FastQCFastQC Report
Thu 5 Jan 2017
ERR1629987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7602176
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAATTCATCGTGACGTTTGGCAATGTTATTCCTGTTCCTCTTGGTAT99060.13030479694234914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC17550.026.72546670
AGAACGC13050.025.09967461
AACGCTC13350.024.03469863
CCCGTAT8250.021.9008451
TGCGACG12100.021.24337610
CGGTTCG14450.020.4357763
GTATCGA7450.020.0514834
ACGTTAT13850.019.31159414
TTCGCAC26300.019.150668
CGTATCG8550.019.0972923
CCCCTAC19050.018.91001169
CGCCTTA20300.018.90543264
GACGTTA14450.018.75011413
TTGCGAC15950.018.7288919
TCGCACT17500.018.459479
TCTATCG3600.018.39789462
CTATCGC3450.018.19640263
GGTTCGG12450.018.1375394
CAGAACG18500.017.70042460
GAACGCT18450.017.5712962
Produced by FastQC (version 0.11.5)