FastQCFastQC Report
Thu 5 Jan 2017
ERR1629986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38556004
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG741480.19231246059627966No Hit
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC716840.18592175682936438No Hit
AGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGAC396410.10281407793193506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC75250.031.97956739
TCGAACG80000.030.1286540
ACGGTAA81000.029.7245644
AACGGTA82200.029.3314843
CGAACGG87500.027.51022541
CGGTAAC88150.027.11845645
AGTCGAA95700.025.57962838
TTGAACG105150.024.4671737
GAACGGT109000.022.62749142
GAACGCT108550.022.5857819
AACGCTG115050.021.55182610
GCCTAAC114000.021.27656624
GGCCTAA117750.021.15764623
AAGTCGA118000.020.83219337
CAAGTCG117950.020.60282536
GACAGGC286250.019.2489932
TGAACGC142550.017.7332978
TAGGTCG16000.017.545873
GGACAGG317850.017.5447921
GTTTCGC47050.017.27927267
Produced by FastQC (version 0.11.5)