FastQCFastQC Report
Thu 5 Jan 2017
ERR1629988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28878254
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC1202360.416354811478561No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG932680.3229696643017268No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC629230.21789059684841058No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG520930.18038832957144846No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC463820.16061220321699504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG180000.036.9061437
GAACGCT194000.034.033259
AACGCTG195250.033.8879710
CGTGACG30700.031.7760770
GTCGAAC213550.030.6645339
GGCCTAA216850.030.62417223
TCGAACG213850.030.54452940
ACGGTAA215600.030.40247544
CGGTAAC221400.029.56211345
ATTGAAC236650.029.4316396
GACAGGC317150.029.429422
GCCTAAC227700.029.07561524
TGAACGC232000.029.025718
AGTCGAA230100.028.6376138
GAACGGT231250.028.32590542
CTCAGAT281650.027.727151
CAAGTCG239150.027.5909636
ACGCTGG243500.027.41576411
AACGGTA239150.027.39228843
GGACAGG342950.027.3718431
Produced by FastQC (version 0.11.5)