FastQCFastQC Report
Thu 5 Jan 2017
ERR1629987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602176
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC197260.25947833883351296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141150.18567052380792026No Hit
AGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGAC136050.17896191827182112No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC97800.1286473767510776No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG87570.11519070329337285No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC81960.1078112372036638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC32950.036.576739
TTGAACG35050.035.418467
TCGAACG34350.035.39184640
GAACGCT34750.034.8332529
ACGGTAA35400.034.1559944
GGCCTAA37550.033.28299723
AACGCTG36750.033.12893710
GAACGGT37300.032.6919242
AGTCGAA37100.032.6703138
AACGGTA37650.032.113143
CGGTAAC37750.031.93957945
GCCTAAC38800.031.67451724
CGAACGG38800.031.15779741
CAAGTCG39000.030.98574636
AAGTCGA41650.029.5996437
ATTGAAC46400.028.2445166
TGAACGC44700.027.6988148
TAACACA45450.026.96546427
CTCAGAT63700.025.681471
GTTTCGC15400.025.3390767
Produced by FastQC (version 0.11.5)