FastQCFastQC Report
Thu 5 Jan 2017
ERR1629988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28878254
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA373590.12936723944598588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC92200.035.84401370
AACGCTC73300.029.83031563
AGAACGC74950.029.10774861
CCCCTAC90600.027.0828769
CGCCTTA105500.027.01731164
TAGGGGT124600.025.91469869
AGGGGTC174350.025.17377970
GAACGCT88000.025.11552862
ACGCTCC97150.022.91133564
CAGAACG98250.022.62263160
ACCTTCG70350.022.57736645
ACGGGGC97600.021.79057170
GTGTACG64450.021.63679766
TTCGCAC171300.021.1271618
GCCTTAG135850.021.0939865
TCGCACT106000.020.970539
CGGTTCG90200.020.8339633
TGCGCTA20150.020.74104759
CGTATCG31700.020.2718623
CCCGTAT37500.020.198681
Produced by FastQC (version 0.11.5)