26.93356626915662	29.001956805596247	21.319686332382055
21	21.79677072821381	27.755261853865328	28.062346502888186	22.385620915032682
22	22.007281052985668	27.35323053774175	28.320872713593833	22.31861569567875
23	22.03124378325394	27.453150601690595	28.207569690853653	22.308035924201818
24	22.85483830130466	27.302773166082517	28.112713449150185	21.72967508346264
25	22.624796090729227	26.8661089228165	28.386883253578134	22.122211732876142
26	22.325759302060614	27.344459274209594	28.10954856024683	22.22023286348297
27	22.04064802456677	27.445374017528056	27.901660571994892	22.612317385910277
28	21.838366410943642	27.42068788408187	28.44439380622199	22.296551898752494
29	22.039472494402666	27.70570873617848	28.500367127112792	21.754451642306062
30	21.7783239471771	28.246271760871842	28.125644280955324	21.849760010995727
31	22.298360406697267	27.361730525082194	28.613941426044686	21.725967642175853
32	21.60678696861515	27.62938970090896	28.52315432721696	22.24066900325893
33	21.90464822711966	27.296262537481326	28.530840485982257	22.268248749416756
34	21.912424811282197	27.080326688875147	28.519808587519123	22.487439912323534
35	22.085951148583398	27.12861385100065	28.609239305388268	22.17619569502769
36	21.905823757283766	27.14262978757266	28.60905845459379	22.342488000549785
37	22.283711492344587	26.053003750845477	28.800127318959312	22.863157437850624
38	21.788903718654037	26.593476350141604	29.521089011144024	22.09653092006033
39	21.444292529777083	26.5290934673076	29.810811983897047	22.21580201901827
40	21.291835310032518	25.698264917477783	30.335912265662586	22.673987506827117
41	20.81791580310412	26.10499835425777	30.29856657660297	22.778519266035136
42	20.933117759186317	25.60241399640469	30.086790296269772	23.377677948139226
43	20.54094281136177	25.8394189625675	30.25896025261239	23.360677973458337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1211.0
1	941.0
2	671.0
3	1127.0
4	1583.0
5	1583.0
6	1850.5
7	2118.0
8	1901.5
9	1685.0
10	2348.5
11	3012.0
12	3012.0
13	4502.5
14	5993.0
15	8087.5
16	10182.0
17	10685.0
18	11188.0
19	11188.0
20	14399.0
21	17610.0
22	18607.5
23	19605.0
24	23090.0
25	26575.0
26	26575.0
27	28168.0
28	29761.0
29	35756.0
30	41751.0
31	44008.5
32	46266.0
33	46266.0
34	49107.0
35	51948.0
36	55966.5
37	59985.0
38	62704.0
39	65423.0
40	65423.0
41	69853.0
42	74283.0
43	71083.5
44	67884.0
45	69123.0
46	70362.0
47	70362.0
48	70741.5
49	71121.0
50	73283.5
51	75446.0
52	77021.0
53	78596.0
54	78596.0
55	70116.0
56	61636.0
57	57101.0
58	52566.0
59	47207.5
60	41849.0
61	41849.0
62	38063.0
63	34277.0
64	30247.5
65	26218.0
66	22723.5
67	19229.0
68	19229.0
69	16359.0
70	13489.0
71	11435.5
72	9382.0
73	7714.5
74	6047.0
75	6047.0
76	4597.5
77	3148.0
78	2530.0
79	1912.0
80	1427.0
81	942.0
82	942.0
83	722.0
84	502.0
85	366.5
86	231.0
87	166.0
88	101.0
89	101.0
90	74.0
91	47.0
92	36.5
93	26.0
94	20.5
95	15.0
96	15.0
97	10.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1105884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.11218395263406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6053854716687	53.792496337050665
2	8.520069942108389	10.584003030670889
3	2.0319176364357867	3.78620526032703
4	0.8433911025505493	2.0953945322253826
5	0.4505385275497786	1.3991965950460368
6	0.273875579391011	1.0206606220361225
7	0.1802645166084365	0.7837635971001107
8	0.13481077191715587	0.6698713171291983
9	0.10459935183744022	0.5847204764388041
>10	0.6834652751307706	8.281220406984671
>50	0.08512269756299728	3.7048676845416804
>100	0.08010387140056273	9.533772782750354
>500	0.005281572958805099	2.247598338602737
>1k	0.0011736828797344664	1.5162290190963283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4429	0.4004940843705126	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3201	0.289451696561303	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2661	0.24062198205236715	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	0.16728698489172464	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1252	0.11321259734294012	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1201	0.10860090208376284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8085079447754013E-4	0.0
2	0.0	0.0	0.0	1.8085079447754013E-4	0.0
3	0.0	0.0	0.0	1.8085079447754013E-4	0.0
4	0.0	0.0	0.0	1.8085079447754013E-4	0.0
5	0.0	0.0	0.0	1.8085079447754013E-4	0.0
6	0.0	0.0	0.0	1.8085079447754013E-4	0.0
7	0.0	0.0	0.0	1.8085079447754013E-4	0.0
8	0.0	0.0	0.0	1.8085079447754013E-4	0.0
9	0.0	0.0	0.0	2.7127619171631024E-4	0.0
10	0.0	0.0	0.0	2.7127619171631024E-4	0.0
11	0.0	0.0	0.0	3.6170158895508026E-4	0.0
12	0.0	0.0	0.0	4.5212698619385034E-4	0.0
13	0.0	0.0	0.0	4.5212698619385034E-4	0.0
14	0.0	0.0	0.0	4.5212698619385034E-4	0.0
15	0.0	0.0	0.0	4.5212698619385034E-4	0.0
16	0.0	0.0	0.0	4.5212698619385034E-4	0.0
17	0.0	0.0	0.0	5.425523834326205E-4	0.0
18	0.0	0.0	0.0	5.425523834326205E-4	0.0
19	0.0	0.0	0.0	7.234031779101605E-4	0.0
20	0.0	0.0	0.0	9.946793696264708E-4	0.0
21	0.0	0.0	0.0	0.001085104766865241	0.0
22	0.0	0.0	0.0	0.0017180825475366314	0.0
23	0.0	0.0	0.0	0.0032553143005957224	0.0
24	0.0	9.042539723877007E-5	0.0	0.006329777806713905	0.0
25	0.0	9.042539723877007E-5	0.0	0.01193615243551765	0.0
26	0.0	9.042539723877007E-5	0.0	0.023420177884841447	0.0
27	0.0	9.042539723877007E-5	0.0	0.07423925113303023	0.0
28	0.0	9.042539723877007E-5	0.0	0.15218594355285003	0.0
29	0.0	9.042539723877007E-5	0.0	0.23528688361527972	0.0
30	0.0	9.042539723877007E-5	0.0	0.32426547449822946	0.0
31	0.0	9.042539723877007E-5	0.0	0.48052056092682416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	970	0.0	28.608248	1
CTGTCGC	60	9.236513E-4	18.5	9
CGAATTA	195	0.0	18.025642	15
TAGACTC	135	1.1514203E-9	17.814816	5
GTATCAA	1580	0.0	17.68038	2
GTCTAGA	75	2.0671774E-4	17.266666	1
TTAGACT	145	2.9776857E-9	16.586208	4
TACACTG	205	0.0	16.243902	5
CCCGAAT	230	0.0	16.086956	13
ACTGCGT	70	0.0025924104	15.857142	8
CCGAATT	235	0.0	15.744679	14
CCTACAC	145	5.344009E-8	15.310346	3
GTAATAG	110	1.45169E-5	15.136364	1
ATTAGAC	75	0.0041042757	14.8	3
TGCGTTA	115	2.2095835E-5	14.478261	37
AATCTCG	375	0.0	14.306666	10
GGACTAC	315	0.0	14.095239	1
GCGTTTA	145	8.9083005E-7	14.034484	25
ATCTCGC	410	0.0	13.987804	11
CTGAGTA	530	0.0	13.962263	9
>>END_MODULE