FastQCFastQC Report
Fri 10 Feb 2017
ERR1630014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430710
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57280.4003606600918425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50220.35101453124672366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40020.2797212572778551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.20472352887727072No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG14900.10414409628785708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.027.785441
TTAACGG759.2683695E-619.73333435
ATGCGTT1258.583811E-917.76000236
GTATCAA20850.017.4796162
TGCGTTA1401.869921E-917.17857237
AAATGCG1452.9813236E-916.58620634
TAACGGC904.4471788E-516.44444536
TAAACCG700.002592909415.8571435
AATGCGT1455.347829E-815.31034535
GTACATA1853.0559022E-1015.01
TACGGTC750.004105058514.810
ATCCGCG4000.014.7999997
TGCGTTT1904.5474735E-1014.60526424
GTACTAA1152.2104805E-514.4782611
GTATAGG1803.3305696E-914.3888891
GTGTAGA2450.014.3469381
GCGCCTA4400.014.29545411
CGTGCGA1051.6560778E-414.09523810
CGCCCGA1051.6560778E-414.09523810
TAATACG800.00629961413.8750014
Produced by FastQC (version 0.11.5)