FastQCFastQC Report
Fri 10 Feb 2017
ERR1630013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17947
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5573.103582771493843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4722.6299660110324847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3231.799743689753162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2131.1868278820972864No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.445756951022455No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.37332144648130605No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.3231737894912799No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3008859419401571No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.24516632306235026No Hit
AGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAA430.23959436117456956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG370.20616258984788544No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.19501866607232407No Hit
GAGTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAA300.16715885663342062No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA300.16715885663342062No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1392990471945172No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1392990471945172No Hit
GTACGGGGGAGGCAGTACAGGCACTAAAAAAAAAAAAAAAAAA250.1392990471945172No Hit
GGTATCAACGCAGAGTACGGGGGAGGCAGTACAGGCACTGTCT240.1337270853067365No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT220.12258316153117513RNA PCR Primer, Index 46 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT210.11701119964339445No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11143923775561375No Hit
TGCCTGTACTGCCTCCCCCGTACTCTGCGTTGATACCCTGTCT200.11143923775561375No Hit
GAGTACGGGGGAGGCAGTACAGGCAAAAAAAAAAAAAAAAAAA190.10586727586783308No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT190.10586727586783308No Hit
GTACAGGCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC180.10029531398005238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT400.001858316223.1254
CTCTTAT700.00246134115.85714337
GGTATCA2700.015.0740731
TCTCTTA850.00894037513.05882436
AACGCAG3350.011.5970157
CGCAGAG3550.011.4647889
GTATCAA3401.8189894E-1211.4264712
AGTACGG2351.0765689E-711.0212772
GAGTACG2351.0765689E-711.0212771
GCAGAGT3700.011.010
CAGAGTA3750.010.853332511
AGAGTAC3800.010.71052612
GTACGGG2452.2638942E-69.8163263
ACGCAGA4053.274181E-119.5925928
CAACGCA4351.3460522E-108.9310346
GAGTACT2503.162281E-58.886
TATCAAC4452.1100277E-108.7303373
AGTACTT2553.9933104E-58.7058827
ATCAACG4604.0745363E-108.4456524
GTACTTT2901.93018E-58.2931048
Produced by FastQC (version 0.11.5)